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Halobacteriovorax isolated from marine water of the Adriatic sea, Italy, as an effective predator of Vibrio parahaemolyticus, non-O1/O139 V. cholerae, V. vulnificus.

Abstract

AIM

To detect marine Bdellovibrio and like organisms (BALOs) which are able to infect Vibrio parahaemolyticus from seawater of the Adriatic, Italy. To test, prey specificity and predation efficiency of our Halobacteriovorax isolate, named HBXCO1, towards 17 Vibrio and 7 non-Vibrio strains linked to the Adriatic sea, Italy.

METHODS AND RESULTS

Double layer agar plating technique was used to enumerate BALOs and to evaluate their prey specificity and predation efficiency. Transmission electron microscopy and 16S rRNA analysis were used to identify them. Means of BALOs counts ranged from 5·0 PFU per ml (March 2017) to 98·6 PFU per ml (August 2016). HBXCO1 had the ability to attack all tested prey strains of V. parahaemolyticus, Vibrio cholerae non-O1/O139 and Vibrio vulnificus, but it did not prey on non-Vibrio strains and V. alginolyticus under the tested conditions.

CONCLUSIONS

Bdellovibrio and like organisms capable of infecting pathogenic vibrios are naturally present in seawater of the Adriatic, Italy. Isolate HBXCO1 shows prey specificity preferentially for the Vibrio genus and high predatory efficiency towards a wide range of pathogenic strains.

SIGNIFICANCE AND IMPACT OF THE STUDY

The public impact of V. parahaemolyticus, non-O1/O139 V. cholerae and V. vulnificus in bivalves is relevant and current decontamination processes are not always effective. We believe that the predator HBXCO1 represents a potential candidate for the development of strategies of biocontrol of pathogenic vibrios in bivalves from harvesting to trade.

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  • Authors+Show Affiliations

    ,

    Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Laboratorio Nazionale di Riferimento per il controllo delle Contaminazioni Batteriche dei Molluschi Bivalvi Vivi, Ancona, Italy.

    ,

    Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Laboratorio Nazionale di Riferimento per il controllo delle Contaminazioni Batteriche dei Molluschi Bivalvi Vivi, Ancona, Italy.

    ,

    Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Laboratorio Nazionale di Riferimento per il controllo delle Contaminazioni Batteriche dei Molluschi Bivalvi Vivi, Ancona, Italy.

    ,

    Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Laboratorio Nazionale di Riferimento per il controllo delle Contaminazioni Batteriche dei Molluschi Bivalvi Vivi, Ancona, Italy.

    ,

    Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Laboratorio Nazionale di Riferimento per il controllo delle Contaminazioni Batteriche dei Molluschi Bivalvi Vivi, Ancona, Italy.

    ,

    Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Laboratorio Nazionale di Riferimento per il controllo delle Contaminazioni Batteriche dei Molluschi Bivalvi Vivi, Ancona, Italy.

    Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Laboratorio Nazionale di Riferimento per il controllo delle Contaminazioni Batteriche dei Molluschi Bivalvi Vivi, Ancona, Italy.

    Source

    Journal of applied microbiology 125:4 2018 Oct pg 1199-1207

    MeSH

    Animals
    Antibiosis
    Bivalvia
    Italy
    Proteobacteria
    Seawater
    Vibrio cholerae
    Vibrio parahaemolyticus
    Vibrio vulnificus
    Water Microbiology

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    29931749

    Citation

    Ottaviani, D, et al. "Halobacteriovorax Isolated From Marine Water of the Adriatic Sea, Italy, as an Effective Predator of Vibrio Parahaemolyticus, non-O1/O139 V. Cholerae, V. Vulnificus." Journal of Applied Microbiology, vol. 125, no. 4, 2018, pp. 1199-1207.
    Ottaviani D, Chierichetti S, Angelico G, et al. Halobacteriovorax isolated from marine water of the Adriatic sea, Italy, as an effective predator of Vibrio parahaemolyticus, non-O1/O139 V. cholerae, V. vulnificus. J Appl Microbiol. 2018;125(4):1199-1207.
    Ottaviani, D., Chierichetti, S., Angelico, G., Forte, C., Rocchegiani, E., Manuali, E., & Leoni, F. (2018). Halobacteriovorax isolated from marine water of the Adriatic sea, Italy, as an effective predator of Vibrio parahaemolyticus, non-O1/O139 V. cholerae, V. vulnificus. Journal of Applied Microbiology, 125(4), pp. 1199-1207. doi:10.1111/jam.14027.
    Ottaviani D, et al. Halobacteriovorax Isolated From Marine Water of the Adriatic Sea, Italy, as an Effective Predator of Vibrio Parahaemolyticus, non-O1/O139 V. Cholerae, V. Vulnificus. J Appl Microbiol. 2018;125(4):1199-1207. PubMed PMID: 29931749.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Halobacteriovorax isolated from marine water of the Adriatic sea, Italy, as an effective predator of Vibrio parahaemolyticus, non-O1/O139 V. cholerae, V. vulnificus. AU - Ottaviani,D, AU - Chierichetti,S, AU - Angelico,G, AU - Forte,C, AU - Rocchegiani,E, AU - Manuali,E, AU - Leoni,F, Y1 - 2018/09/02/ PY - 2017/11/10/received PY - 2018/06/04/revised PY - 2018/06/15/accepted PY - 2018/6/23/pubmed PY - 2018/11/27/medline PY - 2018/6/23/entrez KW - Halobacteriovorax KW - V. vulnificus KW - Vibrio parahaemolyticus KW - Bdellovibrio and like organisms KW - Vibrio-specific predator KW - non-O1/O139 V. cholerae SP - 1199 EP - 1207 JF - Journal of applied microbiology JO - J. Appl. Microbiol. VL - 125 IS - 4 N2 - AIM: To detect marine Bdellovibrio and like organisms (BALOs) which are able to infect Vibrio parahaemolyticus from seawater of the Adriatic, Italy. To test, prey specificity and predation efficiency of our Halobacteriovorax isolate, named HBXCO1, towards 17 Vibrio and 7 non-Vibrio strains linked to the Adriatic sea, Italy. METHODS AND RESULTS: Double layer agar plating technique was used to enumerate BALOs and to evaluate their prey specificity and predation efficiency. Transmission electron microscopy and 16S rRNA analysis were used to identify them. Means of BALOs counts ranged from 5·0 PFU per ml (March 2017) to 98·6 PFU per ml (August 2016). HBXCO1 had the ability to attack all tested prey strains of V. parahaemolyticus, Vibrio cholerae non-O1/O139 and Vibrio vulnificus, but it did not prey on non-Vibrio strains and V. alginolyticus under the tested conditions. CONCLUSIONS: Bdellovibrio and like organisms capable of infecting pathogenic vibrios are naturally present in seawater of the Adriatic, Italy. Isolate HBXCO1 shows prey specificity preferentially for the Vibrio genus and high predatory efficiency towards a wide range of pathogenic strains. SIGNIFICANCE AND IMPACT OF THE STUDY: The public impact of V. parahaemolyticus, non-O1/O139 V. cholerae and V. vulnificus in bivalves is relevant and current decontamination processes are not always effective. We believe that the predator HBXCO1 represents a potential candidate for the development of strategies of biocontrol of pathogenic vibrios in bivalves from harvesting to trade. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/29931749/Halobacteriovorax_isolated_from_marine_water_of_the_Adriatic_sea,_Italy,_as_an_effective_predator_of_Vibrio_parahaemolyticus,_non-O1/O139_V._cholerae,_V._vulnificus L2 - https://doi.org/10.1111/jam.14027 DB - PRIME DP - Unbound Medicine ER -