TY - JOUR T1 - The effect of astaxanthin and cadmium on rat erythrocyte G6PD, 6PGD, GR, and TrxR enzymes activities in vivo and on rat erythrocyte 6PGD enzyme activity in vitro. AU - Bayramoğlu Akkoyun,Mahire, AU - Bengü,A Şükrü, AU - Temel,Yusuf, AU - Akkoyun,H Turan, AU - Ekin,Suat, AU - Ciftci,Mehmet, Y1 - 2018/07/05/ PY - 2018/05/01/received PY - 2018/06/13/revised PY - 2018/06/15/accepted PY - 2018/7/6/pubmed PY - 2018/9/25/medline PY - 2018/7/6/entrez KW - 6PGD KW - G6PD KW - GR KW - TrxR KW - astaxanthin KW - cadmium SP - e22170 EP - e22170 JF - Journal of biochemical and molecular toxicology JO - J. Biochem. Mol. Toxicol. VL - 32 IS - 8 N2 - In this study, the effects of astaxanthin (AST) that belongs to carotenoid family and cadmium (Cd), which is an important heavy metal, on rat erythrocyte G6PD, 6PGD, GR, and TrxR enzyme activities in vivo and on rat erythrocyte 6PGD enzyme activity in vitro were studied. In in vitro studies, 6PGD enzyme was purified from rat erythrocytes with 2',5'-ADP Sepharose4B affinity chromatography. Results showed inhibition of enzyme by Cd at IC50 ; 346.5 μM value and increase of 6PGD enzyme activity by AST. In vivo studies showed an increase in G6PD, 6PGD, and GR enzyme activities (P ˃ 0.05) and no chance in TrxR enzyme activity by AST. Cd ion inhibited G6PD, 6PGD, and GR enzyme activities (P ˂ 0.05) and also decreased TrxR enzyme activity (P ˃ 0.05). AST + Cd group G6PD enzyme activity was statistically low compared with control group (P ˂ 0.05). 6PGD and TrxR enzyme activities decreased without statistical significance (P ˃ 0.05); however, GR enzyme activity increased statistically significantly (P ˂ 0.05). SN - 1099-0461 UR - https://www.unboundmedicine.com/medline/citation/29975440/The_effect_of_astaxanthin_and_cadmium_on_rat_erythrocyte_G6PD_6PGD_GR_and_TrxR_enzymes_activities_in_vivo_and_on_rat_erythrocyte_6PGD_enzyme_activity_in_vitro_ L2 - https://doi.org/10.1002/jbt.22170 DB - PRIME DP - Unbound Medicine ER -