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Kuijieling regulates the differentiation of Treg and Th17 cells to ameliorate experimental colitis in rats.
Biomed Pharmacother 2018; 105:781-788BP

Abstract

BACKGROUND

Regulatory T (Treg) cells and T helper 17 (Th17) cells play crucial roles in ulcerative colitis (UC). Kuijieling (KJL) is an effective Chinese medicine formula for treating UC in clinic. Kuijieling has shown remedy effect on the imbalance between Treg and Th17 cells. This study aimed to further reveal the exact underlying mechanism of how Kuijieling regulates the differentiation of Treg and Th17 cells in the treatment of UC.

METHODS

Colitis was induced by trinitrobenzene sulfonic acid in rats and treated by KJL. Pathological injury was evaluated by HE staining and pathological score. Transforming growth factor-β1 (TGF-β1), interleukin(IL)-2, IL-6, IL-10, IL-17, IL-23 and IL-21 in plasma were assayed by ELISA. Forkhead box P3 (Foxp3), signal transducer and activator of transcription (STAT) 5 expressed in colon mucosa were measured by western blot. Immunohistochemistry was employed for quantifying retinoic acid-related orphan receptor γt (RORγt) and STAT3 in colon. RT-PCR was used to analyze the expression of IL-2, IL-17, IL-23, IL-21 mRNA in colon.

RESULTS

After the administration of KJL, pathological injury in colon mucosa was reduced and histological score was decreased, transforming growth factor-β1 (TGF-β1), interleukin(IL)-2, IL-10 in blood and Foxp3, STAT5, IL-2 in colon increased significantly, IL-6, IL-23, IL-17, IL-21 in blood and RORγt, STAT3, IL-23, IL-17, IL-21 in colon decreased. Our result showed that KJL regulates the related cytokines and transcription factors to promote Treg cells and suppress Th17 cells.

CONCLUSION

KJL restores the balance between Treg and Th17 cells through regulating the differentiation of them, therefore contributes to the treatment of UC.

Authors+Show Affiliations

Pi-Wei Institute, Guangzhou University of Chinese Medicine, China. Electronic address: 512351988@qq.com.Pi-Wei Institute, Guangzhou University of Chinese Medicine, China. Electronic address: 592108071@qq.com.Pi-Wei Institute, Guangzhou University of Chinese Medicine, China. Electronic address: 535586207@qq.com.State Key Laboratory of Applied Microbiology in Southern China, Guangdong Institute of Microbiology, China. Electronic address: liweixie1984@gmail.com.Pi-Wei Institute, Guangzhou University of Chinese Medicine, China. Electronic address: 947114556@qq.com.Pi-Wei Institute, Guangzhou University of Chinese Medicine, China. Electronic address: 466128018@qq.com.Pi-Wei Institute, Guangzhou University of Chinese Medicine, China. Electronic address: 563449098@qq.com.Pi-Wei Institute, Guangzhou University of Chinese Medicine, China. Electronic address: lyw_7614@126.com.Pi-Wei Institute, Guangzhou University of Chinese Medicine, China. Electronic address: duqun@gzucm.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29909346

Citation

Long, Yu, et al. "Kuijieling Regulates the Differentiation of Treg and Th17 Cells to Ameliorate Experimental Colitis in Rats." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 105, 2018, pp. 781-788.
Long Y, Li S, Qin J, et al. Kuijieling regulates the differentiation of Treg and Th17 cells to ameliorate experimental colitis in rats. Biomed Pharmacother. 2018;105:781-788.
Long, Y., Li, S., Qin, J., Xie, L., Gan, L., Jie, F., ... Du, Q. (2018). Kuijieling regulates the differentiation of Treg and Th17 cells to ameliorate experimental colitis in rats. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 105, pp. 781-788. doi:10.1016/j.biopha.2018.06.011.
Long Y, et al. Kuijieling Regulates the Differentiation of Treg and Th17 Cells to Ameliorate Experimental Colitis in Rats. Biomed Pharmacother. 2018;105:781-788. PubMed PMID: 29909346.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Kuijieling regulates the differentiation of Treg and Th17 cells to ameliorate experimental colitis in rats. AU - Long,Yu, AU - Li,Sixin, AU - Qin,Jingchun, AU - Xie,Liwei, AU - Gan,Liping, AU - Jie,Fengming, AU - Wu,Yanli, AU - Li,Yanwu, AU - Du,Qun, Y1 - 2018/06/15/ PY - 2017/12/22/received PY - 2018/06/01/revised PY - 2018/06/02/accepted PY - 2018/6/18/pubmed PY - 2018/11/16/medline PY - 2018/6/18/entrez KW - Atractylodes macrocephala KW - Chinese medicine KW - Forkhead box P3 KW - Ilex rotunda Thunb KW - Inflammatory bowel disease KW - Interleukin-10 KW - Interleukin-17 KW - Interleukin-2 KW - Interleukin-21 KW - Interleukin-23 KW - Interleukin-6 KW - Kuijieling KW - Paeonia lactiflora pall KW - Regulatory T cell KW - Retinoic acid-related orphan receptor γt KW - Signal transducer and activator of transcription 3 KW - Signal transducer and activator of transcription 5 KW - T helper-17 cell KW - Transforming growth factor-β1 (TGF-β1) KW - Ulcerative colitis KW - Whitmania pigra Whitman KW - radix Glycyrrhizae Preparata SP - 781 EP - 788 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed. Pharmacother. VL - 105 N2 - BACKGROUND: Regulatory T (Treg) cells and T helper 17 (Th17) cells play crucial roles in ulcerative colitis (UC). Kuijieling (KJL) is an effective Chinese medicine formula for treating UC in clinic. Kuijieling has shown remedy effect on the imbalance between Treg and Th17 cells. This study aimed to further reveal the exact underlying mechanism of how Kuijieling regulates the differentiation of Treg and Th17 cells in the treatment of UC. METHODS: Colitis was induced by trinitrobenzene sulfonic acid in rats and treated by KJL. Pathological injury was evaluated by HE staining and pathological score. Transforming growth factor-β1 (TGF-β1), interleukin(IL)-2, IL-6, IL-10, IL-17, IL-23 and IL-21 in plasma were assayed by ELISA. Forkhead box P3 (Foxp3), signal transducer and activator of transcription (STAT) 5 expressed in colon mucosa were measured by western blot. Immunohistochemistry was employed for quantifying retinoic acid-related orphan receptor γt (RORγt) and STAT3 in colon. RT-PCR was used to analyze the expression of IL-2, IL-17, IL-23, IL-21 mRNA in colon. RESULTS: After the administration of KJL, pathological injury in colon mucosa was reduced and histological score was decreased, transforming growth factor-β1 (TGF-β1), interleukin(IL)-2, IL-10 in blood and Foxp3, STAT5, IL-2 in colon increased significantly, IL-6, IL-23, IL-17, IL-21 in blood and RORγt, STAT3, IL-23, IL-17, IL-21 in colon decreased. Our result showed that KJL regulates the related cytokines and transcription factors to promote Treg cells and suppress Th17 cells. CONCLUSION: KJL restores the balance between Treg and Th17 cells through regulating the differentiation of them, therefore contributes to the treatment of UC. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/29909346/Kuijieling_regulates_the_differentiation_of_Treg_and_Th17_cells_to_ameliorate_experimental_colitis_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0753-3322(17)37029-4 DB - PRIME DP - Unbound Medicine ER -