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Transcriptomics Investigation into the Mechanisms of Self-Incompatibility between Pin and Thrum Morphs of Primula maximowiczii.
Int J Mol Sci 2018; 19(7)IJ

Abstract

Heteromorphic self-incompatibility (SI) is an important system for preventing inbreeding in the genus Primula. However, investigations into the molecular mechanisms of Primula SI are lacking. To explore the mechanisms of SI in Primula maximowiczii, the pollen germination and fruiting rates of self- and cross-pollinations between pin and thrum morphs were investigated, and transcriptomics analyses of the pistils after pollination were performed to assess gene expression patterns in pin and thrum SI. The results indicated that P. maximowiczii exhibits strong SI and that the mechanisms of pollen tube inhibition differ between pin and thrum morphs. While self-pollen tubes of the pin morph were able to occasionally, though rarely, enter the style, those of the thrum morph were never observed to enter the style. The transcriptomics analysis of the pistils revealed 1311 and 1048 differentially expressed genes (DEGs) that were identified by comparing pin self-pollination (PS) vs. pin cross-pollination (PT) and thrum self-pollination (TS) vs. thrum cross-pollination (TP). Notably, about 90% of these DEGs exhibited different expression patterns in the two comparisons. Moreover, pin and thrum DEGs were associated with different Gene Ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways following enrichment analyses. Based on our results, the molecular mechanisms underlying the pin and thrum SI in P. maximowiczii appear to be distinct. Furthermore, the genes involved in the SI processes are commonly associated with carbohydrate metabolism and environmental adaptation. These results provide new insight into the molecular mechanisms of Primula SI.

Authors+Show Affiliations

Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education and College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China. wanplu@163.com.Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education and College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China. 13269895583@163.com. Development Center of Science and Technology, Ministry of Agriculture and Rural Affairs, Beijing 100083, China. 13269895583@163.com.Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education and College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China. yweiru@163.com.Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education and College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China. chengtangren@163.com.Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education and College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China. wangjia8248@163.com.Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education and College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China. zqxbjfu@126.com.Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education and College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China. htpan@bjfu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29932122

Citation

Lu, Wanpei, et al. "Transcriptomics Investigation Into the Mechanisms of Self-Incompatibility Between Pin and Thrum Morphs of Primula Maximowiczii." International Journal of Molecular Sciences, vol. 19, no. 7, 2018.
Lu W, Bian X, Yang W, et al. Transcriptomics Investigation into the Mechanisms of Self-Incompatibility between Pin and Thrum Morphs of Primula maximowiczii. Int J Mol Sci. 2018;19(7).
Lu, W., Bian, X., Yang, W., Cheng, T., Wang, J., Zhang, Q., & Pan, H. (2018). Transcriptomics Investigation into the Mechanisms of Self-Incompatibility between Pin and Thrum Morphs of Primula maximowiczii. International Journal of Molecular Sciences, 19(7), doi:10.3390/ijms19071840.
Lu W, et al. Transcriptomics Investigation Into the Mechanisms of Self-Incompatibility Between Pin and Thrum Morphs of Primula Maximowiczii. Int J Mol Sci. 2018 Jun 22;19(7) PubMed PMID: 29932122.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptomics Investigation into the Mechanisms of Self-Incompatibility between Pin and Thrum Morphs of Primula maximowiczii. AU - Lu,Wanpei, AU - Bian,Xiaomeng, AU - Yang,Weiru, AU - Cheng,Tangren, AU - Wang,Jia, AU - Zhang,Qixiang, AU - Pan,Huitang, Y1 - 2018/06/22/ PY - 2018/04/04/received PY - 2018/06/13/revised PY - 2018/06/17/accepted PY - 2018/6/23/entrez PY - 2018/6/23/pubmed PY - 2018/10/16/medline KW - Primula maximowiczii KW - differentially expressed genes KW - distyly KW - environmental adaptation KW - heteromorphic self-incompatibility JF - International journal of molecular sciences JO - Int J Mol Sci VL - 19 IS - 7 N2 - Heteromorphic self-incompatibility (SI) is an important system for preventing inbreeding in the genus Primula. However, investigations into the molecular mechanisms of Primula SI are lacking. To explore the mechanisms of SI in Primula maximowiczii, the pollen germination and fruiting rates of self- and cross-pollinations between pin and thrum morphs were investigated, and transcriptomics analyses of the pistils after pollination were performed to assess gene expression patterns in pin and thrum SI. The results indicated that P. maximowiczii exhibits strong SI and that the mechanisms of pollen tube inhibition differ between pin and thrum morphs. While self-pollen tubes of the pin morph were able to occasionally, though rarely, enter the style, those of the thrum morph were never observed to enter the style. The transcriptomics analysis of the pistils revealed 1311 and 1048 differentially expressed genes (DEGs) that were identified by comparing pin self-pollination (PS) vs. pin cross-pollination (PT) and thrum self-pollination (TS) vs. thrum cross-pollination (TP). Notably, about 90% of these DEGs exhibited different expression patterns in the two comparisons. Moreover, pin and thrum DEGs were associated with different Gene Ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways following enrichment analyses. Based on our results, the molecular mechanisms underlying the pin and thrum SI in P. maximowiczii appear to be distinct. Furthermore, the genes involved in the SI processes are commonly associated with carbohydrate metabolism and environmental adaptation. These results provide new insight into the molecular mechanisms of Primula SI. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/29932122/Transcriptomics_Investigation_into_the_Mechanisms_of_Self_Incompatibility_between_Pin_and_Thrum_Morphs_of_Primula_maximowiczii_ L2 - http://www.mdpi.com/resolver?pii=ijms19071840 DB - PRIME DP - Unbound Medicine ER -