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Pm61: a recessive gene for resistance to powdery mildew in wheat landrace Xuxusanyuehuang identified by comparative genomics analysis.
Theor Appl Genet. 2018 Oct; 131(10):2085-2097.TA

Abstract

KEY MESSAGE

A single recessive powdery mildew resistance gene Pm61 from wheat landrace Xuxusanyuehuang was mapped within a 0.46-cM genetic interval spanning a 1.3-Mb interval of the genomic region of chromosome arm 4AL. Epidemics of powdery mildew incited by the biotrophic fungus Blumeria graminis f. sp. tritici (Bgt) have caused significant yield reductions in many wheat (Triticum aestivum)-producing regions. Identification of powdery mildew resistance genes is required for sustainable improvement of wheat for disease resistance. Chinese wheat landrace Xuxusanyuehuang was resistant to several Bgt isolates at the seedling stage. Genetic analysis based on the inoculation of Bgt isolate E09 on the F1, F2, and F2:3 populations produced by crossing Xuxusanyuehuang to susceptible cultivar Mingxian 169 revealed that the resistance of Xuxusanyuehuang was controlled by a single recessive gene. Bulked segregant analysis and simple sequence repeat (SSR) mapping placed the gene on chromosome bin 4AL-4-0.80-1.00. Comparative genomics analysis was performed to detect the collinear genomic regions of Brachypodium distachyon, rice, sorghum, Aegilops tauschii, T. urartu, and T. turgidum ssp. dicoccoides. Based on the use of 454 contig sequences and the International Wheat Genome Sequence Consortium survey sequence of Chinese Spring wheat, four EST-SSR and seven SSR markers were linked to the gene. An F5 recombinant inbred line population derived from Xuxusanyuehuang × Mingxian 169 cross was used to develop the genetic linkage map. The gene was localized in a 0.46-cM genetic interval between Xgwm160 and Xicsx79 corresponding to 1.3-Mb interval of the genomic region in wheat genome. This is a new locus for powdery mildew resistance on chromosome arm 4AL and is designated Pm61.

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Sun H
The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. College of Life and Environmental Science, Minzu University of China, Beijing, 100081, China.
Hu J
The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Song W
The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. College of Biology, Hunan University, Changsha, 410082, China.
Qiu D
The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Cui L
Institute of Crop Science, Shanxi Academy of Agricultural Sciences, Taiyuan, 030031, China.
Wu P
The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Zhang H
The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Liu H
The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Yang L
The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Qu Y
The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. College of Life Science and Technology, Harbin Normal University, Harbin, 150080, China.
Li Y
The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Li T
The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Cheng W
College of Chemistry and Environment Engineering, Pingdingshan University, Pingdingshan, 467000, China.
Zhou Y
The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Liu Z
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China. zyliu@genetics.ac.cn.
Li J
College of Chemistry and Environment Engineering, Pingdingshan University, Pingdingshan, 467000, China. jingting_lee@163.com.
Li H
The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. lihongjie@caas.cn.

MeSH

AscomycotaChromosome MappingComparative Genomic HybridizationDNA, PlantDisease ResistanceExpressed Sequence TagsGenes, PlantGenes, RecessiveGenetic LinkageGenetic MarkersMicrosatellite RepeatsPlant DiseasesTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29967989

Citation

Sun, Huigai, et al. "Pm61: a Recessive Gene for Resistance to Powdery Mildew in Wheat Landrace Xuxusanyuehuang Identified By Comparative Genomics Analysis." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 131, no. 10, 2018, pp. 2085-2097.
Sun H, Hu J, Song W, et al. Pm61: a recessive gene for resistance to powdery mildew in wheat landrace Xuxusanyuehuang identified by comparative genomics analysis. Theor Appl Genet. 2018;131(10):2085-2097.
Sun, H., Hu, J., Song, W., Qiu, D., Cui, L., Wu, P., Zhang, H., Liu, H., Yang, L., Qu, Y., Li, Y., Li, T., Cheng, W., Zhou, Y., Liu, Z., Li, J., & Li, H. (2018). Pm61: a recessive gene for resistance to powdery mildew in wheat landrace Xuxusanyuehuang identified by comparative genomics analysis. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 131(10), 2085-2097. https://doi.org/10.1007/s00122-018-3135-1
Sun H, et al. Pm61: a Recessive Gene for Resistance to Powdery Mildew in Wheat Landrace Xuxusanyuehuang Identified By Comparative Genomics Analysis. Theor Appl Genet. 2018;131(10):2085-2097. PubMed PMID: 29967989.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pm61: a recessive gene for resistance to powdery mildew in wheat landrace Xuxusanyuehuang identified by comparative genomics analysis. AU - Sun,Huigai, AU - Hu,Jinghuang, AU - Song,Wei, AU - Qiu,Dan, AU - Cui,Lei, AU - Wu,Peipei, AU - Zhang,Hongjun, AU - Liu,Hongwei, AU - Yang,Li, AU - Qu,Yunfeng, AU - Li,Yahui, AU - Li,Teng, AU - Cheng,Wei, AU - Zhou,Yang, AU - Liu,Zhiyong, AU - Li,Jingting, AU - Li,Hongjie, Y1 - 2018/07/02/ PY - 2018/02/16/received PY - 2018/06/21/accepted PY - 2018/7/4/pubmed PY - 2018/9/21/medline PY - 2018/7/4/entrez SP - 2085 EP - 2097 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 131 IS - 10 N2 - KEY MESSAGE: A single recessive powdery mildew resistance gene Pm61 from wheat landrace Xuxusanyuehuang was mapped within a 0.46-cM genetic interval spanning a 1.3-Mb interval of the genomic region of chromosome arm 4AL. Epidemics of powdery mildew incited by the biotrophic fungus Blumeria graminis f. sp. tritici (Bgt) have caused significant yield reductions in many wheat (Triticum aestivum)-producing regions. Identification of powdery mildew resistance genes is required for sustainable improvement of wheat for disease resistance. Chinese wheat landrace Xuxusanyuehuang was resistant to several Bgt isolates at the seedling stage. Genetic analysis based on the inoculation of Bgt isolate E09 on the F1, F2, and F2:3 populations produced by crossing Xuxusanyuehuang to susceptible cultivar Mingxian 169 revealed that the resistance of Xuxusanyuehuang was controlled by a single recessive gene. Bulked segregant analysis and simple sequence repeat (SSR) mapping placed the gene on chromosome bin 4AL-4-0.80-1.00. Comparative genomics analysis was performed to detect the collinear genomic regions of Brachypodium distachyon, rice, sorghum, Aegilops tauschii, T. urartu, and T. turgidum ssp. dicoccoides. Based on the use of 454 contig sequences and the International Wheat Genome Sequence Consortium survey sequence of Chinese Spring wheat, four EST-SSR and seven SSR markers were linked to the gene. An F5 recombinant inbred line population derived from Xuxusanyuehuang × Mingxian 169 cross was used to develop the genetic linkage map. The gene was localized in a 0.46-cM genetic interval between Xgwm160 and Xicsx79 corresponding to 1.3-Mb interval of the genomic region in wheat genome. This is a new locus for powdery mildew resistance on chromosome arm 4AL and is designated Pm61. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/29967989/Pm61:_a_recessive_gene_for_resistance_to_powdery_mildew_in_wheat_landrace_Xuxusanyuehuang_identified_by_comparative_genomics_analysis_ L2 - https://dx.doi.org/10.1007/s00122-018-3135-1 DB - PRIME DP - Unbound Medicine ER -