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Chemical Composition, Cytotoxic and Antibacterial Activities of Essential Oils of Cultivated Clones of Juniperus communis and Wild Juniperus Species.
Chem Biodivers. 2018 Sep; 15(9):e1800183.CB

Abstract

Needles of seven cultivated clones (C1 - C7) of Juniperus communis at lower altitude and three wild Juniperus species (J. communis, J. recurva and J. indica) at higher altitudes were investigated comparatively for their essential oils (EOs) yields, chemical composition, cytotoxic and antibacterial activities. The EOs yields varied from 0.26 to 0.56% (v/w) among samples. Sixty-one volatile components were identified by gas chromatography-mass spectrometry (GC/MS) and quantified using gas chromatography GC (FID) representing 82.5 - 95.7% of the total oil. Monoterpene hydrocarbons (49.1 - 82.8%) dominated in all samples (α-pinene, limonene and sabinene as major components). Principal component analysis (PCA) of GC data revealed that wild and cultivated Juniperus species are highly distinct due to variation in chemical composition. J. communis (wild species) displayed cytotoxicity against SiHa (human cervical cancer), A549 (human lung carcinoma) and A431 (human skin carcinoma) cells (66.4 ± 2.2%, 74.4 ± 1.4% and 57.4 ± 4.0%), respectively, at 200 μg/ml. EOs exhibited better antibacterial activity against Gram-positive bacteria than against Gram-negative bacteria with the highest zone of inhibition against Staphylococcus aureus MTCC 96 (19.2 ± 0.7) by clone-7. As per the conclusion of the findings, EOs of clone-2, clone-5 and clone-7 can be suggested to the growers of lower altitude, as there is more possibility of uses of these EOs in food and medicinal preparations.

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Authors+Show Affiliations

Maurya AK
Natural Product Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India. Academy of Scientific and Innovative Research, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India.
Devi R
Natural Product Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India.
Kumar A
Natural Product Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India. Academy of Scientific and Innovative Research, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India.
Koundal R
Natural Product Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India.
Thakur S
Natural Product Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India.
Sharma A
Academy of Scientific and Innovative Research, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India. Pharmacology and Toxicology Lab, Food and Nutraceuticals Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India.
Kumar D
Pharmacology and Toxicology Lab, Food and Nutraceuticals Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India.
Kumar R
Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India.
Padwad YS
Pharmacology and Toxicology Lab, Food and Nutraceuticals Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India.
Chand G
Biodiversity Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India.
Singh B
Natural Product Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India. Academy of Scientific and Innovative Research, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India.
Agnihotri VK
Natural Product Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India. Academy of Scientific and Innovative Research, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India.

MeSH

Anti-Bacterial AgentsAntineoplastic Agents, PhytogenicBacillus subtilisCell ProliferationCloning, MolecularDose-Response Relationship, DrugDrug Screening Assays, AntitumorHumansJuniperusMicrobial Sensitivity TestsMicrococcus luteusMolecular StructureOils, VolatileSeedsSpecies SpecificityStaphylococcus aureusStructure-Activity RelationshipTumor Cells, Cultured

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29956891

Citation

Maurya, Antim K., et al. "Chemical Composition, Cytotoxic and Antibacterial Activities of Essential Oils of Cultivated Clones of Juniperus Communis and Wild Juniperus Species." Chemistry & Biodiversity, vol. 15, no. 9, 2018, pp. e1800183.
Maurya AK, Devi R, Kumar A, et al. Chemical Composition, Cytotoxic and Antibacterial Activities of Essential Oils of Cultivated Clones of Juniperus communis and Wild Juniperus Species. Chem Biodivers. 2018;15(9):e1800183.
Maurya, A. K., Devi, R., Kumar, A., Koundal, R., Thakur, S., Sharma, A., Kumar, D., Kumar, R., Padwad, Y. S., Chand, G., Singh, B., & Agnihotri, V. K. (2018). Chemical Composition, Cytotoxic and Antibacterial Activities of Essential Oils of Cultivated Clones of Juniperus communis and Wild Juniperus Species. Chemistry & Biodiversity, 15(9), e1800183. https://doi.org/10.1002/cbdv.201800183
Maurya AK, et al. Chemical Composition, Cytotoxic and Antibacterial Activities of Essential Oils of Cultivated Clones of Juniperus Communis and Wild Juniperus Species. Chem Biodivers. 2018;15(9):e1800183. PubMed PMID: 29956891.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical Composition, Cytotoxic and Antibacterial Activities of Essential Oils of Cultivated Clones of Juniperus communis and Wild Juniperus Species. AU - Maurya,Antim K, AU - Devi,Renuka, AU - Kumar,Ashish, AU - Koundal,Rajkesh, AU - Thakur,Soni, AU - Sharma,Anamika, AU - Kumar,Dharmesh, AU - Kumar,Rakshak, AU - Padwad,Yogendra S, AU - Chand,Gopi, AU - Singh,Bikram, AU - Agnihotri,Vijai K, Y1 - 2018/08/16/ PY - 2018/04/21/received PY - 2018/06/27/accepted PY - 2018/6/30/pubmed PY - 2018/10/16/medline PY - 2018/6/30/entrez KW - Juniperus species KW - antibacterial activities KW - cytotoxic activities KW - essential oils KW - principal component analysis SP - e1800183 EP - e1800183 JF - Chemistry & biodiversity JO - Chem Biodivers VL - 15 IS - 9 N2 - Needles of seven cultivated clones (C1 - C7) of Juniperus communis at lower altitude and three wild Juniperus species (J. communis, J. recurva and J. indica) at higher altitudes were investigated comparatively for their essential oils (EOs) yields, chemical composition, cytotoxic and antibacterial activities. The EOs yields varied from 0.26 to 0.56% (v/w) among samples. Sixty-one volatile components were identified by gas chromatography-mass spectrometry (GC/MS) and quantified using gas chromatography GC (FID) representing 82.5 - 95.7% of the total oil. Monoterpene hydrocarbons (49.1 - 82.8%) dominated in all samples (α-pinene, limonene and sabinene as major components). Principal component analysis (PCA) of GC data revealed that wild and cultivated Juniperus species are highly distinct due to variation in chemical composition. J. communis (wild species) displayed cytotoxicity against SiHa (human cervical cancer), A549 (human lung carcinoma) and A431 (human skin carcinoma) cells (66.4 ± 2.2%, 74.4 ± 1.4% and 57.4 ± 4.0%), respectively, at 200 μg/ml. EOs exhibited better antibacterial activity against Gram-positive bacteria than against Gram-negative bacteria with the highest zone of inhibition against Staphylococcus aureus MTCC 96 (19.2 ± 0.7) by clone-7. As per the conclusion of the findings, EOs of clone-2, clone-5 and clone-7 can be suggested to the growers of lower altitude, as there is more possibility of uses of these EOs in food and medicinal preparations. SN - 1612-1880 UR - https://www.unboundmedicine.com/medline/citation/29956891/Chemical_Composition_Cytotoxic_and_Antibacterial_Activities_of_Essential_Oils_of_Cultivated_Clones_of_Juniperus_communis_and_Wild_Juniperus_Species_ DB - PRIME DP - Unbound Medicine ER -