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Cobalamin-specific R binder in pernicious anemia gastric juice: production by digestive enzyme action on saliva R binder.
Am J Gastroenterol. 1985 Nov; 80(11):841-52.AJ

Abstract

We found in four of five pernicious anemia gastric juices a partly degraded R binder which was cobalamin specific and has an apparent molecular weight of 60-70,000 daltons. Twenty-nine to 74% (4.8-27.0 ng/ml) of the corrinoid binding capacity could not be blocked by cobinamide (a noncobalamin corrinoid). The fifth pernicious anemia gastric juice and five nonpernicious anemia gastric juices had minimal amounts of this binder (2.5 and 2.2 +/- 1.4 ng/ml). Scatchard analysis revealed that cobalamin-specific R binder has 1000-fold lower affinity for cobinamide than cobalamin. Increasing quantities of trypsin and/or chymotrypsin digested increasing amounts of saliva R binder and an increasing percentage of the remaining digest-resistant R binder acquired cobalamin specificity. Partly degraded R binder in pernicious anemia gastric juice was resistant to further proteolysis. Cobalamin-specific R binder, perhaps produced in vivo by the action of refluxed pancreatic enzymes on swallowed R would preferentially bind ingested and/or biliary cobalamin rather than analogue and thereby could play a role in hastening the development of cobalamin deficiency in pernicious anemia.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2996328

Citation

Herzlich, B C., and V Herbert. "Cobalamin-specific R Binder in Pernicious Anemia Gastric Juice: Production By Digestive Enzyme Action On Saliva R Binder." The American Journal of Gastroenterology, vol. 80, no. 11, 1985, pp. 841-52.
Herzlich BC, Herbert V. Cobalamin-specific R binder in pernicious anemia gastric juice: production by digestive enzyme action on saliva R binder. Am J Gastroenterol. 1985;80(11):841-52.
Herzlich, B. C., & Herbert, V. (1985). Cobalamin-specific R binder in pernicious anemia gastric juice: production by digestive enzyme action on saliva R binder. The American Journal of Gastroenterology, 80(11), 841-52.
Herzlich BC, Herbert V. Cobalamin-specific R Binder in Pernicious Anemia Gastric Juice: Production By Digestive Enzyme Action On Saliva R Binder. Am J Gastroenterol. 1985;80(11):841-52. PubMed PMID: 2996328.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cobalamin-specific R binder in pernicious anemia gastric juice: production by digestive enzyme action on saliva R binder. AU - Herzlich,B C, AU - Herbert,V, PY - 1985/11/1/pubmed PY - 1985/11/1/medline PY - 1985/11/1/entrez SP - 841 EP - 52 JF - The American journal of gastroenterology JO - Am. J. Gastroenterol. VL - 80 IS - 11 N2 - We found in four of five pernicious anemia gastric juices a partly degraded R binder which was cobalamin specific and has an apparent molecular weight of 60-70,000 daltons. Twenty-nine to 74% (4.8-27.0 ng/ml) of the corrinoid binding capacity could not be blocked by cobinamide (a noncobalamin corrinoid). The fifth pernicious anemia gastric juice and five nonpernicious anemia gastric juices had minimal amounts of this binder (2.5 and 2.2 +/- 1.4 ng/ml). Scatchard analysis revealed that cobalamin-specific R binder has 1000-fold lower affinity for cobinamide than cobalamin. Increasing quantities of trypsin and/or chymotrypsin digested increasing amounts of saliva R binder and an increasing percentage of the remaining digest-resistant R binder acquired cobalamin specificity. Partly degraded R binder in pernicious anemia gastric juice was resistant to further proteolysis. Cobalamin-specific R binder, perhaps produced in vivo by the action of refluxed pancreatic enzymes on swallowed R would preferentially bind ingested and/or biliary cobalamin rather than analogue and thereby could play a role in hastening the development of cobalamin deficiency in pernicious anemia. SN - 0002-9270 UR - https://www.unboundmedicine.com/medline/citation/2996328/Cobalamin_specific_R_binder_in_pernicious_anemia_gastric_juice:_production_by_digestive_enzyme_action_on_saliva_R_binder_ L2 - http://www.diseaseinfosearch.org/result/441 DB - PRIME DP - Unbound Medicine ER -