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Cutting Edge Genetics: CRISPR/Cas9 Editing of Plant Genomes.
Plant Cell Physiol. 2018 Aug 01; 59(8):1608-1620.PC

Abstract

The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated nuclease 9 (Cas9) system is a genome editing technology transforming the field of plant biology by virtue of the system's efficiency and specificity. The system has quickly evolved for many diverse applications including multiplex gene mutation, gene replacement and transcriptional control. As CRISPR/Cas9 is increasingly applied to plants, it is becoming clear that each component of the system can be modified to improve editing results. This review aims to highlight common considerations and options when conducting CRISPR/Cas9 experiments.

Authors+Show Affiliations

Department of Biology and Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, 4155 Genome Sciences Building, 250 Bell Tower Drive, Chapel Hill, NC, USA.Department of Biology and Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, 4155 Genome Sciences Building, 250 Bell Tower Drive, Chapel Hill, NC, USA.Department of Biology and Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, 4155 Genome Sciences Building, 250 Bell Tower Drive, Chapel Hill, NC, USA.Department of Biology and Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, 4155 Genome Sciences Building, 250 Bell Tower Drive, Chapel Hill, NC, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29912402

Citation

Soyars, Cara L., et al. "Cutting Edge Genetics: CRISPR/Cas9 Editing of Plant Genomes." Plant & Cell Physiology, vol. 59, no. 8, 2018, pp. 1608-1620.
Soyars CL, Peterson BA, Burr CA, et al. Cutting Edge Genetics: CRISPR/Cas9 Editing of Plant Genomes. Plant Cell Physiol. 2018;59(8):1608-1620.
Soyars, C. L., Peterson, B. A., Burr, C. A., & Nimchuk, Z. L. (2018). Cutting Edge Genetics: CRISPR/Cas9 Editing of Plant Genomes. Plant & Cell Physiology, 59(8), 1608-1620. https://doi.org/10.1093/pcp/pcy079
Soyars CL, et al. Cutting Edge Genetics: CRISPR/Cas9 Editing of Plant Genomes. Plant Cell Physiol. 2018 Aug 1;59(8):1608-1620. PubMed PMID: 29912402.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cutting Edge Genetics: CRISPR/Cas9 Editing of Plant Genomes. AU - Soyars,Cara L, AU - Peterson,Brenda A, AU - Burr,Christian A, AU - Nimchuk,Zachary L, PY - 2017/11/15/received PY - 2018/04/11/accepted PY - 2018/6/19/pubmed PY - 2018/10/23/medline PY - 2018/6/19/entrez SP - 1608 EP - 1620 JF - Plant & cell physiology JO - Plant Cell Physiol. VL - 59 IS - 8 N2 - The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated nuclease 9 (Cas9) system is a genome editing technology transforming the field of plant biology by virtue of the system's efficiency and specificity. The system has quickly evolved for many diverse applications including multiplex gene mutation, gene replacement and transcriptional control. As CRISPR/Cas9 is increasingly applied to plants, it is becoming clear that each component of the system can be modified to improve editing results. This review aims to highlight common considerations and options when conducting CRISPR/Cas9 experiments. SN - 1471-9053 UR - https://www.unboundmedicine.com/medline/citation/29912402/Cutting_Edge_Genetics:_CRISPR/Cas9_Editing_of_Plant_Genomes_ L2 - https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcy079 DB - PRIME DP - Unbound Medicine ER -