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Genome-wide analysis of maize CONSTANS-LIKE gene family and expression profiling under light/dark and abscisic acid treatment.
Gene. 2018 Oct 05; 673:1-11.GENE

Abstract

The CONSTANS-LIKE (COL) gene has an important role both in regulation flowering through photoperiodic control and response to abiotic stress. In the present study, we performed a genome-wide analysis of maize COL gene family and identified 19 non-redundant ZmCOL genes, which were unequally distributed on ten maize chromosomes. Analysis of compound phylogenetic tree (maize, sorghum, rice and Arabidopsis) showed high bootstrap, as well as conserved domain and semblable gene structures among members within the same clade, revealing that COL genes in same clade were from the common ancestral and prior to the divergence of monocots and dicots lineages. Calculation of Ka/Ks ratio demonstrated that most duplicated ZmCOLs experienced purifying selection, which suggested limited functional divergence after duplication events. Comparing interspecies gene collinearity between three major grasses species, extensive microsynteny was detected among maize, sorghum and rice COL-containing segments. Additionally, several light-responsive and one ABA-responsive cis-elements could be detected for ZmCOL genes in group A. Therefore, qRT-PCR was performed to explore the expression patterns of ZmCOL genes in group A under light/dark conditions and ABA treatment. Our results laid the foundation for functional characterization of ZmCOL proteins and uncovering the biological roles of COL genes in response to stress in future.

Authors+Show Affiliations

National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei 230036, China.National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei 230036, China.National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei 230036, China.National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei 230036, China.National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei 230036, China.National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei 230036, China. Electronic address: weinasi@ahau.edu.cn.National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei 230036, China. Electronic address: lixiaoyuahau@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29908279

Citation

Song, Nannan, et al. "Genome-wide Analysis of Maize CONSTANS-LIKE Gene Family and Expression Profiling Under Light/dark and Abscisic Acid Treatment." Gene, vol. 673, 2018, pp. 1-11.
Song N, Xu Z, Wang J, et al. Genome-wide analysis of maize CONSTANS-LIKE gene family and expression profiling under light/dark and abscisic acid treatment. Gene. 2018;673:1-11.
Song, N., Xu, Z., Wang, J., Qin, Q., Jiang, H., Si, W., & Li, X. (2018). Genome-wide analysis of maize CONSTANS-LIKE gene family and expression profiling under light/dark and abscisic acid treatment. Gene, 673, 1-11. https://doi.org/10.1016/j.gene.2018.06.032
Song N, et al. Genome-wide Analysis of Maize CONSTANS-LIKE Gene Family and Expression Profiling Under Light/dark and Abscisic Acid Treatment. Gene. 2018 Oct 5;673:1-11. PubMed PMID: 29908279.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide analysis of maize CONSTANS-LIKE gene family and expression profiling under light/dark and abscisic acid treatment. AU - Song,Nannan, AU - Xu,Zhilan, AU - Wang,Jing, AU - Qin,Qianqian, AU - Jiang,Haiyang, AU - Si,Weina, AU - Li,Xiaoyu, Y1 - 2018/06/13/ PY - 2018/03/15/received PY - 2018/06/05/revised PY - 2018/06/11/accepted PY - 2018/6/17/pubmed PY - 2018/8/21/medline PY - 2018/6/17/entrez KW - ABA stress KW - CONSTANS-LIKE gene (COL) KW - Genome-wide analysis KW - Light regulation KW - Maize KW - Microsynteny analysis SP - 1 EP - 11 JF - Gene JO - Gene VL - 673 N2 - The CONSTANS-LIKE (COL) gene has an important role both in regulation flowering through photoperiodic control and response to abiotic stress. In the present study, we performed a genome-wide analysis of maize COL gene family and identified 19 non-redundant ZmCOL genes, which were unequally distributed on ten maize chromosomes. Analysis of compound phylogenetic tree (maize, sorghum, rice and Arabidopsis) showed high bootstrap, as well as conserved domain and semblable gene structures among members within the same clade, revealing that COL genes in same clade were from the common ancestral and prior to the divergence of monocots and dicots lineages. Calculation of Ka/Ks ratio demonstrated that most duplicated ZmCOLs experienced purifying selection, which suggested limited functional divergence after duplication events. Comparing interspecies gene collinearity between three major grasses species, extensive microsynteny was detected among maize, sorghum and rice COL-containing segments. Additionally, several light-responsive and one ABA-responsive cis-elements could be detected for ZmCOL genes in group A. Therefore, qRT-PCR was performed to explore the expression patterns of ZmCOL genes in group A under light/dark conditions and ABA treatment. Our results laid the foundation for functional characterization of ZmCOL proteins and uncovering the biological roles of COL genes in response to stress in future. SN - 1879-0038 UR - https://www.unboundmedicine.com/medline/citation/29908279/Genome_wide_analysis_of_maize_CONSTANS_LIKE_gene_family_and_expression_profiling_under_light/dark_and_abscisic_acid_treatment_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(18)30683-8 DB - PRIME DP - Unbound Medicine ER -