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Oleanolic acid attenuates TGF-β1-induced epithelial-mesenchymal transition in NRK-52E cells.
BMC Complement Altern Med. 2018 Jul 04; 18(1):205.BC

Abstract

BACKGROUND

Epithelial-to-mesenchymal transition (EMT) plays an important role in the progression of renal interstitial fibrosis, which finally leads to renal failure. Oleanolic acid (OA), an activator of NF-E2-related factor 2 (Nrf2), is reported to attenuate renal fibrosis in mice with unilateral ureteral obstruction. However, the role of OA in the regulation of EMT and the underlying mechanisms remain to be investigated. This study aimed to evaluate the effects of OA on EMT of renal proximal tubular epithelial cell line (NRK-52E) induced by TGF-β1, and to elucidate its underlying mechanism.

METHODS

Cells were incubated with TGF-β1 in the presence or absence of OA. The epithelial marker E-cadherin, the mesenchymal markers, α-smooth muscle actin (α-SMA), fibronectin, Nrf2, klotho, the signal transducer (p-Smad2/3), EMT initiator (Snail), and ILK were assayed by western blotting.

RESULTS

Our results showed that the NRK-52E cells incubated with TGF-β1 induced EMT with transition to the spindle-like morphology, down-regulated the expression of E-cadherin but up-regulated the expression of α-SMA and fibronectin. However, the treatment with OA reversed all EMT markers in a dose-dependent manner. OA also restored the expression of Nrf2 and klotho, decreased the phosphorylation of Smad2/3, ILK, and Snail in cells which was initiated by TGF-β1.

CONCLUSION

OA can attenuate TGF-β1 mediate EMT in renal tubular epithelial cells and may be a promising therapeutic agent in the treatment of renal fibrosis.

Authors+Show Affiliations

Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, Jiangsu, China.The First Affiliated Hospital of Soochow University, Suzhou, 215006, Jiangsu, China.Suzhou Hospital of Traditional Chinese Medicine, Suzhou, 215006, Jiangsu, China.Suzhou Municipal Hospital, Affiliated Hospital of Nanjing Medical University, Suzhou, 215006, Jiangsu, China.The First Affiliated Hospital of Soochow University, Suzhou, 215006, Jiangsu, China.The First Affiliated Hospital of Soochow University, Suzhou, 215006, Jiangsu, China.The First Affiliated Hospital of Soochow University, Suzhou, 215006, Jiangsu, China.The First Affiliated Hospital of Soochow University, Suzhou, 215006, Jiangsu, China.Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, Jiangsu, China.The First Affiliated Hospital of Soochow University, Suzhou, 215006, Jiangsu, China. weiminggang@suda.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29973206

Citation

He, Wei-Ming, et al. "Oleanolic Acid Attenuates TGF-β1-induced Epithelial-mesenchymal Transition in NRK-52E Cells." BMC Complementary and Alternative Medicine, vol. 18, no. 1, 2018, p. 205.
He WM, Yin JQ, Cheng XD, et al. Oleanolic acid attenuates TGF-β1-induced epithelial-mesenchymal transition in NRK-52E cells. BMC Complement Altern Med. 2018;18(1):205.
He, W. M., Yin, J. Q., Cheng, X. D., Lu, X., Ni, L., Xi, Y., Yin, G. D., Lu, G. Y., Sun, W., & Wei, M. G. (2018). Oleanolic acid attenuates TGF-β1-induced epithelial-mesenchymal transition in NRK-52E cells. BMC Complementary and Alternative Medicine, 18(1), 205. https://doi.org/10.1186/s12906-018-2265-y
He WM, et al. Oleanolic Acid Attenuates TGF-β1-induced Epithelial-mesenchymal Transition in NRK-52E Cells. BMC Complement Altern Med. 2018 Jul 4;18(1):205. PubMed PMID: 29973206.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oleanolic acid attenuates TGF-β1-induced epithelial-mesenchymal transition in NRK-52E cells. AU - He,Wei-Ming, AU - Yin,Jia-Qi, AU - Cheng,Xu-Dong, AU - Lu,Xun, AU - Ni,Li, AU - Xi,Yi, AU - Yin,Gui-Dong, AU - Lu,Guo-Yuan, AU - Sun,Wei, AU - Wei,Ming-Gang, Y1 - 2018/07/04/ PY - 2018/04/10/received PY - 2018/06/22/accepted PY - 2018/7/6/entrez PY - 2018/7/6/pubmed PY - 2018/7/31/medline KW - EMT KW - Klotho KW - Nrf2 KW - Oleanolic acid KW - TGF-β1 SP - 205 EP - 205 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 18 IS - 1 N2 - BACKGROUND: Epithelial-to-mesenchymal transition (EMT) plays an important role in the progression of renal interstitial fibrosis, which finally leads to renal failure. Oleanolic acid (OA), an activator of NF-E2-related factor 2 (Nrf2), is reported to attenuate renal fibrosis in mice with unilateral ureteral obstruction. However, the role of OA in the regulation of EMT and the underlying mechanisms remain to be investigated. This study aimed to evaluate the effects of OA on EMT of renal proximal tubular epithelial cell line (NRK-52E) induced by TGF-β1, and to elucidate its underlying mechanism. METHODS: Cells were incubated with TGF-β1 in the presence or absence of OA. The epithelial marker E-cadherin, the mesenchymal markers, α-smooth muscle actin (α-SMA), fibronectin, Nrf2, klotho, the signal transducer (p-Smad2/3), EMT initiator (Snail), and ILK were assayed by western blotting. RESULTS: Our results showed that the NRK-52E cells incubated with TGF-β1 induced EMT with transition to the spindle-like morphology, down-regulated the expression of E-cadherin but up-regulated the expression of α-SMA and fibronectin. However, the treatment with OA reversed all EMT markers in a dose-dependent manner. OA also restored the expression of Nrf2 and klotho, decreased the phosphorylation of Smad2/3, ILK, and Snail in cells which was initiated by TGF-β1. CONCLUSION: OA can attenuate TGF-β1 mediate EMT in renal tubular epithelial cells and may be a promising therapeutic agent in the treatment of renal fibrosis. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/29973206/Oleanolic_acid_attenuates_TGF_β1_induced_epithelial_mesenchymal_transition_in_NRK_52E_cells_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-018-2265-y DB - PRIME DP - Unbound Medicine ER -