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Protective effect of tadalafil and verapamil on testicular function and oxidative stress after torsion/detorsion in adult male rat.
Andrologia. 2018 Oct; 50(8):e13068.A

Abstract

OBJECTIVE

This study used a rat model to investigate the protective effect of tadalafil and verapamil on testicular function and oxidative stress after torsion/detorsion.

METHODS AND MATERIALS

Animals were randomly divided into five groups: G1, Sham group; G2, testicular torsion followed by detorsion (TD); G3, testicular torsion/detorsion received 0/4 mg/kg of tadalafil (TDT); G4, testicular torsion/detorsion received 0/1 mg/kg of verapamil (TDV); and G5, testicular torsion/detorsion received 0/1 mg/kg of verapamil and 0/4 mg/kg of tadalafil (TDTV). All treated groups were received the treatment 30 min before detorsion. Also, after reperfusion period (24 hr), the parameters of spermatozoa were assayed, and blood was measured for oxidative stress markers such as superoxide dismutase (SOD), glutathione peroxidase (GPx), activity of malondialdehyde (MDA) and blood levels of testosterone. The histological parameters investigated by Johnson's scores (JS), and also the seminiferous tubule diameter (STD) and the height of the germinal epithelium (HE) were measured using the linear eyepiece grids on the light microscope.

RESULTS

Between Sham and other groups were observed a significant change in histological parameters. Also, the levels of SOD, GPx and testosterone hormone were significantly decreased in TD while these increased in therapeutic groups. In the duration of ischaemia, the MDA level increased. Treatment with tadalafil and verapamil decreased the MDA level in treatment groups and also observed a significant change in sperm parameters between Sham and other groups.

CONCLUSIONS

Tadalafil and verapamil can be protected the testis tissue damage and replaced the testicular function by suppressing oxidative stress after testicular torsion.

Authors+Show Affiliations

Department of clinical Sciences, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran.Department of clinical Sciences, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran.Department of Basic Sciences, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran.Department of Anatomical Sciences and Embryology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29917246

Citation

Ameli, Mojtaba, et al. "Protective Effect of Tadalafil and Verapamil On Testicular Function and Oxidative Stress After Torsion/detorsion in Adult Male Rat." Andrologia, vol. 50, no. 8, 2018, pp. e13068.
Ameli M, Hashemi MS, Moghimian M, et al. Protective effect of tadalafil and verapamil on testicular function and oxidative stress after torsion/detorsion in adult male rat. Andrologia. 2018;50(8):e13068.
Ameli, M., Hashemi, M. S., Moghimian, M., & Shokoohi, M. (2018). Protective effect of tadalafil and verapamil on testicular function and oxidative stress after torsion/detorsion in adult male rat. Andrologia, 50(8), e13068. https://doi.org/10.1111/and.13068
Ameli M, et al. Protective Effect of Tadalafil and Verapamil On Testicular Function and Oxidative Stress After Torsion/detorsion in Adult Male Rat. Andrologia. 2018;50(8):e13068. PubMed PMID: 29917246.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of tadalafil and verapamil on testicular function and oxidative stress after torsion/detorsion in adult male rat. AU - Ameli,Mojtaba, AU - Hashemi,Mohaddeseh Sadat, AU - Moghimian,Maryam, AU - Shokoohi,Majid, Y1 - 2018/06/19/ PY - 2017/12/15/received PY - 2018/03/15/revised PY - 2018/03/19/accepted PY - 2018/6/20/pubmed PY - 2019/1/15/medline PY - 2018/6/20/entrez KW - oxidative stress KW - tadalafil KW - testicular function KW - torsion/detorsion KW - verapamil SP - e13068 EP - e13068 JF - Andrologia JO - Andrologia VL - 50 IS - 8 N2 - OBJECTIVE: This study used a rat model to investigate the protective effect of tadalafil and verapamil on testicular function and oxidative stress after torsion/detorsion. METHODS AND MATERIALS: Animals were randomly divided into five groups: G1, Sham group; G2, testicular torsion followed by detorsion (TD); G3, testicular torsion/detorsion received 0/4 mg/kg of tadalafil (TDT); G4, testicular torsion/detorsion received 0/1 mg/kg of verapamil (TDV); and G5, testicular torsion/detorsion received 0/1 mg/kg of verapamil and 0/4 mg/kg of tadalafil (TDTV). All treated groups were received the treatment 30 min before detorsion. Also, after reperfusion period (24 hr), the parameters of spermatozoa were assayed, and blood was measured for oxidative stress markers such as superoxide dismutase (SOD), glutathione peroxidase (GPx), activity of malondialdehyde (MDA) and blood levels of testosterone. The histological parameters investigated by Johnson's scores (JS), and also the seminiferous tubule diameter (STD) and the height of the germinal epithelium (HE) were measured using the linear eyepiece grids on the light microscope. RESULTS: Between Sham and other groups were observed a significant change in histological parameters. Also, the levels of SOD, GPx and testosterone hormone were significantly decreased in TD while these increased in therapeutic groups. In the duration of ischaemia, the MDA level increased. Treatment with tadalafil and verapamil decreased the MDA level in treatment groups and also observed a significant change in sperm parameters between Sham and other groups. CONCLUSIONS: Tadalafil and verapamil can be protected the testis tissue damage and replaced the testicular function by suppressing oxidative stress after testicular torsion. SN - 1439-0272 UR - https://www.unboundmedicine.com/medline/citation/29917246/Protective_effect_of_tadalafil_and_verapamil_on_testicular_function_and_oxidative_stress_after_torsion/detorsion_in_adult_male_rat_ L2 - https://doi.org/10.1111/and.13068 DB - PRIME DP - Unbound Medicine ER -