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The long periodicity phase (LPP) controversy part I: The influence of a natural-like ratio of the CER[EOS] analogue [EOS]-br in a CER[NP]/[AP] based stratum corneum modelling system: A neutron diffraction study.
Biochim Biophys Acta Biomembr. 2019 01; 1861(1):306-315.BB

Abstract

This study used neutron diffraction to investigate a ceramide-[NP] C24/[AP] C24 /[EOS]-br C30/cholesterol/lignoceric acid (0.6: 0.3: 0.1: 0.7: 1) based stratum corneum modelling system. By adding specifically deuterated ceramides-[NP]-D3, [AP]-D3, and [EOS]-br-D3, detailed information on the lamellar and the nanostructure of the system was obtained. For the short periodicity phase a natural-like lamellar repeat distance of 5.47 ± 0.02 nm was observed, similar to the [NP]/[AP] base system without the [EOS]-br. Unlike in this system the ceramides here were slightly tilted, hinting towards a slightly less natural arrangement. Due to the deuteration it was possible to observe that the long ceramide chains were overlapping in the lamellar mid-plane. This is considered to be an important feature for the natural stratum corneum. Despite the presence of a ceramide [EOS] analogue - able to form a long phase arrangement - no distinct long periodicity phase was formed, despite a slightly higher than natural ω-acyl ceramide ratio of 10 mol%. The deuterated variant of this ceramide determined that the very long ceramide was integrated into the short periodicity phase, spanning multiple layers instead. The - compared to the base system - unchanged repeat distance highlights the stability of this structure. Furthermore, the localisation of the very long ceramide in the short periodicity phase indicates the possibility of a crosslinking effect and thus a multilayer stabilizing role for the ceramide [EOS]. It can be concluded, that additionally to the mere presence of ceramide-[EOS] more complex conditions have to be met in order to form this long phase. This has to be further investigated in the future.

Authors+Show Affiliations

Institute of Applied Dermatopharmacy at the Martin Luther University Halle-Wittenberg (IADP), Weinbergweg 23, 06120 Halle/Saale, Germany.Institute of Medical Physics and Biophysics, University of Leipzig, Härtelstraβe 16-18, 04107 Leipzig, Germany; Institute of Pharmacy, Martin Luther University Halle-Wittenberg (MLU), Wolfgang-Langenbeck-Str. 4, 06120 Halle/Saale, Germany.Institute of Pharmacy, Martin Luther University Halle-Wittenberg (MLU), Wolfgang-Langenbeck-Str. 4, 06120 Halle/Saale, Germany.Institute of Pharmacy, Martin Luther University Halle-Wittenberg (MLU), Wolfgang-Langenbeck-Str. 4, 06120 Halle/Saale, Germany.Institut Laue-Langevin, 71 avenue des Martyrs, CS 20156, 38042 Grenoble, CEDEX 9, France.Institute of Pharmacy, Martin Luther University Halle-Wittenberg (MLU), Wolfgang-Langenbeck-Str. 4, 06120 Halle/Saale, Germany.Institute of Applied Dermatopharmacy at the Martin Luther University Halle-Wittenberg (IADP), Weinbergweg 23, 06120 Halle/Saale, Germany; Institute of Pharmacy, Martin Luther University Halle-Wittenberg (MLU), Wolfgang-Langenbeck-Str. 4, 06120 Halle/Saale, Germany. Electronic address: reinhard.neubert@pharmazie.uni-halle.de.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29924985

Citation

Schmitt, Thomas, et al. "The Long Periodicity Phase (LPP) Controversy Part I: the Influence of a Natural-like Ratio of the CER[EOS] Analogue [EOS]-br in a CER[NP]/[AP] Based Stratum Corneum Modelling System: a Neutron Diffraction Study." Biochimica Et Biophysica Acta. Biomembranes, vol. 1861, no. 1, 2019, pp. 306-315.
Schmitt T, Lange S, Sonnenberger S, et al. The long periodicity phase (LPP) controversy part I: The influence of a natural-like ratio of the CER[EOS] analogue [EOS]-br in a CER[NP]/[AP] based stratum corneum modelling system: A neutron diffraction study. Biochim Biophys Acta Biomembr. 2019;1861(1):306-315.
Schmitt, T., Lange, S., Sonnenberger, S., Dobner, B., Demé, B., Langner, A., & Neubert, R. H. H. (2019). The long periodicity phase (LPP) controversy part I: The influence of a natural-like ratio of the CER[EOS] analogue [EOS]-br in a CER[NP]/[AP] based stratum corneum modelling system: A neutron diffraction study. Biochimica Et Biophysica Acta. Biomembranes, 1861(1), 306-315. https://doi.org/10.1016/j.bbamem.2018.06.008
Schmitt T, et al. The Long Periodicity Phase (LPP) Controversy Part I: the Influence of a Natural-like Ratio of the CER[EOS] Analogue [EOS]-br in a CER[NP]/[AP] Based Stratum Corneum Modelling System: a Neutron Diffraction Study. Biochim Biophys Acta Biomembr. 2019;1861(1):306-315. PubMed PMID: 29924985.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The long periodicity phase (LPP) controversy part I: The influence of a natural-like ratio of the CER[EOS] analogue [EOS]-br in a CER[NP]/[AP] based stratum corneum modelling system: A neutron diffraction study. AU - Schmitt,Thomas, AU - Lange,Stefan, AU - Sonnenberger,Stefan, AU - Dobner,Bodo, AU - Demé,Bruno, AU - Langner,Andreas, AU - Neubert,Reinhard H H, Y1 - 2018/06/18/ PY - 2017/10/05/received PY - 2018/05/07/revised PY - 2018/06/11/accepted PY - 2018/6/21/pubmed PY - 2019/8/21/medline PY - 2018/6/21/entrez KW - CER[AP] KW - CER[EOS] KW - CER[NP] KW - LPP KW - Long periodicity phase KW - Neutron diffraction SP - 306 EP - 315 JF - Biochimica et biophysica acta. Biomembranes JO - Biochim Biophys Acta Biomembr VL - 1861 IS - 1 N2 - This study used neutron diffraction to investigate a ceramide-[NP] C24/[AP] C24 /[EOS]-br C30/cholesterol/lignoceric acid (0.6: 0.3: 0.1: 0.7: 1) based stratum corneum modelling system. By adding specifically deuterated ceramides-[NP]-D3, [AP]-D3, and [EOS]-br-D3, detailed information on the lamellar and the nanostructure of the system was obtained. For the short periodicity phase a natural-like lamellar repeat distance of 5.47 ± 0.02 nm was observed, similar to the [NP]/[AP] base system without the [EOS]-br. Unlike in this system the ceramides here were slightly tilted, hinting towards a slightly less natural arrangement. Due to the deuteration it was possible to observe that the long ceramide chains were overlapping in the lamellar mid-plane. This is considered to be an important feature for the natural stratum corneum. Despite the presence of a ceramide [EOS] analogue - able to form a long phase arrangement - no distinct long periodicity phase was formed, despite a slightly higher than natural ω-acyl ceramide ratio of 10 mol%. The deuterated variant of this ceramide determined that the very long ceramide was integrated into the short periodicity phase, spanning multiple layers instead. The - compared to the base system - unchanged repeat distance highlights the stability of this structure. Furthermore, the localisation of the very long ceramide in the short periodicity phase indicates the possibility of a crosslinking effect and thus a multilayer stabilizing role for the ceramide [EOS]. It can be concluded, that additionally to the mere presence of ceramide-[EOS] more complex conditions have to be met in order to form this long phase. This has to be further investigated in the future. SN - 1879-2642 UR - https://www.unboundmedicine.com/medline/citation/29924985/The_long_periodicity_phase__LPP__controversy_part_I:_The_influence_of_a_natural_like_ratio_of_the_CER[EOS]_analogue_[EOS]_br_in_a_CER[NP]/[AP]_based_stratum_corneum_modelling_system:_A_neutron_diffraction_study_ DB - PRIME DP - Unbound Medicine ER -