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Identification of the merR gene of R100 by using mer-lac gene and operon fusions.
J Bacteriol 1985; 163(3):1153-7JB

Abstract

Transcriptional (operon) and translational (gene) fusions between the R100 merR gene and lacZ were constructed in vitro in a pBR322 plasmid carrying the mer genes derived from plasmid R100. The translational fusions were oriented in the opposite direction to and divergently from the merTCAD genes. This shows that the reading frame previously thought to be merR was incorrect. Expression of the gene fusion was repressed in trans by a compatible plasmid carrying the R100 merR+ gene, as was a similarly oriented transcriptional fusion. In contrast, expression of beta-galactosidase by the lac fragment located at the same site but in the opposite orientation was at a lower level and was not repressed by merR+.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2993235

Citation

Foster, T J., and N L. Brown. "Identification of the merR Gene of R100 By Using Mer-lac Gene and Operon Fusions." Journal of Bacteriology, vol. 163, no. 3, 1985, pp. 1153-7.
Foster TJ, Brown NL. Identification of the merR gene of R100 by using mer-lac gene and operon fusions. J Bacteriol. 1985;163(3):1153-7.
Foster, T. J., & Brown, N. L. (1985). Identification of the merR gene of R100 by using mer-lac gene and operon fusions. Journal of Bacteriology, 163(3), pp. 1153-7.
Foster TJ, Brown NL. Identification of the merR Gene of R100 By Using Mer-lac Gene and Operon Fusions. J Bacteriol. 1985;163(3):1153-7. PubMed PMID: 2993235.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of the merR gene of R100 by using mer-lac gene and operon fusions. AU - Foster,T J, AU - Brown,N L, PY - 1985/9/1/pubmed PY - 1985/9/1/medline PY - 1985/9/1/entrez SP - 1153 EP - 7 JF - Journal of bacteriology JO - J. Bacteriol. VL - 163 IS - 3 N2 - Transcriptional (operon) and translational (gene) fusions between the R100 merR gene and lacZ were constructed in vitro in a pBR322 plasmid carrying the mer genes derived from plasmid R100. The translational fusions were oriented in the opposite direction to and divergently from the merTCAD genes. This shows that the reading frame previously thought to be merR was incorrect. Expression of the gene fusion was repressed in trans by a compatible plasmid carrying the R100 merR+ gene, as was a similarly oriented transcriptional fusion. In contrast, expression of beta-galactosidase by the lac fragment located at the same site but in the opposite orientation was at a lower level and was not repressed by merR+. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/2993235/Identification_of_the_merR_gene_of_R100_by_using_mer_lac_gene_and_operon_fusions_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=2993235 DB - PRIME DP - Unbound Medicine ER -