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A terbium-based metal-organic framework@gold nanoparticle system as a fluorometric probe for aptamer based determination of adenosine triphosphate.
Mikrochim Acta. 2018 07 05; 185(8):359.MA

Abstract

This study reports on a method for fluorometric aptasensing of adenosine triphosphate (ATP). It is based on the interaction of dispersed (red) and agglomerated (blue) gold nanoparticles (AuNPs) with a water-dispered terbium(III) based metal-organic framework (Tb-MOF). The dispersed AuNPs quench the emissions of the Tb-MOF, while the aggregated AuNPs have little effect. Under the condition of high salt concentration, the free aptamer against ATP does not stabilize the AuNPs against aggregation. This causes a color change from red to blue and weak quenching of the fluorescence of the Tb-MOF (with peaks at 489 nm and 544 nm after excitation at 290 nm). On addition of ATP, it will be bound by its aptamer to form a complex that is adsorbed on the AuNPs. This protects the AuNPs from salt-induced aggregation and the color (with a peak at 525 nm) remains red. The two fluorescence bands of the Tb-MOF are therefore suppressed by fluorescence resonance energy transfer (FRET) between Tb-MOF and the dispersed AuNPs. Fluorescence drops linearly in the 50 nM to 10 μM ATP concentration range, and the detection limit is 23 nM. ATP analogs such as guanosine triphosphate, uridine triphosphate, cytidine triphosphate, adenosine monophosphate and cyclic adenosine monophosphate have no obvious interference. The method was successfully applied to the determination of ATP in (spiked) human plasma samples and gave satisfactory recoveries. Graphical abstract Schematic of a terbium-based metal-organic framework@gold nanoparticle system as a fluorometric probe for aptamer based determination of adenosine triphosphate. The dispersed gold nanoparticles (AuNPs) quench the fluorescence of the terbium-based metal-organic framework (Tb-MOF), while the aggregated AuNPs have little effect. In the presence of adenosine triphosphate (ATP), the aptamer-ATP complexes provide greater protection towards AuNPs than aptamer alone under high salt condition. Based on this, a novel Tb-MOF@AuNP platform is established for ATP detection.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Qu F
The Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu, 273165, Shandong, China. qufei3323@163.com. The Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University, Qufu, 273165, Shandong, China. qufei3323@163.com.
Sun C
The Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu, 273165, Shandong, China. The Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University, Qufu, 273165, Shandong, China.
Lv X
The Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu, 273165, Shandong, China. The Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University, Qufu, 273165, Shandong, China.
You J
The Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu, 273165, Shandong, China. The Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University, Qufu, 273165, Shandong, China. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, China.

MeSH

Adenosine TriphosphateAptamers, NucleotideBiosensing TechniquesFluorescent DyesFluorometryGoldHumansLimit of DetectionMetal NanoparticlesMetal-Organic FrameworksModels, MolecularMolecular ConformationTerbium

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29978289

Citation

Qu, Fei, et al. "A Terbium-based Metal-organic Framework@gold Nanoparticle System as a Fluorometric Probe for Aptamer Based Determination of Adenosine Triphosphate." Mikrochimica Acta, vol. 185, no. 8, 2018, p. 359.
Qu F, Sun C, Lv X, et al. A terbium-based metal-organic framework@gold nanoparticle system as a fluorometric probe for aptamer based determination of adenosine triphosphate. Mikrochim Acta. 2018;185(8):359.
Qu, F., Sun, C., Lv, X., & You, J. (2018). A terbium-based metal-organic framework@gold nanoparticle system as a fluorometric probe for aptamer based determination of adenosine triphosphate. Mikrochimica Acta, 185(8), 359. https://doi.org/10.1007/s00604-018-2888-1
Qu F, et al. A Terbium-based Metal-organic Framework@gold Nanoparticle System as a Fluorometric Probe for Aptamer Based Determination of Adenosine Triphosphate. Mikrochim Acta. 2018 07 5;185(8):359. PubMed PMID: 29978289.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A terbium-based metal-organic framework@gold nanoparticle system as a fluorometric probe for aptamer based determination of adenosine triphosphate. AU - Qu,Fei, AU - Sun,Chao, AU - Lv,Xiaoxia, AU - You,Jinmao, Y1 - 2018/07/05/ PY - 2018/04/08/received PY - 2018/06/25/accepted PY - 2018/7/7/entrez PY - 2018/7/7/pubmed PY - 2019/2/6/medline KW - ATP KW - ATP-binding aptamer KW - Aggregation KW - Aptamer-ATP complex KW - Aptasensing KW - Biosensing KW - Dispersion KW - Gold nanoparticles KW - Lanthanide MOF KW - ssDNA SP - 359 EP - 359 JF - Mikrochimica acta JO - Mikrochim Acta VL - 185 IS - 8 N2 - This study reports on a method for fluorometric aptasensing of adenosine triphosphate (ATP). It is based on the interaction of dispersed (red) and agglomerated (blue) gold nanoparticles (AuNPs) with a water-dispered terbium(III) based metal-organic framework (Tb-MOF). The dispersed AuNPs quench the emissions of the Tb-MOF, while the aggregated AuNPs have little effect. Under the condition of high salt concentration, the free aptamer against ATP does not stabilize the AuNPs against aggregation. This causes a color change from red to blue and weak quenching of the fluorescence of the Tb-MOF (with peaks at 489 nm and 544 nm after excitation at 290 nm). On addition of ATP, it will be bound by its aptamer to form a complex that is adsorbed on the AuNPs. This protects the AuNPs from salt-induced aggregation and the color (with a peak at 525 nm) remains red. The two fluorescence bands of the Tb-MOF are therefore suppressed by fluorescence resonance energy transfer (FRET) between Tb-MOF and the dispersed AuNPs. Fluorescence drops linearly in the 50 nM to 10 μM ATP concentration range, and the detection limit is 23 nM. ATP analogs such as guanosine triphosphate, uridine triphosphate, cytidine triphosphate, adenosine monophosphate and cyclic adenosine monophosphate have no obvious interference. The method was successfully applied to the determination of ATP in (spiked) human plasma samples and gave satisfactory recoveries. Graphical abstract Schematic of a terbium-based metal-organic framework@gold nanoparticle system as a fluorometric probe for aptamer based determination of adenosine triphosphate. The dispersed gold nanoparticles (AuNPs) quench the fluorescence of the terbium-based metal-organic framework (Tb-MOF), while the aggregated AuNPs have little effect. In the presence of adenosine triphosphate (ATP), the aptamer-ATP complexes provide greater protection towards AuNPs than aptamer alone under high salt condition. Based on this, a novel Tb-MOF@AuNP platform is established for ATP detection. SN - 1436-5073 UR - https://www.unboundmedicine.com/medline/citation/29978289/A_terbium_based_metal_organic_framework@gold_nanoparticle_system_as_a_fluorometric_probe_for_aptamer_based_determination_of_adenosine_triphosphate_ DB - PRIME DP - Unbound Medicine ER -