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Developmental toxicity and inhibition of the fungicide hymexazol to melanin biosynthesis in zebrafish embryos.
Pestic Biochem Physiol. 2018 May; 147:139-144.PB

Abstract

Hymexazol is an efficacious and widely used fungicide. However, its environmental toxicological assessment has not been well documented. It had no report of its toxicity to fish embryo. Fish embryo acute toxicity tests are highly predictive of aquatic embryotoxicity outcome. In this study, zebrafish (Danio rerio) embryos were exposed to hymexazol at varying concentrations for the study of the developmental toxicity, melanin biosynthesis, biochemical and transcriptional endpoints. The embryotoxicity tests indicated that the 96h LC50 value of hymexazol was 649mg/L with a 95% confidence interval range of 632-667mg/L. Hymexazol at concentrations of 417-738mg/L decreased the heart rate and increased the voluntary swing. Hymexazol inhibited normal development at concentrations above 554mg/L. the 96h EC50 was 411mg/L. Hymexazol in a concentration range of 417-738mg/L induced cardiac edema and yolk sac edema. Exposure of hymexazol at such concentrations to zebrafish embryos for 48h decreased the pigment area density compared with the no hymexazol control. Tyrosinase activity was inhibited by hymexazol relative to the untreated control. The P53 mRNA expression level in embryos upon exposure to 480mg/L or greater of hymexazol was significantly higher than that of the control. The results indicated that hymexazol has quite low acute toxicity and low embryotoxicity to zebrafish.

Authors+Show Affiliations

College of Environment and Plant Protection, Hainan University, Haikou 570228, China. Electronic address: yongmeifan@126.com.College of Environment and Plant Protection, Hainan University, Haikou 570228, China.College of Environment and Plant Protection, Hainan University, Haikou 570228, China.College of Environment and Plant Protection, Hainan University, Haikou 570228, China.College of Environment and Plant Protection, Hainan University, Haikou 570228, China.Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA. Electronic address: qingl@hawaii.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29933984

Citation

Fan, Yongmei, et al. "Developmental Toxicity and Inhibition of the Fungicide Hymexazol to Melanin Biosynthesis in Zebrafish Embryos." Pesticide Biochemistry and Physiology, vol. 147, 2018, pp. 139-144.
Fan Y, Miao W, Lai K, et al. Developmental toxicity and inhibition of the fungicide hymexazol to melanin biosynthesis in zebrafish embryos. Pestic Biochem Physiol. 2018;147:139-144.
Fan, Y., Miao, W., Lai, K., Huang, W., Song, R., & Li, Q. X. (2018). Developmental toxicity and inhibition of the fungicide hymexazol to melanin biosynthesis in zebrafish embryos. Pesticide Biochemistry and Physiology, 147, 139-144. https://doi.org/10.1016/j.pestbp.2017.10.007
Fan Y, et al. Developmental Toxicity and Inhibition of the Fungicide Hymexazol to Melanin Biosynthesis in Zebrafish Embryos. Pestic Biochem Physiol. 2018;147:139-144. PubMed PMID: 29933984.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Developmental toxicity and inhibition of the fungicide hymexazol to melanin biosynthesis in zebrafish embryos. AU - Fan,Yongmei, AU - Miao,Weiguo, AU - Lai,Kehua, AU - Huang,Weikang, AU - Song,RuiXue, AU - Li,Qing X, Y1 - 2017/10/21/ PY - 2017/04/26/received PY - 2017/09/18/revised PY - 2017/10/20/accepted PY - 2018/6/24/entrez PY - 2018/6/24/pubmed PY - 2018/9/6/medline KW - Aquatic toxicity KW - Fungicide KW - Hymexazol KW - Melanin KW - Tyrosinase KW - Zebrafish SP - 139 EP - 144 JF - Pesticide biochemistry and physiology JO - Pestic Biochem Physiol VL - 147 N2 - Hymexazol is an efficacious and widely used fungicide. However, its environmental toxicological assessment has not been well documented. It had no report of its toxicity to fish embryo. Fish embryo acute toxicity tests are highly predictive of aquatic embryotoxicity outcome. In this study, zebrafish (Danio rerio) embryos were exposed to hymexazol at varying concentrations for the study of the developmental toxicity, melanin biosynthesis, biochemical and transcriptional endpoints. The embryotoxicity tests indicated that the 96h LC50 value of hymexazol was 649mg/L with a 95% confidence interval range of 632-667mg/L. Hymexazol at concentrations of 417-738mg/L decreased the heart rate and increased the voluntary swing. Hymexazol inhibited normal development at concentrations above 554mg/L. the 96h EC50 was 411mg/L. Hymexazol in a concentration range of 417-738mg/L induced cardiac edema and yolk sac edema. Exposure of hymexazol at such concentrations to zebrafish embryos for 48h decreased the pigment area density compared with the no hymexazol control. Tyrosinase activity was inhibited by hymexazol relative to the untreated control. The P53 mRNA expression level in embryos upon exposure to 480mg/L or greater of hymexazol was significantly higher than that of the control. The results indicated that hymexazol has quite low acute toxicity and low embryotoxicity to zebrafish. SN - 1095-9939 UR - https://www.unboundmedicine.com/medline/citation/29933984/Developmental_toxicity_and_inhibition_of_the_fungicide_hymexazol_to_melanin_biosynthesis_in_zebrafish_embryos_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-3575(17)30196-7 DB - PRIME DP - Unbound Medicine ER -