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Quantitative proteomic analysis and antivenom study revealing that neurotoxic phospholipase A2 enzymes, the major toxin class of Russell's viper venom from southern India, shows the least immuno-recognition and neutralization by commercial polyvalent antivenom.
Int J Biol Macromol. 2018 Oct 15; 118(Pt A):375-385.IJ

Abstract

The proteome composition of Russell's viper venom (RVV) from southern India (SI) was investigated by 1D-SDS-PAGE of venom followed by tandem mass spectrometry analysis of protein bands. A total of 66 proteins belonging to 14 snake venom protein families were identified by LC-MS/MS analysis against Viperidae (taxid 8689) protein entries from the non-redundant NCBI database. Phospholipase A2 (43.25%) and snaclec (14.57%) represented the most abundant enzymatic and non-enzymatic proteins, respectively. SI RVV was characterized as containing a higher quantity of PLA2 and a lower amount of Kunitz-type serine protease inhibitors, in comparison to RVV from other regions of the Indian subcontinent. The enzymatic activities, pharmacological properties, and clinical manifestations of RV envenomation in SI were well correlated with its proteome composition; however, ATPase, ADPase, and hyaluronidase enzymes were not identified by LC-MS/MS analysis, owing to paucity of the existing database. Neurological symptoms exhibited by RV-bite patients in SI were correlated to the presence of abundant neurotoxic phospholipase A2 enzymes (15.66%) in SI RVV. Neutralization studies, immunological cross-reactivity, and antivenomics studies unequivocally demonstrated the poor recognition and lowest neutralization of PLA2 enzymes by commercial polyvalent antivenom, which is a major concern for the treatment of RV-envenomed patients in SI.

Authors+Show Affiliations

Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India. Electronic address: akm@tezu.ernet.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29924981

Citation

Kalita, Bhargab, et al. "Quantitative Proteomic Analysis and Antivenom Study Revealing That Neurotoxic Phospholipase A2 Enzymes, the Major Toxin Class of Russell's Viper Venom From Southern India, Shows the Least Immuno-recognition and Neutralization By Commercial Polyvalent Antivenom." International Journal of Biological Macromolecules, vol. 118, no. Pt A, 2018, pp. 375-385.
Kalita B, Singh S, Patra A, et al. Quantitative proteomic analysis and antivenom study revealing that neurotoxic phospholipase A2 enzymes, the major toxin class of Russell's viper venom from southern India, shows the least immuno-recognition and neutralization by commercial polyvalent antivenom. Int J Biol Macromol. 2018;118(Pt A):375-385.
Kalita, B., Singh, S., Patra, A., & Mukherjee, A. K. (2018). Quantitative proteomic analysis and antivenom study revealing that neurotoxic phospholipase A2 enzymes, the major toxin class of Russell's viper venom from southern India, shows the least immuno-recognition and neutralization by commercial polyvalent antivenom. International Journal of Biological Macromolecules, 118(Pt A), 375-385. https://doi.org/10.1016/j.ijbiomac.2018.06.083
Kalita B, et al. Quantitative Proteomic Analysis and Antivenom Study Revealing That Neurotoxic Phospholipase A2 Enzymes, the Major Toxin Class of Russell's Viper Venom From Southern India, Shows the Least Immuno-recognition and Neutralization By Commercial Polyvalent Antivenom. Int J Biol Macromol. 2018 Oct 15;118(Pt A):375-385. PubMed PMID: 29924981.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative proteomic analysis and antivenom study revealing that neurotoxic phospholipase A2 enzymes, the major toxin class of Russell's viper venom from southern India, shows the least immuno-recognition and neutralization by commercial polyvalent antivenom. AU - Kalita,Bhargab, AU - Singh,Sudeepa, AU - Patra,Aparup, AU - Mukherjee,Ashis K, Y1 - 2018/06/18/ PY - 2018/04/08/received PY - 2018/06/14/revised PY - 2018/06/15/accepted PY - 2018/6/21/pubmed PY - 2018/12/12/medline PY - 2018/6/21/entrez KW - Antivenomics KW - ESI-LC-MS/MS KW - Neurotoxic KW - Pro-coagulant KW - Snake venom KW - Viperidae SP - 375 EP - 385 JF - International journal of biological macromolecules JO - Int. J. Biol. Macromol. VL - 118 IS - Pt A N2 - The proteome composition of Russell's viper venom (RVV) from southern India (SI) was investigated by 1D-SDS-PAGE of venom followed by tandem mass spectrometry analysis of protein bands. A total of 66 proteins belonging to 14 snake venom protein families were identified by LC-MS/MS analysis against Viperidae (taxid 8689) protein entries from the non-redundant NCBI database. Phospholipase A2 (43.25%) and snaclec (14.57%) represented the most abundant enzymatic and non-enzymatic proteins, respectively. SI RVV was characterized as containing a higher quantity of PLA2 and a lower amount of Kunitz-type serine protease inhibitors, in comparison to RVV from other regions of the Indian subcontinent. The enzymatic activities, pharmacological properties, and clinical manifestations of RV envenomation in SI were well correlated with its proteome composition; however, ATPase, ADPase, and hyaluronidase enzymes were not identified by LC-MS/MS analysis, owing to paucity of the existing database. Neurological symptoms exhibited by RV-bite patients in SI were correlated to the presence of abundant neurotoxic phospholipase A2 enzymes (15.66%) in SI RVV. Neutralization studies, immunological cross-reactivity, and antivenomics studies unequivocally demonstrated the poor recognition and lowest neutralization of PLA2 enzymes by commercial polyvalent antivenom, which is a major concern for the treatment of RV-envenomed patients in SI. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/29924981/Quantitative_proteomic_analysis_and_antivenom_study_revealing_that_neurotoxic_phospholipase_A2_enzymes_the_major_toxin_class_of_Russell's_viper_venom_from_southern_India_shows_the_least_immuno_recognition_and_neutralization_by_commercial_polyvalent_antivenom_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(18)31644-1 DB - PRIME DP - Unbound Medicine ER -