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Simultaneous Determination and Pharmacokinetics of Peimine and Peiminine in Beagle Dog Plasma by UPLC-MS/MS after the Oral Administration of Fritillariae ussuriensis Maxim and Fritillariae thunbergii Miq Powder.
Molecules 2018; 23(7)M

Abstract

A simple and high sensitive ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the simultaneous determination of peimine and peiminine in beagle dog plasma after the oral administration of Fritillariae ussuriensis Maxim and Fritillariae thunbergii Miq powder. Chromatographic separation was achieved on an ACQUIT UPLC® BEH C18 column (1.7 μm, 2.1 × 100 mm) in a gradient elution way with a mobile phase consisting of acetonitrile and water containing 0.1% formic acid at a flow rate of 0.4 mL/min. The plasma samples were prepared by a liquid⁻liquid extraction (LLE) method with ethyl acetate. The analytes were detected with a triple quadrupole tandem mass spectrometry (MS) in multiple reaction monitoring (MRM) mode and a positive ion electrospray ionization (ESI) of the transitions at m/z 432.4→414.4 for peimine and m/z 430.3→412.3 for peiminine. The method was linear for two analytes over the investigated range with all determined correlation coefficients exceeding 0.9900. The lower limit of quantification (LLOQ) was 0.988 ng/mL for peimine and 0.980 ng/mL for peiminine. The mean extraction recoveries of peimine and peiminine at three quality control samples (QC) levels were ranged from 82.56 to 88.71%, and matrix effects ranged from 92.06 to 101.2%. The intra-day and inter-day precision and accuracy were within the acceptable limits at LLOQ and QC levels. The method was effectively and successfully applied to the pharmacokinetics of peimine and peiminine after oral administration of powder to beagle dogs. The obtained results may be help to guide the clinical application of Fritillaria ussuriensis Maxim and Fritillaria thunbergii Miq.

Authors+Show Affiliations

School of Pharmacy, Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin 150040, Heilongjiang, China. wzbmailbox@hljucm.net.School of Pharmacy, Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin 150040, Heilongjiang, China. 18435166854@163.com.School of Pharmacy, Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin 150040, Heilongjiang, China. 18846925019@163.com.School of Pharmacy, Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin 150040, Heilongjiang, China. 13199472861@163.com.School of Pharmacy, Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin 150040, Heilongjiang, China. wangzhen_yue@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29958456

Citation

Wang, Zhibin, et al. "Simultaneous Determination and Pharmacokinetics of Peimine and Peiminine in Beagle Dog Plasma By UPLC-MS/MS After the Oral Administration of Fritillariae Ussuriensis Maxim and Fritillariae Thunbergii Miq Powder." Molecules (Basel, Switzerland), vol. 23, no. 7, 2018.
Wang Z, Cao F, Chen Y, et al. Simultaneous Determination and Pharmacokinetics of Peimine and Peiminine in Beagle Dog Plasma by UPLC-MS/MS after the Oral Administration of Fritillariae ussuriensis Maxim and Fritillariae thunbergii Miq Powder. Molecules. 2018;23(7).
Wang, Z., Cao, F., Chen, Y., Tang, Z., & Wang, Z. (2018). Simultaneous Determination and Pharmacokinetics of Peimine and Peiminine in Beagle Dog Plasma by UPLC-MS/MS after the Oral Administration of Fritillariae ussuriensis Maxim and Fritillariae thunbergii Miq Powder. Molecules (Basel, Switzerland), 23(7), doi:10.3390/molecules23071573.
Wang Z, et al. Simultaneous Determination and Pharmacokinetics of Peimine and Peiminine in Beagle Dog Plasma By UPLC-MS/MS After the Oral Administration of Fritillariae Ussuriensis Maxim and Fritillariae Thunbergii Miq Powder. Molecules. 2018 06 28;23(7) PubMed PMID: 29958456.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous Determination and Pharmacokinetics of Peimine and Peiminine in Beagle Dog Plasma by UPLC-MS/MS after the Oral Administration of Fritillariae ussuriensis Maxim and Fritillariae thunbergii Miq Powder. AU - Wang,Zhibin, AU - Cao,Feng, AU - Chen,Yajun, AU - Tang,Zhenqiu, AU - Wang,Zhenyue, Y1 - 2018/06/28/ PY - 2018/05/09/received PY - 2018/06/18/revised PY - 2018/06/21/accepted PY - 2018/7/1/entrez PY - 2018/7/1/pubmed PY - 2018/10/17/medline KW - Fritillaria KW - UPLC-MS/MS KW - dog plasma KW - isosteroidal alkaloids KW - pharmacokinetics JF - Molecules (Basel, Switzerland) JO - Molecules VL - 23 IS - 7 N2 - A simple and high sensitive ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the simultaneous determination of peimine and peiminine in beagle dog plasma after the oral administration of Fritillariae ussuriensis Maxim and Fritillariae thunbergii Miq powder. Chromatographic separation was achieved on an ACQUIT UPLC® BEH C18 column (1.7 μm, 2.1 × 100 mm) in a gradient elution way with a mobile phase consisting of acetonitrile and water containing 0.1% formic acid at a flow rate of 0.4 mL/min. The plasma samples were prepared by a liquid⁻liquid extraction (LLE) method with ethyl acetate. The analytes were detected with a triple quadrupole tandem mass spectrometry (MS) in multiple reaction monitoring (MRM) mode and a positive ion electrospray ionization (ESI) of the transitions at m/z 432.4→414.4 for peimine and m/z 430.3→412.3 for peiminine. The method was linear for two analytes over the investigated range with all determined correlation coefficients exceeding 0.9900. The lower limit of quantification (LLOQ) was 0.988 ng/mL for peimine and 0.980 ng/mL for peiminine. The mean extraction recoveries of peimine and peiminine at three quality control samples (QC) levels were ranged from 82.56 to 88.71%, and matrix effects ranged from 92.06 to 101.2%. The intra-day and inter-day precision and accuracy were within the acceptable limits at LLOQ and QC levels. The method was effectively and successfully applied to the pharmacokinetics of peimine and peiminine after oral administration of powder to beagle dogs. The obtained results may be help to guide the clinical application of Fritillaria ussuriensis Maxim and Fritillaria thunbergii Miq. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/29958456/Simultaneous_Determination_and_Pharmacokinetics_of_Peimine_and_Peiminine_in_Beagle_Dog_Plasma_by_UPLC_MS/MS_after_the_Oral_Administration_of_Fritillariae_ussuriensis_Maxim_and_Fritillariae_thunbergii_Miq_Powder_ L2 - http://www.mdpi.com/resolver?pii=molecules23071573 DB - PRIME DP - Unbound Medicine ER -