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Green synthesis of gold and silver nanoparticles from Cannabis sativa (industrial hemp) and their capacity for biofilm inhibition.
Int J Nanomedicine. 2018; 13:3571-3591.IJ

Abstract

BACKGROUND

Cannabis sativa (hemp) is a source of various biologically active compounds, for instance, cannabinoids, terpenes and phenolic compounds, which exhibit antibacterial, antifungal, anti-inflammatory and anticancer properties. With the purpose of expanding the auxiliary application of C. sativa in the field of bio-nanotechnology, we explored the plant for green and efficient synthesis of gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs).

METHODS AND RESULTS

The nanoparticles were synthesized by utilizing an aqueous extract of C. sativa stem separated into two different fractions (cortex and core [xylem part]) without any additional reducing, stabilizing and capping agents. In the synthesis of AuNPs using the cortex enriched in bast fibers, fiber-AuNPs (F-AuNPs) were achieved. When using the core part of the stem, which is enriched with phenolic compounds such as alkaloids and cannabinoids, core-AuNPs (C-AuNPs) and core-AgNPs (C-AgNPs) were formed. Synthesized nanoparticles were character-ized by UV-visible analysis, transmission electron microscopy, atomic force microscopy, dynamic light scattering, Fourier transform infrared, and matrix-assisted laser desorption/ionization time-of-flight. In addition, the stable nature of nanoparticles has been shown by thermogravimetric analysis and inductively coupled plasma mass spectrometry (ICP-MS). Finally, the AgNPs were explored for the inhibition of Pseudomonas aeruginosa and Escherichia coli biofilms.

CONCLUSION

The synthesized nanoparticles were crystalline with an average diameter between 12 and 18 nm for F-AuNPs and C-AuNPs and in the range of 20-40 nm for C-AgNPs. ICP-MS analysis revealed concentrations of synthesized nanoparticles as 0.7, 4.5 and 3.6 mg/mL for F-AuNPs, C-AuNPs and C-AgNPs, respectively. Fourier transform infrared spectroscopy revealed the presence of flavonoids, cannabinoids, terpenes and phenols on the nanoparticle surface, which could be responsible for reducing the salts to nanoparticles and further stabilizing them. In addition, the stable nature of synthesized nanoparticles has been shown by thermogravimetric analysis and ICP-MS. Finally, the AgNPs were explored for the inhibition of P. aeruginosa and E. coli biofilms. The nanoparticles exhibited minimum inhibitory concentration values of 6.25 and 5 µg/mL and minimum bactericidal concentration values of 12.5 and 25 µg/mL against P. aeruginosa and E. coli, respectively.

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Authors+Show Affiliations

Singh P
The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark.
Pandit S
Systems and Synthetic Biology Division, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
Garnæs J
Danish Institute of Fundamental Metrology, Lyngby, Denmark.
Tunjic S
Systems and Synthetic Biology Division, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
Mokkapati VR
Systems and Synthetic Biology Division, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
Sultan A
The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark.
Thygesen A
Center for Bioprocess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark, Lyngby, Denmark.
Mackevica A
Department of Environmental Engineering, Technical University of Denmark, Lyngby, Denmark.
Mateiu RV
Department of Chemical and Biochemical Engineering, Technical University of Denmark, Lyngby, Denmark.
Daugaard AE
Danish Polymer Centre, Department of Chemical and Biochemical Engineering, Technical University of Denmark, Lyngby, Denmark.
Baun A
Department of Environmental Engineering, Technical University of Denmark, Lyngby, Denmark.
Mijakovic I
The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark. Systems and Synthetic Biology Division, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.

MeSH

Anti-Bacterial AgentsBiofilmsCannabisDynamic Light ScatteringEscherichia coliGoldGreen Chemistry TechnologyHumansIonsKineticsMetal NanoparticlesMicrobial Sensitivity TestsParticle SizePlant ExtractsPseudomonas aeruginosaSilverSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSpectrophotometry, UltravioletSpectroscopy, Fourier Transform Infrared

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29950836

Citation

Singh, Priyanka, et al. "Green Synthesis of Gold and Silver Nanoparticles From Cannabis Sativa (industrial Hemp) and Their Capacity for Biofilm Inhibition." International Journal of Nanomedicine, vol. 13, 2018, pp. 3571-3591.
Singh P, Pandit S, Garnæs J, et al. Green synthesis of gold and silver nanoparticles from Cannabis sativa (industrial hemp) and their capacity for biofilm inhibition. Int J Nanomedicine. 2018;13:3571-3591.
Singh, P., Pandit, S., Garnæs, J., Tunjic, S., Mokkapati, V. R., Sultan, A., Thygesen, A., Mackevica, A., Mateiu, R. V., Daugaard, A. E., Baun, A., & Mijakovic, I. (2018). Green synthesis of gold and silver nanoparticles from Cannabis sativa (industrial hemp) and their capacity for biofilm inhibition. International Journal of Nanomedicine, 13, 3571-3591. https://doi.org/10.2147/IJN.S157958
Singh P, et al. Green Synthesis of Gold and Silver Nanoparticles From Cannabis Sativa (industrial Hemp) and Their Capacity for Biofilm Inhibition. Int J Nanomedicine. 2018;13:3571-3591. PubMed PMID: 29950836.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Green synthesis of gold and silver nanoparticles from Cannabis sativa (industrial hemp) and their capacity for biofilm inhibition. AU - Singh,Priyanka, AU - Pandit,Santosh, AU - Garnæs,Jørgen, AU - Tunjic,Sanja, AU - Mokkapati,Venkata Rss, AU - Sultan,Abida, AU - Thygesen,Anders, AU - Mackevica,Aiga, AU - Mateiu,Ramona Valentina, AU - Daugaard,Anders Egede, AU - Baun,Anders, AU - Mijakovic,Ivan, Y1 - 2018/06/21/ PY - 2018/6/29/entrez PY - 2018/6/29/pubmed PY - 2018/7/28/medline KW - Cannabis sativa KW - ICP-MS KW - biofilm KW - biomedical application KW - gold nanoparticles KW - hemp KW - medicinal plant KW - nanoparticle concentration KW - nanotechnology KW - pathogenic strains KW - silver nanoparticles SP - 3571 EP - 3591 JF - International journal of nanomedicine JO - Int J Nanomedicine VL - 13 N2 - BACKGROUND: Cannabis sativa (hemp) is a source of various biologically active compounds, for instance, cannabinoids, terpenes and phenolic compounds, which exhibit antibacterial, antifungal, anti-inflammatory and anticancer properties. With the purpose of expanding the auxiliary application of C. sativa in the field of bio-nanotechnology, we explored the plant for green and efficient synthesis of gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs). METHODS AND RESULTS: The nanoparticles were synthesized by utilizing an aqueous extract of C. sativa stem separated into two different fractions (cortex and core [xylem part]) without any additional reducing, stabilizing and capping agents. In the synthesis of AuNPs using the cortex enriched in bast fibers, fiber-AuNPs (F-AuNPs) were achieved. When using the core part of the stem, which is enriched with phenolic compounds such as alkaloids and cannabinoids, core-AuNPs (C-AuNPs) and core-AgNPs (C-AgNPs) were formed. Synthesized nanoparticles were character-ized by UV-visible analysis, transmission electron microscopy, atomic force microscopy, dynamic light scattering, Fourier transform infrared, and matrix-assisted laser desorption/ionization time-of-flight. In addition, the stable nature of nanoparticles has been shown by thermogravimetric analysis and inductively coupled plasma mass spectrometry (ICP-MS). Finally, the AgNPs were explored for the inhibition of Pseudomonas aeruginosa and Escherichia coli biofilms. CONCLUSION: The synthesized nanoparticles were crystalline with an average diameter between 12 and 18 nm for F-AuNPs and C-AuNPs and in the range of 20-40 nm for C-AgNPs. ICP-MS analysis revealed concentrations of synthesized nanoparticles as 0.7, 4.5 and 3.6 mg/mL for F-AuNPs, C-AuNPs and C-AgNPs, respectively. Fourier transform infrared spectroscopy revealed the presence of flavonoids, cannabinoids, terpenes and phenols on the nanoparticle surface, which could be responsible for reducing the salts to nanoparticles and further stabilizing them. In addition, the stable nature of synthesized nanoparticles has been shown by thermogravimetric analysis and ICP-MS. Finally, the AgNPs were explored for the inhibition of P. aeruginosa and E. coli biofilms. The nanoparticles exhibited minimum inhibitory concentration values of 6.25 and 5 µg/mL and minimum bactericidal concentration values of 12.5 and 25 µg/mL against P. aeruginosa and E. coli, respectively. SN - 1178-2013 UR - https://www.unboundmedicine.com/medline/citation/29950836/Green_synthesis_of_gold_and_silver_nanoparticles_from_Cannabis_sativa__industrial_hemp__and_their_capacity_for_biofilm_inhibition_ DB - PRIME DP - Unbound Medicine ER -