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Toward Confirmatory On-Site Real-Time Detection of Emerging Drugs Using Portable Ultrafast Capillary Electrophoresis Mass Spectrometry.
Methods Mol Biol. 2018; 1810:43-58.MM

Abstract

Currently, law enforcement agencies rely upon presumptive tests such as color tests (or spot tests) for on-site, real-time identification of forensic evidence, such as controlled substances. These tests are simple and easy to use and require no instrumentation. However, they are unreliable and have a large false positive rate. On the other hand, confirmatory tests are done in analytical laboratories using sophisticated instrumentation by expert analysts, and have lower false positive rates. However, they are bulky and impractical for on-site real-time analysis. To provide more accurate identification of forensic evidence on-site, in real-time, it is important to develop portable confirmatory instrumentation using information-rich technologies. Moreover, because the analysis of controlled substances could be complicated by the existence of various isomers (including optical isomers) it is desirable that the portable instruments have the capability to separate structural and optical isomers of the controlled substances, because scheduling is some times dependent upon which isomer is present. To this end, we have developed a portable ultrafast capillary electrophoresis (UFCE) system for the separation of controlled substances and their structural and optical isomers. The UFCE instrument has an integrated porous tip for facile interfacing with electrospray ionization mass spectrometry. The technique has been successfully applied to the analysis of mixtures of several controlled substances such as amphetamines, cathinones, nor-mephedrone, and pregabalin and their optical isomers in about a minute.

Authors+Show Affiliations

Department of Forensic Sciences, George Washington University, Washington, DC, USA. moini.mehdi@gmail.com.

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

29974416

Citation

Moini, Mehdi. "Toward Confirmatory On-Site Real-Time Detection of Emerging Drugs Using Portable Ultrafast Capillary Electrophoresis Mass Spectrometry." Methods in Molecular Biology (Clifton, N.J.), vol. 1810, 2018, pp. 43-58.
Moini M. Toward Confirmatory On-Site Real-Time Detection of Emerging Drugs Using Portable Ultrafast Capillary Electrophoresis Mass Spectrometry. Methods Mol Biol. 2018;1810:43-58.
Moini, M. (2018). Toward Confirmatory On-Site Real-Time Detection of Emerging Drugs Using Portable Ultrafast Capillary Electrophoresis Mass Spectrometry. Methods in Molecular Biology (Clifton, N.J.), 1810, 43-58. https://doi.org/10.1007/978-1-4939-8579-1_4
Moini M. Toward Confirmatory On-Site Real-Time Detection of Emerging Drugs Using Portable Ultrafast Capillary Electrophoresis Mass Spectrometry. Methods Mol Biol. 2018;1810:43-58. PubMed PMID: 29974416.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Toward Confirmatory On-Site Real-Time Detection of Emerging Drugs Using Portable Ultrafast Capillary Electrophoresis Mass Spectrometry. A1 - Moini,Mehdi, PY - 2018/7/6/entrez PY - 2018/7/6/pubmed PY - 2019/3/16/medline KW - Attomole sensitivity KW - Integrated electrospray ionization source KW - On-site real-time detection KW - Portable confirmatory technique for forensic analysis KW - Quick analysis of illicit drugs KW - Ultrafast capillary electrophoresis mass spectrometry SP - 43 EP - 58 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol. Biol. VL - 1810 N2 - Currently, law enforcement agencies rely upon presumptive tests such as color tests (or spot tests) for on-site, real-time identification of forensic evidence, such as controlled substances. These tests are simple and easy to use and require no instrumentation. However, they are unreliable and have a large false positive rate. On the other hand, confirmatory tests are done in analytical laboratories using sophisticated instrumentation by expert analysts, and have lower false positive rates. However, they are bulky and impractical for on-site real-time analysis. To provide more accurate identification of forensic evidence on-site, in real-time, it is important to develop portable confirmatory instrumentation using information-rich technologies. Moreover, because the analysis of controlled substances could be complicated by the existence of various isomers (including optical isomers) it is desirable that the portable instruments have the capability to separate structural and optical isomers of the controlled substances, because scheduling is some times dependent upon which isomer is present. To this end, we have developed a portable ultrafast capillary electrophoresis (UFCE) system for the separation of controlled substances and their structural and optical isomers. The UFCE instrument has an integrated porous tip for facile interfacing with electrospray ionization mass spectrometry. The technique has been successfully applied to the analysis of mixtures of several controlled substances such as amphetamines, cathinones, nor-mephedrone, and pregabalin and their optical isomers in about a minute. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/29974416/Toward_Confirmatory_On-Site_Real-Time_Detection_of_Emerging_Drugs_Using_Portable_Ultrafast_Capillary_Electrophoresis_Mass_Spectrometry L2 - https://dx.doi.org/10.1007/978-1-4939-8579-1_4 DB - PRIME DP - Unbound Medicine ER -