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miR-26a suppresses osteosarcoma migration and invasion by directly targeting HMGA1.
Oncol Lett. 2018 Jun; 15(6):8303-8310.OL

Abstract

Osteosarcoma (OS) is identified as the most commonly diagnosed malignant cancer of bone, and has approximately three million new cases annually. miR-26a plays an important role in the development of various types of cancer. We investigated whether miR-26a can regulate the migration and invasion of OS by targeting high-mobility group A1 HMGA1. Western blot analysis was used to identify the changes of protein levels. Reverse transcription-quantitative PCR was used to test expression levels of genes and miR-26a. Luciferase reporter assay was used to test the specific target gene of miR-26a. Transwell assay was employed to determine the migration and invasion of OS cell lines. In the present study, miRNA-26a was frequently downregulated in OS tissues and cells. Overexpression of miR-26a inhibited cell migration and invasion in vitro. In addition, miR-26a downregulated HMGA1 by targeting its 3'-UTR and knockdown of HMGA1 significantly suppressed the migration and invasion of two osteosarcoma cell lines in vitro. miR-26a suppressed the migration and invasion of OS cells by targeting HMGA1, suggesting that miR-26a/HMGA1 axis provides a new prospective therapeutic strategy for OS.

Authors+Show Affiliations

Department of Orthopedics, People's Hospital of Weifang, Weifang, Shandong 261000, P.R. China.Department of Medicine, The First People's Hospital of Jinan, Jinan, Shandong 250000, P.R. China.Department of Respiratory Medicine, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China.Department of Neurology, People's Hospital of Rizhao, Rizhao, Shandong 276800, P.R. China.Department of Medicine, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China.Department of Clinical Laboratory, People's Hospital of Rizhao, Rizhao, Shandong 276800, P.R. China.Department of Orthopedics, Qingdao Hiser Medical Center, Qingdao, Shandong 266033, P.R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29928320

Citation

Liu, Jianyong, et al. "MiR-26a Suppresses Osteosarcoma Migration and Invasion By Directly Targeting HMGA1." Oncology Letters, vol. 15, no. 6, 2018, pp. 8303-8310.
Liu J, Mi B, Wang Y, et al. MiR-26a suppresses osteosarcoma migration and invasion by directly targeting HMGA1. Oncol Lett. 2018;15(6):8303-8310.
Liu, J., Mi, B., Wang, Y., Shi, C., Mi, X., Lu, Y., & Yu, P. (2018). MiR-26a suppresses osteosarcoma migration and invasion by directly targeting HMGA1. Oncology Letters, 15(6), 8303-8310. https://doi.org/10.3892/ol.2018.8359
Liu J, et al. MiR-26a Suppresses Osteosarcoma Migration and Invasion By Directly Targeting HMGA1. Oncol Lett. 2018;15(6):8303-8310. PubMed PMID: 29928320.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - miR-26a suppresses osteosarcoma migration and invasion by directly targeting HMGA1. AU - Liu,Jianyong, AU - Mi,Bo, AU - Wang,Yi, AU - Shi,Chunling, AU - Mi,Xiufang, AU - Lu,Yingying, AU - Yu,Peilin, Y1 - 2018/03/28/ PY - 2017/09/15/received PY - 2018/02/28/accepted PY - 2018/6/22/entrez PY - 2018/6/22/pubmed PY - 2018/6/22/medline KW - HMGA1 KW - invasion KW - miR-26a KW - migration KW - osteosarcoma SP - 8303 EP - 8310 JF - Oncology letters JO - Oncol Lett VL - 15 IS - 6 N2 - Osteosarcoma (OS) is identified as the most commonly diagnosed malignant cancer of bone, and has approximately three million new cases annually. miR-26a plays an important role in the development of various types of cancer. We investigated whether miR-26a can regulate the migration and invasion of OS by targeting high-mobility group A1 HMGA1. Western blot analysis was used to identify the changes of protein levels. Reverse transcription-quantitative PCR was used to test expression levels of genes and miR-26a. Luciferase reporter assay was used to test the specific target gene of miR-26a. Transwell assay was employed to determine the migration and invasion of OS cell lines. In the present study, miRNA-26a was frequently downregulated in OS tissues and cells. Overexpression of miR-26a inhibited cell migration and invasion in vitro. In addition, miR-26a downregulated HMGA1 by targeting its 3'-UTR and knockdown of HMGA1 significantly suppressed the migration and invasion of two osteosarcoma cell lines in vitro. miR-26a suppressed the migration and invasion of OS cells by targeting HMGA1, suggesting that miR-26a/HMGA1 axis provides a new prospective therapeutic strategy for OS. SN - 1792-1074 UR - https://www.unboundmedicine.com/medline/citation/29928320/miR_26a_suppresses_osteosarcoma_migration_and_invasion_by_directly_targeting_HMGA1_ L2 - https://www.ingentaconnect.com/openurl?genre=article&issn=1792-1074&volume=15&issue=6&spage=8303&aulast=Liu DB - PRIME DP - Unbound Medicine ER -
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