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Live-attenuated bivalent measles virus-derived vaccines targeting Middle East respiratory syndrome coronavirus induce robust and multifunctional T cell responses against both viruses in an appropriate mouse model.
Virology. 2018 08; 521:99-107.V

Abstract

Cases of Middle East respiratory syndrome coronavirus (MERS-CoV) continue to occur, making it one of the WHO´s targets for accelerated vaccine development. One vaccine candidate is based on live-attenuated measles virus (MV) vaccine encoding the MERS-CoV spike glycoprotein (MERS-S). MVvac2-MERS-S(H) induces robust humoral and cellular immunity against MERS-S mediating protection. Here, the induction and nature of immunity after vaccination with MVvac2-MERS-S(H) or novel MVvac2-MERS-N were further characterized. We focused on the necessity for vector replication and the nature of induced T cells, since functional CD8+ T cells contribute importantly to clearance of MERS-CoV. While no immunity against MERS-CoV or MV was detected in MV-susceptible mice after immunization with UV-inactivated virus, replication-competent MVvac2-MERS-S(H) triggered robust neutralizing antibody titers also in adult mice. Furthermore, a significant fraction of MERS CoV-specific CD8+ T cells and MV-specific CD4+ T cells simultaneously expressing IFN-γ and TNF-α were induced, revealing that MVvac2-MERS-S(H) induces multifunctional cellular immunity.

Authors+Show Affiliations

Product Testing of IVMPs, Div. of Veterinary Medicine, Paul-Ehrlich-Institut, Paul-Ehrlich-Str. 51-59, D-63225 Langen, Germany; German Center for Infection Research, Langen, Germany.Product Testing of IVMPs, Div. of Veterinary Medicine, Paul-Ehrlich-Institut, Paul-Ehrlich-Str. 51-59, D-63225 Langen, Germany; German Center for Infection Research, Langen, Germany.Product Testing of IVMPs, Div. of Veterinary Medicine, Paul-Ehrlich-Institut, Paul-Ehrlich-Str. 51-59, D-63225 Langen, Germany.Product Testing of IVMPs, Div. of Veterinary Medicine, Paul-Ehrlich-Institut, Paul-Ehrlich-Str. 51-59, D-63225 Langen, Germany.Product Testing of IVMPs, Div. of Veterinary Medicine, Paul-Ehrlich-Institut, Paul-Ehrlich-Str. 51-59, D-63225 Langen, Germany; German Center for Infection Research, Langen, Germany. Electronic address: Michael.Muehlebach@pei.de.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29902727

Citation

Bodmer, Bianca S., et al. "Live-attenuated Bivalent Measles Virus-derived Vaccines Targeting Middle East Respiratory Syndrome Coronavirus Induce Robust and Multifunctional T Cell Responses Against Both Viruses in an Appropriate Mouse Model." Virology, vol. 521, 2018, pp. 99-107.
Bodmer BS, Fiedler AH, Hanauer JRH, et al. Live-attenuated bivalent measles virus-derived vaccines targeting Middle East respiratory syndrome coronavirus induce robust and multifunctional T cell responses against both viruses in an appropriate mouse model. Virology. 2018;521:99-107.
Bodmer, B. S., Fiedler, A. H., Hanauer, J. R. H., Prüfer, S., & Mühlebach, M. D. (2018). Live-attenuated bivalent measles virus-derived vaccines targeting Middle East respiratory syndrome coronavirus induce robust and multifunctional T cell responses against both viruses in an appropriate mouse model. Virology, 521, 99-107. https://doi.org/10.1016/j.virol.2018.05.028
Bodmer BS, et al. Live-attenuated Bivalent Measles Virus-derived Vaccines Targeting Middle East Respiratory Syndrome Coronavirus Induce Robust and Multifunctional T Cell Responses Against Both Viruses in an Appropriate Mouse Model. Virology. 2018;521:99-107. PubMed PMID: 29902727.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Live-attenuated bivalent measles virus-derived vaccines targeting Middle East respiratory syndrome coronavirus induce robust and multifunctional T cell responses against both viruses in an appropriate mouse model. AU - Bodmer,Bianca S, AU - Fiedler,Anna H, AU - Hanauer,Jan R H, AU - Prüfer,Steffen, AU - Mühlebach,Michael D, Y1 - 2018/06/11/ PY - 2018/02/16/received PY - 2018/05/04/revised PY - 2018/05/31/accepted PY - 2018/6/15/pubmed PY - 2019/4/20/medline PY - 2018/6/15/entrez KW - Antibody responses KW - MERS Coronavirus KW - Measles Virus KW - Multifunctional T cells KW - Vaccine platform SP - 99 EP - 107 JF - Virology JO - Virology VL - 521 N2 - Cases of Middle East respiratory syndrome coronavirus (MERS-CoV) continue to occur, making it one of the WHO´s targets for accelerated vaccine development. One vaccine candidate is based on live-attenuated measles virus (MV) vaccine encoding the MERS-CoV spike glycoprotein (MERS-S). MVvac2-MERS-S(H) induces robust humoral and cellular immunity against MERS-S mediating protection. Here, the induction and nature of immunity after vaccination with MVvac2-MERS-S(H) or novel MVvac2-MERS-N were further characterized. We focused on the necessity for vector replication and the nature of induced T cells, since functional CD8+ T cells contribute importantly to clearance of MERS-CoV. While no immunity against MERS-CoV or MV was detected in MV-susceptible mice after immunization with UV-inactivated virus, replication-competent MVvac2-MERS-S(H) triggered robust neutralizing antibody titers also in adult mice. Furthermore, a significant fraction of MERS CoV-specific CD8+ T cells and MV-specific CD4+ T cells simultaneously expressing IFN-γ and TNF-α were induced, revealing that MVvac2-MERS-S(H) induces multifunctional cellular immunity. SN - 1096-0341 UR - https://www.unboundmedicine.com/medline/citation/29902727/full_citation L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(18)30169-7 DB - PRIME DP - Unbound Medicine ER -