Tags

Type your tag names separated by a space and hit enter

Role of the MAPK/cJun NH2-terminal kinase signaling pathway in starvation-induced autophagy.
Autophagy. 2018; 14(9):1586-1595.A

Abstract

Autophagy is required for cellular homeostasis and can determine cell viability in response to stress. It is established that MTOR is a master regulator of starvation-induced macroautophagy/autophagy, but recent studies have also implicated an essential role for the MAPK8/cJun NH2-terminal kinase 1 signal transduction pathway. We found that MAPK8/JNK1 and MAPK9/JNK2 were not required for autophagy caused by starvation or MTOR inhibition in murine fibroblasts and epithelial cells. These data demonstrate that MAPK8/9 has no required role in starvation-induced autophagy. We conclude that the role of MAPK8/9 in autophagy may be context-dependent and more complex than previously considered.

ABBREVIATIONS

AKT: thymoma viral proto-oncogene;ALB: albumin; ATG4: autophagy related 4; BCL2: B cell leukemia/lymphoma 2; BECN1: beclin 1, autophagy related; BNIP3: BCL2/adenovirus E1B interacting protein 3; CQ: chloroquine diphosphate; DMEM: Dulbecco's modified Eagle's medium; EDTA: ethylenediaminetetraacetic acid; EBSS: Earle's balanced salt solution; FBS: fetal bovine serum; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GFP: green fluorescent protein; HRAS: Harvey rat sarcoma virus oncogene; IgG: Immunoglobulin G; MAPK3/ERK1: mitogen-activated protein kinase 3; MAPK8/JNK1: mitogen-activated protein kinase 8; MAPK9/JNK2: mitogen-activated protein kinase 9; MAPK10/JNK3: mitogen-activated protein kinase 10; MAP1LC3B/LC3B: microtubule-associated protein 1 light chain 3 beta; MEFs: mouse embryonic fibroblasts; MTOR: mechanistic target of rapamycin kinase; RPS6KB1/p70: ribosomal protein S6 kinase, polypeptide 1; PPARA: peroxisome proliferator activated receptor alpha; SEM: standard error of the mean; SQSTM1/p62: sequestosome 1; TORC1: target of rapamycin complex 1; TORC2: target of rapamycin complex 2; TRP53: transforming related protein 53; TUBA: tubulin alpha; UV: ultraviolet; WT: wild-type.

Links

PMC Free PDF

Authors+Show Affiliations

Barutcu SA
a Program in Molecular Medicine , University of Massachusetts Medical School , Worcester , MA , USA.
Girnius N
a Program in Molecular Medicine , University of Massachusetts Medical School , Worcester , MA , USA.
Vernia S
a Program in Molecular Medicine , University of Massachusetts Medical School , Worcester , MA , USA.
Davis RJ
a Program in Molecular Medicine , University of Massachusetts Medical School , Worcester , MA , USA. b Howard Hughes Medical Institute , Worcester , MA , USA.

MeSH

Amino AcidsAnimalsAutophagyCell HypoxiaCells, CulturedEnzyme ActivationEpithelial CellsHepatocytesMAP Kinase Signaling SystemMiceMitogen-Activated Protein Kinase 8Mitogen-Activated Protein Kinase 9NaphthyridinesTOR Serine-Threonine Kinasesras Proteins

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29950132

Citation

Barutcu, Seda Avcioglu, et al. "Role of the MAPK/cJun NH2-terminal Kinase Signaling Pathway in Starvation-induced Autophagy." Autophagy, vol. 14, no. 9, 2018, pp. 1586-1595.
Barutcu SA, Girnius N, Vernia S, et al. Role of the MAPK/cJun NH2-terminal kinase signaling pathway in starvation-induced autophagy. Autophagy. 2018;14(9):1586-1595.
Barutcu, S. A., Girnius, N., Vernia, S., & Davis, R. J. (2018). Role of the MAPK/cJun NH2-terminal kinase signaling pathway in starvation-induced autophagy. Autophagy, 14(9), 1586-1595. https://doi.org/10.1080/15548627.2018.1466013
Barutcu SA, et al. Role of the MAPK/cJun NH2-terminal Kinase Signaling Pathway in Starvation-induced Autophagy. Autophagy. 2018;14(9):1586-1595. PubMed PMID: 29950132.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of the MAPK/cJun NH2-terminal kinase signaling pathway in starvation-induced autophagy. AU - Barutcu,Seda Avcioglu, AU - Girnius,Nomeda, AU - Vernia,Santiago, AU - Davis,Roger J, Y1 - 2018/08/17/ PY - 2018/6/29/pubmed PY - 2019/10/16/medline PY - 2018/6/29/entrez KW - Epithelial cell KW - MAPK8 KW - MAPK9 KW - MTOR KW - fibroblast KW - hepatocyte SP - 1586 EP - 1595 JF - Autophagy JO - Autophagy VL - 14 IS - 9 N2 - : Autophagy is required for cellular homeostasis and can determine cell viability in response to stress. It is established that MTOR is a master regulator of starvation-induced macroautophagy/autophagy, but recent studies have also implicated an essential role for the MAPK8/cJun NH2-terminal kinase 1 signal transduction pathway. We found that MAPK8/JNK1 and MAPK9/JNK2 were not required for autophagy caused by starvation or MTOR inhibition in murine fibroblasts and epithelial cells. These data demonstrate that MAPK8/9 has no required role in starvation-induced autophagy. We conclude that the role of MAPK8/9 in autophagy may be context-dependent and more complex than previously considered. ABBREVIATIONS: AKT: thymoma viral proto-oncogene;ALB: albumin; ATG4: autophagy related 4; BCL2: B cell leukemia/lymphoma 2; BECN1: beclin 1, autophagy related; BNIP3: BCL2/adenovirus E1B interacting protein 3; CQ: chloroquine diphosphate; DMEM: Dulbecco's modified Eagle's medium; EDTA: ethylenediaminetetraacetic acid; EBSS: Earle's balanced salt solution; FBS: fetal bovine serum; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GFP: green fluorescent protein; HRAS: Harvey rat sarcoma virus oncogene; IgG: Immunoglobulin G; MAPK3/ERK1: mitogen-activated protein kinase 3; MAPK8/JNK1: mitogen-activated protein kinase 8; MAPK9/JNK2: mitogen-activated protein kinase 9; MAPK10/JNK3: mitogen-activated protein kinase 10; MAP1LC3B/LC3B: microtubule-associated protein 1 light chain 3 beta; MEFs: mouse embryonic fibroblasts; MTOR: mechanistic target of rapamycin kinase; RPS6KB1/p70: ribosomal protein S6 kinase, polypeptide 1; PPARA: peroxisome proliferator activated receptor alpha; SEM: standard error of the mean; SQSTM1/p62: sequestosome 1; TORC1: target of rapamycin complex 1; TORC2: target of rapamycin complex 2; TRP53: transforming related protein 53; TUBA: tubulin alpha; UV: ultraviolet; WT: wild-type. SN - 1554-8635 UR - https://www.unboundmedicine.com/medline/citation/29950132/Role_of_the_MAPK/cJun_NH2_terminal_kinase_signaling_pathway_in_starvation_induced_autophagy_ DB - PRIME DP - Unbound Medicine ER -