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Evaluation of acetamiprid-induced genotoxic and oxidative responses in Eisenia fetida.
Ecotoxicol Environ Saf. 2018 10; 161:610-615.EE

Abstract

As a novel neonicotinoids insecticide, acetamiprid has been widely used worldwide. In this study, a laboratory test was conducted to expose earthworms (Eisenia fetida) to artificial soil spiked with various concentrations of acetamiprid (0, 0.05, 0.10, 0.25 and 0.50 mg/kg of soil) respectively after 7, 14, 21 and 28 d. Reactive oxygen species (ROS) generation, antioxidant enzymes activity including superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferases (GST), malondialdehyde (MDA) content, and DNA damage were determined in earthworms. The ROS level increased in varying degrees at most exposure concentrations. The SOD activity was not significantly affected. The CAT activity was increased in the beginning, then gradually suppressed and resumed to the control level at the end, with the maximum change (171%) occurred at 14 d for 0.05 mg/kg. The GST activity was induced at 7 d, and then inhibited, with the maximum change (67.6%) occurred at 14 d for 0.50 mg/kg. The MDA content had a tendency that increasing at the first and decreasing at the end. The olive tail moment (OTM) in comet assay reflected a dose-dependent relationship, and DNA damage initially increased and then decreased over time. The results suggest that the sub-chronic exposure of acetamiprid can cause oxidative stress and DNA damage of earthworm and change the activity of the anti-oxidant enzyme. In addition, ROS content and DNA damage can be good indicators for assessing environmental risks of acetamiprid in earthworms.

Authors+Show Affiliations

National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, Taian 271018, China.College of Plant Protection, Shandong Agricultural University, Taian 271018, China.National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, Taian 271018, China. Electronic address: wjh@sdau.edu.cn.National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, Taian 271018, China.National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, Taian 271018, China.National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, Taian 271018, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29933130

Citation

Li, Bing, et al. "Evaluation of Acetamiprid-induced Genotoxic and Oxidative Responses in Eisenia Fetida." Ecotoxicology and Environmental Safety, vol. 161, 2018, pp. 610-615.
Li B, Xia X, Wang J, et al. Evaluation of acetamiprid-induced genotoxic and oxidative responses in Eisenia fetida. Ecotoxicol Environ Saf. 2018;161:610-615.
Li, B., Xia, X., Wang, J., Zhu, L., Wang, J., & Wang, G. (2018). Evaluation of acetamiprid-induced genotoxic and oxidative responses in Eisenia fetida. Ecotoxicology and Environmental Safety, 161, 610-615. https://doi.org/10.1016/j.ecoenv.2018.06.022
Li B, et al. Evaluation of Acetamiprid-induced Genotoxic and Oxidative Responses in Eisenia Fetida. Ecotoxicol Environ Saf. 2018;161:610-615. PubMed PMID: 29933130.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of acetamiprid-induced genotoxic and oxidative responses in Eisenia fetida. AU - Li,Bing, AU - Xia,Xiaoming, AU - Wang,Jinhua, AU - Zhu,Lusheng, AU - Wang,Jun, AU - Wang,Guangchi, Y1 - 2018/06/19/ PY - 2018/04/04/received PY - 2018/06/04/revised PY - 2018/06/09/accepted PY - 2018/6/23/pubmed PY - 2019/5/21/medline PY - 2018/6/23/entrez KW - Acetamiprid KW - DNA damage KW - Earthworm KW - Geno-toxicity KW - Oxidative stress SP - 610 EP - 615 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 161 N2 - As a novel neonicotinoids insecticide, acetamiprid has been widely used worldwide. In this study, a laboratory test was conducted to expose earthworms (Eisenia fetida) to artificial soil spiked with various concentrations of acetamiprid (0, 0.05, 0.10, 0.25 and 0.50 mg/kg of soil) respectively after 7, 14, 21 and 28 d. Reactive oxygen species (ROS) generation, antioxidant enzymes activity including superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferases (GST), malondialdehyde (MDA) content, and DNA damage were determined in earthworms. The ROS level increased in varying degrees at most exposure concentrations. The SOD activity was not significantly affected. The CAT activity was increased in the beginning, then gradually suppressed and resumed to the control level at the end, with the maximum change (171%) occurred at 14 d for 0.05 mg/kg. The GST activity was induced at 7 d, and then inhibited, with the maximum change (67.6%) occurred at 14 d for 0.50 mg/kg. The MDA content had a tendency that increasing at the first and decreasing at the end. The olive tail moment (OTM) in comet assay reflected a dose-dependent relationship, and DNA damage initially increased and then decreased over time. The results suggest that the sub-chronic exposure of acetamiprid can cause oxidative stress and DNA damage of earthworm and change the activity of the anti-oxidant enzyme. In addition, ROS content and DNA damage can be good indicators for assessing environmental risks of acetamiprid in earthworms. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/29933130/Evaluation_of_acetamiprid_induced_genotoxic_and_oxidative_responses_in_Eisenia_fetida_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(18)30517-7 DB - PRIME DP - Unbound Medicine ER -