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Evidence of zoonotic leprosy in Pará, Brazilian Amazon, and risks associated with human contact or consumption of armadillos.
PLoS Negl Trop Dis. 2018 06; 12(6):e0006532.PN

Abstract

Mycobacterium leprae (M. leprae) is a human pathogen and the causative agent for leprosy, a chronic disease characterized by lesions of the skin and peripheral nerve damage. Zoonotic transmission of M. leprae to humans by nine-banded armadillos (Dasypus novemcinctus) has been shown to occur in the southern United States, mainly in Texas, Louisiana, and Florida. Nine-banded armadillos are also common in South America, and residents living in some areas in Brazil hunt and kill armadillos as a dietary source of protein. This study examines the extent of M. leprae infection in wild armadillos and whether these New World mammals may be a natural reservoir for leprosy transmission in Brazil, similar to the situation in the southern states of the U.S. The presence of the M. leprae-specific repetitive sequence RLEP was detected by PCR amplification in purified DNA extracted from armadillo spleen and liver tissue samples. A positive RLEP signal was confirmed in 62% of the armadillos (10/16), indicating high rates of infection with M. leprae. Immunohistochemistry of sections of infected armadillo spleens revealed mycobacterial DNA and cell wall constituents in situ detected by SYBR Gold and auramine/rhodamine staining techniques, respectively. The M. leprae-specific antigen, phenolic glycolipid I (PGL-I) was detected in spleen sections using a rabbit polyclonal antibody specific for PGL-I. Anti-PGL-I titers were assessed by ELISA in sera from 146 inhabitants of Belterra, a hyperendemic city located in western Pará state in Brazil. A positive anti-PGL-I titer is a known biomarker for M. leprae infection in both humans and armadillos. Individuals who consumed armadillo meat most frequently (more than once per month) showed a significantly higher anti-PGL-I titer than those who did not eat or ate less frequently than once per month. Armadillos infected with M. leprae represent a potential environmental reservoir. Consequently, people who hunt, kill, or process or eat armadillo meat are at a higher risk for infection with M. leprae from these animals.

Authors+Show Affiliations

Laboratório de Dermato-Imunologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Marituba, Pará, Brazil.Universidade Federal do Oeste do Pará, Santarém, Pará, Brazil.Department of Microbiology, Immunology, and Pathology, Mycobacteria Research Laboratories, Colorado State University, Fort Collins, Colorado, United States of America.Department of Microbiology, Immunology, and Pathology, Mycobacteria Research Laboratories, Colorado State University, Fort Collins, Colorado, United States of America.Department of Microbiology, Immunology, and Pathology, Mycobacteria Research Laboratories, Colorado State University, Fort Collins, Colorado, United States of America.Department of Microbiology, Immunology, and Pathology, Mycobacteria Research Laboratories, Colorado State University, Fort Collins, Colorado, United States of America.Department of Microbiology, Immunology, and Pathology, Mycobacteria Research Laboratories, Colorado State University, Fort Collins, Colorado, United States of America.Laboratório de Dermato-Imunologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Marituba, Pará, Brazil. Unidade de Referência Especializada em Dermatologia Sanitária do Estado do Pará - URE Dr. Marcelo Candia, Marituba, Pará, Brazil.Laboratório de Dermato-Imunologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Marituba, Pará, Brazil.Laboratório de Dermato-Imunologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Marituba, Pará, Brazil. Spatial Epidemiology Laboratory, Universidade Federal do Pará, Campus Castanhal, Pará, Brazil.Universidade Federal do Oeste do Pará, Santarém, Pará, Brazil.École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.Dermatology Division of the Department of Internal Medicine, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil.Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.Laboratório de Dermato-Imunologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Marituba, Pará, Brazil. Unidade de Referência Especializada em Dermatologia Sanitária do Estado do Pará - URE Dr. Marcelo Candia, Marituba, Pará, Brazil.Department of Microbiology, Immunology, and Pathology, Mycobacteria Research Laboratories, Colorado State University, Fort Collins, Colorado, United States of America.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29953440

Citation

da Silva, Moises B., et al. "Evidence of Zoonotic Leprosy in Pará, Brazilian Amazon, and Risks Associated With Human Contact or Consumption of Armadillos." PLoS Neglected Tropical Diseases, vol. 12, no. 6, 2018, pp. e0006532.
da Silva MB, Portela JM, Li W, et al. Evidence of zoonotic leprosy in Pará, Brazilian Amazon, and risks associated with human contact or consumption of armadillos. PLoS Negl Trop Dis. 2018;12(6):e0006532.
da Silva, M. B., Portela, J. M., Li, W., Jackson, M., Gonzalez-Juarrero, M., Hidalgo, A. S., Belisle, J. T., Bouth, R. C., Gobbo, A. R., Barreto, J. G., Minervino, A. H. H., Cole, S. T., Avanzi, C., Busso, P., Frade, M. A. C., Geluk, A., Salgado, C. G., & Spencer, J. S. (2018). Evidence of zoonotic leprosy in Pará, Brazilian Amazon, and risks associated with human contact or consumption of armadillos. PLoS Neglected Tropical Diseases, 12(6), e0006532. https://doi.org/10.1371/journal.pntd.0006532
da Silva MB, et al. Evidence of Zoonotic Leprosy in Pará, Brazilian Amazon, and Risks Associated With Human Contact or Consumption of Armadillos. PLoS Negl Trop Dis. 2018;12(6):e0006532. PubMed PMID: 29953440.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence of zoonotic leprosy in Pará, Brazilian Amazon, and risks associated with human contact or consumption of armadillos. AU - da Silva,Moises B, AU - Portela,Juliana M, AU - Li,Wei, AU - Jackson,Mary, AU - Gonzalez-Juarrero,Mercedes, AU - Hidalgo,Andrea Sánchez, AU - Belisle,John T, AU - Bouth,Raquel C, AU - Gobbo,Angélica R, AU - Barreto,Josafá G, AU - Minervino,Antonio H H, AU - Cole,Stewart T, AU - Avanzi,Charlotte, AU - Busso,Philippe, AU - Frade,Marco A C, AU - Geluk,Annemieke, AU - Salgado,Claudio G, AU - Spencer,John S, Y1 - 2018/06/28/ PY - 2017/02/17/received PY - 2018/05/15/accepted PY - 2018/6/29/entrez PY - 2018/6/29/pubmed PY - 2018/8/14/medline SP - e0006532 EP - e0006532 JF - PLoS neglected tropical diseases JO - PLoS Negl Trop Dis VL - 12 IS - 6 N2 - Mycobacterium leprae (M. leprae) is a human pathogen and the causative agent for leprosy, a chronic disease characterized by lesions of the skin and peripheral nerve damage. Zoonotic transmission of M. leprae to humans by nine-banded armadillos (Dasypus novemcinctus) has been shown to occur in the southern United States, mainly in Texas, Louisiana, and Florida. Nine-banded armadillos are also common in South America, and residents living in some areas in Brazil hunt and kill armadillos as a dietary source of protein. This study examines the extent of M. leprae infection in wild armadillos and whether these New World mammals may be a natural reservoir for leprosy transmission in Brazil, similar to the situation in the southern states of the U.S. The presence of the M. leprae-specific repetitive sequence RLEP was detected by PCR amplification in purified DNA extracted from armadillo spleen and liver tissue samples. A positive RLEP signal was confirmed in 62% of the armadillos (10/16), indicating high rates of infection with M. leprae. Immunohistochemistry of sections of infected armadillo spleens revealed mycobacterial DNA and cell wall constituents in situ detected by SYBR Gold and auramine/rhodamine staining techniques, respectively. The M. leprae-specific antigen, phenolic glycolipid I (PGL-I) was detected in spleen sections using a rabbit polyclonal antibody specific for PGL-I. Anti-PGL-I titers were assessed by ELISA in sera from 146 inhabitants of Belterra, a hyperendemic city located in western Pará state in Brazil. A positive anti-PGL-I titer is a known biomarker for M. leprae infection in both humans and armadillos. Individuals who consumed armadillo meat most frequently (more than once per month) showed a significantly higher anti-PGL-I titer than those who did not eat or ate less frequently than once per month. Armadillos infected with M. leprae represent a potential environmental reservoir. Consequently, people who hunt, kill, or process or eat armadillo meat are at a higher risk for infection with M. leprae from these animals. SN - 1935-2735 UR - https://www.unboundmedicine.com/medline/citation/29953440/Evidence_of_zoonotic_leprosy_in_Pará_Brazilian_Amazon_and_risks_associated_with_human_contact_or_consumption_of_armadillos_ DB - PRIME DP - Unbound Medicine ER -