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[Molecular organization of plasmid R906 (Inc P-1)].
Mol Biol (Mosk). 1985 Jul-Aug; 19(4):964-73.MB

Abstract

Genetic and restriction (for enzymes EcoRI, BamHI and HindIII) maps of the relatively broad host range plasmid R906 are constructed. There are two non-essential regions on the R906 DNA which can be deleted and cloned. Non-essential regions confer a resistance to different agents and restriction sites are clustered in these regions. Essential and conjugativity genes are located in two other DNA regions approximately at 0-23 and 29-44 kb of the R906 map. These large regions share a high level of homology with Inc-1 group plasmids R751 and RP4 according to Southern-blot hybridization and heteroduplex analyses. A transposon-like structure is found on the R751 DNA among R751/R906 heteroduplex molecules. This transposon of total length 5.1 kb has 1.4 kb inverted repeats at the ends. Bla genes of R906 and RP4 plasmids do not have homologous sequences. Data evidence that IncP-1 group plasmids irrespective to their original bacterial source and range of coded antibiotic resistance have very similar molecular organization. The role of possible factors which are responsible for the broad host range property of the IncP-1 group plasmids is discussed.

Authors

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Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

2995794

Citation

Sakanian, V A., et al. "[Molecular Organization of Plasmid R906 (Inc P-1)]." Molekuliarnaia Biologiia, vol. 19, no. 4, 1985, pp. 964-73.
Sakanian VA, Azarian NG, Krupenko MA. [Molecular organization of plasmid R906 (Inc P-1)]. Mol Biol (Mosk). 1985;19(4):964-73.
Sakanian, V. A., Azarian, N. G., & Krupenko, M. A. (1985). [Molecular organization of plasmid R906 (Inc P-1)]. Molekuliarnaia Biologiia, 19(4), 964-73.
Sakanian VA, Azarian NG, Krupenko MA. [Molecular Organization of Plasmid R906 (Inc P-1)]. Mol Biol (Mosk). 1985 Jul-Aug;19(4):964-73. PubMed PMID: 2995794.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Molecular organization of plasmid R906 (Inc P-1)]. AU - Sakanian,V A, AU - Azarian,N G, AU - Krupenko,M A, PY - 1985/7/1/pubmed PY - 1985/7/1/medline PY - 1985/7/1/entrez SP - 964 EP - 73 JF - Molekuliarnaia biologiia JO - Mol. Biol. (Mosk.) VL - 19 IS - 4 N2 - Genetic and restriction (for enzymes EcoRI, BamHI and HindIII) maps of the relatively broad host range plasmid R906 are constructed. There are two non-essential regions on the R906 DNA which can be deleted and cloned. Non-essential regions confer a resistance to different agents and restriction sites are clustered in these regions. Essential and conjugativity genes are located in two other DNA regions approximately at 0-23 and 29-44 kb of the R906 map. These large regions share a high level of homology with Inc-1 group plasmids R751 and RP4 according to Southern-blot hybridization and heteroduplex analyses. A transposon-like structure is found on the R751 DNA among R751/R906 heteroduplex molecules. This transposon of total length 5.1 kb has 1.4 kb inverted repeats at the ends. Bla genes of R906 and RP4 plasmids do not have homologous sequences. Data evidence that IncP-1 group plasmids irrespective to their original bacterial source and range of coded antibiotic resistance have very similar molecular organization. The role of possible factors which are responsible for the broad host range property of the IncP-1 group plasmids is discussed. SN - 0026-8984 UR - https://www.unboundmedicine.com/medline/citation/2995794/[Molecular_organization_of_plasmid_R906__Inc_P_1_]_ DB - PRIME DP - Unbound Medicine ER -