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[Effect of Huangqin Tang on the function of regulatory TLR4/MyD88 signal pathway in rats with ulcerative colitis].
Yao Xue Xue Bao. 2016 10; 51(10):1558-63.YX

Abstract

This study was designed to investigate the effect of Huangqin Tang (HQT) on TLR4/Myd88 pathway and the downstream cytokines in rats with ulcerative colitis (UC) to explore its underlying mechanisms of action. The model of UC rats with cell immunoreactivity was made using a compound method (trinitrobenzene sulfonic acid plus ethanol). Rats were randomly divided into the control group, the model group, the salazosulfapyridine (SASP) group, high, medium and low dose(20, 10, 5 g·kg-1) of HQT groups. After a three-day treatment, production of NO in serum was detected by Griess assay, the levels of interleukin (IL)-4, IL-10, IL-17 and prostaglandin E2(PGE2) in serum were detected by ELISA. After a five-day treatment, the positive protein expressions of COX-2 and iNOS in the colon tissue were determined by ICH method, the protein expressions of TLR4 and MyD88 in colon tissue were determined by Western blot. Compared with the control group, the levels of NO, IL-17, PGE2, the protein expressions of TLR4, MyD88 and the protein positive expressions of COX-2, iNOS were apparently higher in the model group. Compared with model group, the above indexes were significantly improved in the SASP and high-dose HQT groups (P < 0.05). These results show that HQT has a definite effect on UC in rats. Its mechanisms of action may be achieved by inhibiting the activity of TLR4/MyD88/NF-κB signal pathway and down-regulation of NO, IL-17 and PGE2 production.

Authors

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Pub Type(s)

Journal Article

Language

chi

PubMed ID

29932600

Citation

Wang, Dun-fang, et al. "[Effect of Huangqin Tang On the Function of Regulatory TLR4/MyD88 Signal Pathway in Rats With Ulcerative Colitis]." Yao Xue Xue Bao = Acta Pharmaceutica Sinica, vol. 51, no. 10, 2016, pp. 1558-63.
Wang DF, Wang YL, Wang YW, et al. [Effect of Huangqin Tang on the function of regulatory TLR4/MyD88 signal pathway in rats with ulcerative colitis]. Yao Xue Xue Bao. 2016;51(10):1558-63.
Wang, D. F., Wang, Y. L., Wang, Y. W., Guo, S. S., Zhuang, S. X., Xu, H. Y., Li, T., & Yang, W. P. (2016). [Effect of Huangqin Tang on the function of regulatory TLR4/MyD88 signal pathway in rats with ulcerative colitis]. Yao Xue Xue Bao = Acta Pharmaceutica Sinica, 51(10), 1558-63.
Wang DF, et al. [Effect of Huangqin Tang On the Function of Regulatory TLR4/MyD88 Signal Pathway in Rats With Ulcerative Colitis]. Yao Xue Xue Bao. 2016;51(10):1558-63. PubMed PMID: 29932600.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of Huangqin Tang on the function of regulatory TLR4/MyD88 signal pathway in rats with ulcerative colitis]. AU - Wang,Dun-fang, AU - Wang,Yan-li, AU - Wang,Yi-wei, AU - Guo,Shan-shan, AU - Zhuang,Shuai-xing, AU - Xu,Hang-yu, AU - Li,Tao, AU - Yang,Wei-peng, PY - 2018/6/23/entrez PY - 2016/10/1/pubmed PY - 2018/8/22/medline SP - 1558 EP - 63 JF - Yao xue xue bao = Acta pharmaceutica Sinica JO - Yao Xue Xue Bao VL - 51 IS - 10 N2 - This study was designed to investigate the effect of Huangqin Tang (HQT) on TLR4/Myd88 pathway and the downstream cytokines in rats with ulcerative colitis (UC) to explore its underlying mechanisms of action. The model of UC rats with cell immunoreactivity was made using a compound method (trinitrobenzene sulfonic acid plus ethanol). Rats were randomly divided into the control group, the model group, the salazosulfapyridine (SASP) group, high, medium and low dose(20, 10, 5 g·kg-1) of HQT groups. After a three-day treatment, production of NO in serum was detected by Griess assay, the levels of interleukin (IL)-4, IL-10, IL-17 and prostaglandin E2(PGE2) in serum were detected by ELISA. After a five-day treatment, the positive protein expressions of COX-2 and iNOS in the colon tissue were determined by ICH method, the protein expressions of TLR4 and MyD88 in colon tissue were determined by Western blot. Compared with the control group, the levels of NO, IL-17, PGE2, the protein expressions of TLR4, MyD88 and the protein positive expressions of COX-2, iNOS were apparently higher in the model group. Compared with model group, the above indexes were significantly improved in the SASP and high-dose HQT groups (P < 0.05). These results show that HQT has a definite effect on UC in rats. Its mechanisms of action may be achieved by inhibiting the activity of TLR4/MyD88/NF-κB signal pathway and down-regulation of NO, IL-17 and PGE2 production. SN - 0513-4870 UR - https://www.unboundmedicine.com/medline/citation/29932600/[Effect_of_Huangqin_Tang_on_the_function_of_regulatory_TLR4/MyD88_signal_pathway_in_rats_with_ulcerative_colitis]_ L2 - https://medlineplus.gov/ulcerativecolitis.html DB - PRIME DP - Unbound Medicine ER -