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Ghrelin stimulates intestinal adaptation following massive small bowel resection in parenterally fed rats.
Peptides. 2018 Aug; 106:59-67.P

Abstract

BACKGROUND

Since short bowel syndrome (SBS) patients face life-threatening conditions, the development of therapeutic strategies to induce intestinal adaptation has been investigated. Ghrelin, a ligand of growth hormone (GH) secretagogue-receptor that stimulates the release of GH and insulin like growth factor-1 (IGF-1), has several pleiotropic effects. We investigated whether ghrelin induces intestinal adaptation in parenterally fed rats with SBS.

METHODS

Sprague-Dawley rats underwent venous catheterization and were divided into 3 groups: those receiving 90% small bowel resection while leaving the proximal jejunum and distal ileum (90% SBR) with TPN (SBS/TPN group), those receiving 90% SBR with TPN + ghrelin (SBS/TPN/ghrelin group), and those receiving sham operation and fed chow (sham group). Ghrelin was administered intravenously at 10 μg/kg/day. On Day 13, the rats were euthanized and the small intestine harvested, and the histology and crypt cell proliferation rates (CCPR), apoptosis, and nutrient transporter protein levels were analyzed and the plasma hormones were measured.

RESULTS

The villus height and crypt depth of the ileum in the SBS/TPN/ghrelin group were significantly higher than in the SBS/TPN group. The CCPR of the jejunum and the ileum significantly increased by the administration of ghrelin; however, the apoptosis rates did not significantly differ between the SBS/TPN and SBS/TPN/ghrelin groups. Significant differences did not exist in the plasma IGF-1 and nutrient transporter protein levels among three groups.

CONCLUSIONS

The intravenous administration of ghrelin stimulated the morphological intestinal adaptation of the ileum to a greater degree than the jejunum due to the direct effect of ghrelin.

Authors+Show Affiliations

Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Japan.Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Japan.Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Japan.Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Japan.Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Japan.Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Japan.Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Japan.Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Japan.Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Japan.Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Japan.Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Japan.Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Japan.Department of Pathology, Graduate School of Medical and Dental Sciences, Kagoshima University, Japan.Department of Pathology, Graduate School of Medical and Dental Sciences, Kagoshima University, Japan; Department of Pathology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.National Center for Children Health and Development, Pathology, Japan.Department of Pathology, Graduate School of Medical and Dental Sciences, Kagoshima University, Japan.Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Japan. Electronic address: sieiri@m.kufm.kagoshima-u.ac.jp.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29966680

Citation

Onishi, Shun, et al. "Ghrelin Stimulates Intestinal Adaptation Following Massive Small Bowel Resection in Parenterally Fed Rats." Peptides, vol. 106, 2018, pp. 59-67.
Onishi S, Kaji T, Yamada W, et al. Ghrelin stimulates intestinal adaptation following massive small bowel resection in parenterally fed rats. Peptides. 2018;106:59-67.
Onishi, S., Kaji, T., Yamada, W., Nakame, K., Machigashira, S., Kawano, M., Yano, K., Harumatsu, T., Yamada, K., Masuya, R., Kawano, T., Mukai, M., Hamada, T., Souda, M., Yoshioka, T., Tanimoto, A., & Ieiri, S. (2018). Ghrelin stimulates intestinal adaptation following massive small bowel resection in parenterally fed rats. Peptides, 106, 59-67. https://doi.org/10.1016/j.peptides.2018.06.009
Onishi S, et al. Ghrelin Stimulates Intestinal Adaptation Following Massive Small Bowel Resection in Parenterally Fed Rats. Peptides. 2018;106:59-67. PubMed PMID: 29966680.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ghrelin stimulates intestinal adaptation following massive small bowel resection in parenterally fed rats. AU - Onishi,Shun, AU - Kaji,Tatsuru, AU - Yamada,Waka, AU - Nakame,Kazuhiko, AU - Machigashira,Seiro, AU - Kawano,Masato, AU - Yano,Keisuke, AU - Harumatsu,Toshio, AU - Yamada,Koji, AU - Masuya,Ryuta, AU - Kawano,Takafumi, AU - Mukai,Motoi, AU - Hamada,Taiji, AU - Souda,Masakazu, AU - Yoshioka,Takako, AU - Tanimoto,Akihide, AU - Ieiri,Satoshi, Y1 - 2018/06/30/ PY - 2018/2/26/received PY - 2018/6/25/revised PY - 2018/6/26/accepted PY - 2018/7/4/pubmed PY - 2019/7/10/medline PY - 2018/7/4/entrez KW - GLP-2 KW - Gastrointestinal hormone KW - Ghrelin KW - IGF-1 KW - Intestinal adaptation KW - Massive bowel eresection KW - Parenteral nutrition KW - Parenteral nutrition-associated liver disease KW - Pediatric surgery KW - Short bowel syndrome SP - 59 EP - 67 JF - Peptides JO - Peptides VL - 106 N2 - BACKGROUND: Since short bowel syndrome (SBS) patients face life-threatening conditions, the development of therapeutic strategies to induce intestinal adaptation has been investigated. Ghrelin, a ligand of growth hormone (GH) secretagogue-receptor that stimulates the release of GH and insulin like growth factor-1 (IGF-1), has several pleiotropic effects. We investigated whether ghrelin induces intestinal adaptation in parenterally fed rats with SBS. METHODS: Sprague-Dawley rats underwent venous catheterization and were divided into 3 groups: those receiving 90% small bowel resection while leaving the proximal jejunum and distal ileum (90% SBR) with TPN (SBS/TPN group), those receiving 90% SBR with TPN + ghrelin (SBS/TPN/ghrelin group), and those receiving sham operation and fed chow (sham group). Ghrelin was administered intravenously at 10 μg/kg/day. On Day 13, the rats were euthanized and the small intestine harvested, and the histology and crypt cell proliferation rates (CCPR), apoptosis, and nutrient transporter protein levels were analyzed and the plasma hormones were measured. RESULTS: The villus height and crypt depth of the ileum in the SBS/TPN/ghrelin group were significantly higher than in the SBS/TPN group. The CCPR of the jejunum and the ileum significantly increased by the administration of ghrelin; however, the apoptosis rates did not significantly differ between the SBS/TPN and SBS/TPN/ghrelin groups. Significant differences did not exist in the plasma IGF-1 and nutrient transporter protein levels among three groups. CONCLUSIONS: The intravenous administration of ghrelin stimulated the morphological intestinal adaptation of the ileum to a greater degree than the jejunum due to the direct effect of ghrelin. SN - 1873-5169 UR - https://www.unboundmedicine.com/medline/citation/29966680/Ghrelin_stimulates_intestinal_adaptation_following_massive_small_bowel_resection_in_parenterally_fed_rats_ DB - PRIME DP - Unbound Medicine ER -