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Determination of Antioxidant Potential of Selected Wild Edible Mushrooms from India in a Saccharomyces cerevisiae Model System.
Int J Med Mushrooms. 2018; 20(6):569-580.IJ

Abstract

Wild edible mushrooms are one of the most fascinating nutraceuticals because of their pleasant texture, peculiar aroma, and tremendous therapeutic potential; they have been used since ancient times. In this study we evaluated the antioxidant potential of 4 wild edible mushrooms-Cantharellus tropicalis, C. cibarius, Lentinus edodes, and Russula delica-collected from Mizoram, India. We performed in vitro and in vivo studies using a Saccharomyces cerevisiae stress response mechanism as the model system. Among the 4 mushrooms, C. cibarius exhibited the most significant antioxidant activity both in vitro and in vivo, followed by L. edodes, R. delica, and C. tropicalis. Gas chromatography-mass spectrometry analysis of methanolic extracts of the mushrooms revealed the presence of substantial amounts of fatty acids, fatty acid esters, and other bioactive constituents. The in vitro antioxidant activity was corroborated by in vivo studies using an S. cerevisiae oxidative stress response mechanism. In spot assays, the C. cibarius methanolic extract showed the highest scavenging potential in wild and mutant (sodlΔ and tsalΔ) strains of S. cerevisiae. These results were confirmed further by determining the level of reactive oxygen species through the use of fluorescent microscopy and intensity studies. The results suggested the efficacy of wild edible mushrooms as prominent therapeutic agents and that they have tremendous nutraceutical properties.

Authors+Show Affiliations

Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry, India.Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry, India.Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Puducherry, India; DBT-Interdisciplinary Program in Life Sciences, School of Life Sciences, Pondicherry University, Puducherry, India.Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Puducherry, India; DBT-Interdisciplinary Program in Life Sciences, School of Life Sciences, Pondicherry University, Puducherry, India.Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29953353

Citation

Pattnaik, Subhaswaraj, et al. "Determination of Antioxidant Potential of Selected Wild Edible Mushrooms From India in a Saccharomyces Cerevisiae Model System." International Journal of Medicinal Mushrooms, vol. 20, no. 6, 2018, pp. 569-580.
Pattnaik S, Hnamte S, Sudharshan SJ, et al. Determination of Antioxidant Potential of Selected Wild Edible Mushrooms from India in a Saccharomyces cerevisiae Model System. Int J Med Mushrooms. 2018;20(6):569-580.
Pattnaik, S., Hnamte, S., Sudharshan, S. J., Dyavaiah, M., & Busi, S. (2018). Determination of Antioxidant Potential of Selected Wild Edible Mushrooms from India in a Saccharomyces cerevisiae Model System. International Journal of Medicinal Mushrooms, 20(6), 569-580. https://doi.org/10.1615/IntJMedMushrooms.2018026531
Pattnaik S, et al. Determination of Antioxidant Potential of Selected Wild Edible Mushrooms From India in a Saccharomyces Cerevisiae Model System. Int J Med Mushrooms. 2018;20(6):569-580. PubMed PMID: 29953353.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of Antioxidant Potential of Selected Wild Edible Mushrooms from India in a Saccharomyces cerevisiae Model System. AU - Pattnaik,Subhaswaraj, AU - Hnamte,Sairengpuii, AU - Sudharshan,S J, AU - Dyavaiah,Madhu, AU - Busi,Siddhardha, PY - 2018/6/29/entrez PY - 2018/6/29/pubmed PY - 2018/10/17/medline SP - 569 EP - 580 JF - International journal of medicinal mushrooms JO - Int J Med Mushrooms VL - 20 IS - 6 N2 - Wild edible mushrooms are one of the most fascinating nutraceuticals because of their pleasant texture, peculiar aroma, and tremendous therapeutic potential; they have been used since ancient times. In this study we evaluated the antioxidant potential of 4 wild edible mushrooms-Cantharellus tropicalis, C. cibarius, Lentinus edodes, and Russula delica-collected from Mizoram, India. We performed in vitro and in vivo studies using a Saccharomyces cerevisiae stress response mechanism as the model system. Among the 4 mushrooms, C. cibarius exhibited the most significant antioxidant activity both in vitro and in vivo, followed by L. edodes, R. delica, and C. tropicalis. Gas chromatography-mass spectrometry analysis of methanolic extracts of the mushrooms revealed the presence of substantial amounts of fatty acids, fatty acid esters, and other bioactive constituents. The in vitro antioxidant activity was corroborated by in vivo studies using an S. cerevisiae oxidative stress response mechanism. In spot assays, the C. cibarius methanolic extract showed the highest scavenging potential in wild and mutant (sodlΔ and tsalΔ) strains of S. cerevisiae. These results were confirmed further by determining the level of reactive oxygen species through the use of fluorescent microscopy and intensity studies. The results suggested the efficacy of wild edible mushrooms as prominent therapeutic agents and that they have tremendous nutraceutical properties. SN - 1940-4344 UR - https://www.unboundmedicine.com/medline/citation/29953353/Determination_of_Antioxidant_Potential_of_Selected_Wild_Edible_Mushrooms_from_India_in_a_Saccharomyces_cerevisiae_Model_System_ DB - PRIME DP - Unbound Medicine ER -