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Insights into citric acid-induced cadmium tolerance and phytoremediation in Brassica juncea L.: Coordinated functions of metal chelation, antioxidant defense and glyoxalase systems.
Ecotoxicol Environ Saf. 2018 Jan; 147:990-1001.EE

Abstract

Cadmium (Cd) is a serious environmental threat because it accumulates in plants from soil and is subsequently transported into the food cycle. Increased Cd uptake in plants disrupts plant metabolism and hampers crop growth and development. Therefore, remediation of Cd from soil and enhancing plant tolerance to metal toxicity is vital. In the present study, we investigated the function of different doses of citric acid (CA) on Cd toxicity in terms of metal accumulation and stress tolerance in mustard (Brassica juncea L.). Brassica juncea seedlings (12-day-old) were treated with Cd (0.5mMCd and 1.0mM CdCl2) alone and in combination with CA (0.5mM and 1.0mM) in a semi-hydroponic medium for three days. Cadmium accumulation in the roots and shoots of the mustard seedlings increased in a dose-dependent manner and was higher in the roots. Increasing the Cd concentration led to reduced growth, biomass, water status, and chlorophyll (chl) content resulting from increased oxidative damage (elevated malondialdehyde, MDA content; hydrogen peroxide, H2O2 level; superoxide, O2•- generation; lipoxygenase, LOX activity; and methylglyoxal, MG content) and downregulating of the major enzymes of the antioxidant defense and glyoxalase systems. Under Cd stress, both doses of CA improved the growth of the plants by enhancing leaf relative water content (RWC) and chl content; reducing oxidative damage; enhancing the pool of ascorbate (AsA) and glutathione (GSH) and the activities of the antioxidant enzymes (ascorbate peroxidase, APX; monodehydroascorbate reductase, MDHAR; dehydroascorbate reductase, DHAR; glutathione reductase, GR; glutathione peroxidase, GPX; superoxide dismutase, SOD; catalase, CAT); improving the performance of the glyoxalase system (glyoxalase I, Gly I and glyoxalase II, Gly II activity); and increasing the phytochelatin (PC) content. Exogenous CA also increased the root and shoot Cd content and Cd translocation from the roots to the shoots in a dose-dependent manner. Our findings suggest that CA plays a dual role in mustard seedlings by increasing phytoremediation and enhancing stress tolerance through upregulating the antioxidant defense and glyoxalase systems.

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Authors+Show Affiliations

Mahmud JA
Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan; Department of Agroforestry and Environmental Science, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, Bangladesh.
Hasanuzzaman M
Department of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, Bangladesh. Electronic address: mhzsauag@yahoo.com.
Nahar K
Department of Agricultural Botany, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, Bangladesh.
Bhuyan MHMB
Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan; Citrus Research Station, Bangladesh Agricultural Research Institute, Jaintapur, Sylhet, Bangladesh.
Fujita M
Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan. Electronic address: fujita@ag.kagawa-u.ac.jp.

MeSH

AntioxidantsAscorbate PeroxidasesAscorbic AcidBiodegradation, EnvironmentalCadmiumCatalaseCitric AcidGlutathioneGlutathione PeroxidaseGlutathione ReductaseHydrogen PeroxideLactoylglutathione LyaseMalondialdehydeMustard PlantOxidation-ReductionOxidoreductasesPhytochelatinsPyruvaldehydeSeedlingsSuperoxide DismutaseThiolester Hydrolases

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29976011

Citation

Mahmud, Jubayer Al, et al. "Insights Into Citric Acid-induced Cadmium Tolerance and Phytoremediation in Brassica Juncea L.: Coordinated Functions of Metal Chelation, Antioxidant Defense and Glyoxalase Systems." Ecotoxicology and Environmental Safety, vol. 147, 2018, pp. 990-1001.
Mahmud JA, Hasanuzzaman M, Nahar K, et al. Insights into citric acid-induced cadmium tolerance and phytoremediation in Brassica juncea L.: Coordinated functions of metal chelation, antioxidant defense and glyoxalase systems. Ecotoxicol Environ Saf. 2018;147:990-1001.
Mahmud, J. A., Hasanuzzaman, M., Nahar, K., Bhuyan, M. H. M. B., & Fujita, M. (2018). Insights into citric acid-induced cadmium tolerance and phytoremediation in Brassica juncea L.: Coordinated functions of metal chelation, antioxidant defense and glyoxalase systems. Ecotoxicology and Environmental Safety, 147, 990-1001. https://doi.org/10.1016/j.ecoenv.2017.09.045
Mahmud JA, et al. Insights Into Citric Acid-induced Cadmium Tolerance and Phytoremediation in Brassica Juncea L.: Coordinated Functions of Metal Chelation, Antioxidant Defense and Glyoxalase Systems. Ecotoxicol Environ Saf. 2018;147:990-1001. PubMed PMID: 29976011.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Insights into citric acid-induced cadmium tolerance and phytoremediation in Brassica juncea L.: Coordinated functions of metal chelation, antioxidant defense and glyoxalase systems. AU - Mahmud,Jubayer Al, AU - Hasanuzzaman,Mirza, AU - Nahar,Kamrun, AU - Bhuyan,M H M Borhannuddin, AU - Fujita,Masayuki, Y1 - 2017/10/07/ PY - 2017/07/21/received PY - 2017/09/13/revised PY - 2017/09/16/accepted PY - 2018/7/7/entrez PY - 2018/7/7/pubmed PY - 2018/10/30/medline KW - Abiotic stress KW - Antioxidant defense KW - Metal toxicity KW - Methylglyoxal KW - Organic acid KW - Oxidative stress SP - 990 EP - 1001 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 147 N2 - Cadmium (Cd) is a serious environmental threat because it accumulates in plants from soil and is subsequently transported into the food cycle. Increased Cd uptake in plants disrupts plant metabolism and hampers crop growth and development. Therefore, remediation of Cd from soil and enhancing plant tolerance to metal toxicity is vital. In the present study, we investigated the function of different doses of citric acid (CA) on Cd toxicity in terms of metal accumulation and stress tolerance in mustard (Brassica juncea L.). Brassica juncea seedlings (12-day-old) were treated with Cd (0.5mMCd and 1.0mM CdCl2) alone and in combination with CA (0.5mM and 1.0mM) in a semi-hydroponic medium for three days. Cadmium accumulation in the roots and shoots of the mustard seedlings increased in a dose-dependent manner and was higher in the roots. Increasing the Cd concentration led to reduced growth, biomass, water status, and chlorophyll (chl) content resulting from increased oxidative damage (elevated malondialdehyde, MDA content; hydrogen peroxide, H2O2 level; superoxide, O2•- generation; lipoxygenase, LOX activity; and methylglyoxal, MG content) and downregulating of the major enzymes of the antioxidant defense and glyoxalase systems. Under Cd stress, both doses of CA improved the growth of the plants by enhancing leaf relative water content (RWC) and chl content; reducing oxidative damage; enhancing the pool of ascorbate (AsA) and glutathione (GSH) and the activities of the antioxidant enzymes (ascorbate peroxidase, APX; monodehydroascorbate reductase, MDHAR; dehydroascorbate reductase, DHAR; glutathione reductase, GR; glutathione peroxidase, GPX; superoxide dismutase, SOD; catalase, CAT); improving the performance of the glyoxalase system (glyoxalase I, Gly I and glyoxalase II, Gly II activity); and increasing the phytochelatin (PC) content. Exogenous CA also increased the root and shoot Cd content and Cd translocation from the roots to the shoots in a dose-dependent manner. Our findings suggest that CA plays a dual role in mustard seedlings by increasing phytoremediation and enhancing stress tolerance through upregulating the antioxidant defense and glyoxalase systems. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/29976011/Insights_into_citric_acid_induced_cadmium_tolerance_and_phytoremediation_in_Brassica_juncea_L_:_Coordinated_functions_of_metal_chelation_antioxidant_defense_and_glyoxalase_systems_ DB - PRIME DP - Unbound Medicine ER -