Tags

Type your tag names separated by a space and hit enter

Knockdown of RNA interference pathway genes in western corn rootworm, Diabrotica virgifera virgifera, identifies no fitness costs associated with Argonaute 2 or Dicer-2.
Pestic Biochem Physiol. 2018 Jun; 148:103-110.PB

Abstract

The use of transgenic crops that induce silencing of essential genes using double-stranded RNA (dsRNA) through RNA interference (RNAi) in western corn rootworm, Diabrotica virgifera virgifera, is likely to be an important component of new technologies for the control of this important corn pest. Previous studies have demonstrated that the dsRNA response in D. v. virgifera depends on the presence of RNAi pathway genes including Dicer-2 and Argonaute 2, and that downregulation of these genes limits the lethality of environmental dsRNA. A potential resistance mechanism to lethal dsRNA may involve loss of function of RNAi pathway genes. Howver, the potential for resistance to evolve may depend on whether these pathway genes have essential functions such that the loss of function of core proteins in the RNAi pathway will have fitness costs in D. v. virgifera. Fitness costs associated with potential resistance mechanisms have a central role in determining how resistance can evolve to RNAi technologies in western corn rootworm. We evaluated the effect of dsRNA and microRNA pathway gene knockdown on the development of D. v. virgifera larvae through short-term and long-term exposures to dsRNA for Dicer and Argonaute genes. Downregulation of Argonaute 2, Dicer-2, Dicer-1 did not significantly affect larval survivorship or development through short and long-term exposure to dsRNA. However, downregulation of Argonaute 1 reduced larval survivorship and delayed development. The implications of these results as they relate to D. v. virgifera resistance to lethal dsRNA are discussed.

Authors+Show Affiliations

Department of Entomology and Nematology, University of Florida, 1881 Natural Area Drive, Steinmetz Hall, Gainesville, FL 32611, United States.Department of Entomology and Nematology, University of Florida, 1881 Natural Area Drive, Steinmetz Hall, Gainesville, FL 32611, United States.Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN 46268, United States.Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN 46268, United States.Department of Entomology and Nematology, University of Florida, 1881 Natural Area Drive, Steinmetz Hall, Gainesville, FL 32611, United States. Electronic address: bsiegfried1@ufl.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29891360

Citation

Camargo, Carolina, et al. "Knockdown of RNA Interference Pathway Genes in Western Corn Rootworm, Diabrotica Virgifera Virgifera, Identifies No Fitness Costs Associated With Argonaute 2 or Dicer-2." Pesticide Biochemistry and Physiology, vol. 148, 2018, pp. 103-110.
Camargo C, Wu K, Fishilevich E, et al. Knockdown of RNA interference pathway genes in western corn rootworm, Diabrotica virgifera virgifera, identifies no fitness costs associated with Argonaute 2 or Dicer-2. Pestic Biochem Physiol. 2018;148:103-110.
Camargo, C., Wu, K., Fishilevich, E., Narva, K. E., & Siegfried, B. D. (2018). Knockdown of RNA interference pathway genes in western corn rootworm, Diabrotica virgifera virgifera, identifies no fitness costs associated with Argonaute 2 or Dicer-2. Pesticide Biochemistry and Physiology, 148, 103-110. https://doi.org/10.1016/j.pestbp.2018.04.004
Camargo C, et al. Knockdown of RNA Interference Pathway Genes in Western Corn Rootworm, Diabrotica Virgifera Virgifera, Identifies No Fitness Costs Associated With Argonaute 2 or Dicer-2. Pestic Biochem Physiol. 2018;148:103-110. PubMed PMID: 29891360.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Knockdown of RNA interference pathway genes in western corn rootworm, Diabrotica virgifera virgifera, identifies no fitness costs associated with Argonaute 2 or Dicer-2. AU - Camargo,Carolina, AU - Wu,Ke, AU - Fishilevich,Elane, AU - Narva,Kenneth E, AU - Siegfried,Blair D, Y1 - 2018/04/09/ PY - 2017/12/05/received PY - 2018/04/07/revised PY - 2018/04/07/accepted PY - 2018/6/13/entrez PY - 2018/6/13/pubmed PY - 2018/9/6/medline KW - Argonaute KW - Diabrotica KW - Dicer KW - Drosha KW - Fitness KW - RNAi KW - Rootworm SP - 103 EP - 110 JF - Pesticide biochemistry and physiology JO - Pestic Biochem Physiol VL - 148 N2 - The use of transgenic crops that induce silencing of essential genes using double-stranded RNA (dsRNA) through RNA interference (RNAi) in western corn rootworm, Diabrotica virgifera virgifera, is likely to be an important component of new technologies for the control of this important corn pest. Previous studies have demonstrated that the dsRNA response in D. v. virgifera depends on the presence of RNAi pathway genes including Dicer-2 and Argonaute 2, and that downregulation of these genes limits the lethality of environmental dsRNA. A potential resistance mechanism to lethal dsRNA may involve loss of function of RNAi pathway genes. Howver, the potential for resistance to evolve may depend on whether these pathway genes have essential functions such that the loss of function of core proteins in the RNAi pathway will have fitness costs in D. v. virgifera. Fitness costs associated with potential resistance mechanisms have a central role in determining how resistance can evolve to RNAi technologies in western corn rootworm. We evaluated the effect of dsRNA and microRNA pathway gene knockdown on the development of D. v. virgifera larvae through short-term and long-term exposures to dsRNA for Dicer and Argonaute genes. Downregulation of Argonaute 2, Dicer-2, Dicer-1 did not significantly affect larval survivorship or development through short and long-term exposure to dsRNA. However, downregulation of Argonaute 1 reduced larval survivorship and delayed development. The implications of these results as they relate to D. v. virgifera resistance to lethal dsRNA are discussed. SN - 1095-9939 UR - https://www.unboundmedicine.com/medline/citation/29891360/Knockdown_of_RNA_interference_pathway_genes_in_western_corn_rootworm_Diabrotica_virgifera_virgifera_identifies_no_fitness_costs_associated_with_Argonaute_2_or_Dicer_2_ DB - PRIME DP - Unbound Medicine ER -