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Cofactor Regeneration Using Permeabilized Escherichia coli Expressing NAD(P)+-Dependent Glycerol-3-Phosphate Dehydrogenase.
J Microbiol Biotechnol. 2018 Aug 28; 28(8):1346-1351.JM

Abstract

Oxidoreductases are effective biocatalysts, but their practical use is limited by the need for large quantities of NAD(P)H. In this study, a whole-cell biocatalyst for NAD(P)H cofactor regeneration was developed using the economical substrate glycerol. This cofactor regeneration system employs permeabilized Escherichia coli cells in which the glpD and gldA genes were deleted and the gpsA gene, which encodes NAD(P)+-dependent glycerol-3-phosphate dehydrogenase, was overexpressed. These manipulations were applied to block a side reaction (i.e., the conversion of glycerol to dihydroxyacetone) and to switch the glpD-encoding enzyme reaction to a gpsA-encoding enzyme reaction that generates both NADH and NADPH. We demonstrated the performance of the cofactor regeneration system using a lactate dehydrogenase reaction as a coupling reaction model. The developed biocatalyst involves an economical substrate, bifunctional regeneration of NAD(P)H, and simple reaction conditions as well as a stable environment for enzymes, and is thus applicable to a variety of oxidoreductase reactions requiring NAD(P)H regeneration.

Authors+Show Affiliations

Department of Chemical and Material Engineering, The University of Suwon, Hwaseong 18323, Republic of Korea.Department of Chemical and Material Engineering, The University of Suwon, Hwaseong 18323, Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29943553

Citation

Rho, Ho Sik, and Kyungoh Choi. "Cofactor Regeneration Using Permeabilized Escherichia Coli Expressing NAD(P)+-Dependent Glycerol-3-Phosphate Dehydrogenase." Journal of Microbiology and Biotechnology, vol. 28, no. 8, 2018, pp. 1346-1351.
Rho HS, Choi K. Cofactor Regeneration Using Permeabilized Escherichia coli Expressing NAD(P)+-Dependent Glycerol-3-Phosphate Dehydrogenase. J Microbiol Biotechnol. 2018;28(8):1346-1351.
Rho, H. S., & Choi, K. (2018). Cofactor Regeneration Using Permeabilized Escherichia coli Expressing NAD(P)+-Dependent Glycerol-3-Phosphate Dehydrogenase. Journal of Microbiology and Biotechnology, 28(8), 1346-1351. https://doi.org/10.4014/jmb.1803.03005
Rho HS, Choi K. Cofactor Regeneration Using Permeabilized Escherichia Coli Expressing NAD(P)+-Dependent Glycerol-3-Phosphate Dehydrogenase. J Microbiol Biotechnol. 2018 Aug 28;28(8):1346-1351. PubMed PMID: 29943553.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cofactor Regeneration Using Permeabilized Escherichia coli Expressing NAD(P)+-Dependent Glycerol-3-Phosphate Dehydrogenase. AU - Rho,Ho Sik, AU - Choi,Kyungoh, PY - 2018/6/27/pubmed PY - 2018/11/27/medline PY - 2018/6/27/entrez KW - Cofactor regeneration KW - NAD(P)H KW - glycerol-3-phosphate dehydrogenase KW - permeabilized whole cell biocatalyst SP - 1346 EP - 1351 JF - Journal of microbiology and biotechnology JO - J. Microbiol. Biotechnol. VL - 28 IS - 8 N2 - Oxidoreductases are effective biocatalysts, but their practical use is limited by the need for large quantities of NAD(P)H. In this study, a whole-cell biocatalyst for NAD(P)H cofactor regeneration was developed using the economical substrate glycerol. This cofactor regeneration system employs permeabilized Escherichia coli cells in which the glpD and gldA genes were deleted and the gpsA gene, which encodes NAD(P)+-dependent glycerol-3-phosphate dehydrogenase, was overexpressed. These manipulations were applied to block a side reaction (i.e., the conversion of glycerol to dihydroxyacetone) and to switch the glpD-encoding enzyme reaction to a gpsA-encoding enzyme reaction that generates both NADH and NADPH. We demonstrated the performance of the cofactor regeneration system using a lactate dehydrogenase reaction as a coupling reaction model. The developed biocatalyst involves an economical substrate, bifunctional regeneration of NAD(P)H, and simple reaction conditions as well as a stable environment for enzymes, and is thus applicable to a variety of oxidoreductase reactions requiring NAD(P)H regeneration. SN - 1738-8872 UR - https://www.unboundmedicine.com/medline/citation/29943553/Cofactor_Regeneration_Using_Permeabilized_Escherichia_coli_Expressing_NAD_P_+_Dependent_Glycerol_3_Phosphate_Dehydrogenase_ L2 - http://www.jmb.or.kr/journal/view.html?doi=10.4014/jmb.1803.03005 DB - PRIME DP - Unbound Medicine ER -