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Effects of berberine on growth, liver histology, and expression of lipid-related genes in blunt snout bream (Megalobrama amblycephala) fed high-fat diets.
Fish Physiol Biochem 2019; 45(1):83-91FP

Abstract

Fatty liver of cultured fish often correlates closely with poor growth and low harvest yield. Some Chinese herbs can reduce hepatic fat storage. This study aimed to examine lipid-lowering effect of berberine (BBR) in blunt snout bream (Megalobrama amblycephala). Triplicate groups of fish were fed four experimental diets: low-fat diet (LFD, 5% fat), high-fat diet (HFD, 15% fat), and HFD supplemented with 50 or 100 mg BBR/kg diet (BBR50, BBR100). After 8-week feeding, growth performance, liver histology and fat deposition, and hepatic genes expression were examined. The results showed significant reduction of growth performance and feed intake in fish fed HFD compared to those fed the LFD and BBR50 diets. Supplementing 50 mg BBR/kg to the HFD significantly improved weight gain and feed intake. Higher hepatic fat content and histological abnormalities were found in the liver of fish receiving HFD, and BBR50 and BBR100 could attenuate these abnormalities of liver. Expression of CPT I, AOX, ApoB100, ApoE, and PGC-1α genes was significantly decreased in fish fed HFD, and 50 and 100 mg/kg BBR supplementation could revert the downregulation of these genes. Also, the expression of FATP, LPL, and LDLR genes was upregulated in HFD-fed fish, and their expression was significantly decreased by 50 and 100 mg/kg BBR supplementation. In conclusion, supplementing BBR to HFD could attenuate liver fat deposition and disorders. The fat-lowering effects of BBR appear to be mediated by activating genes related with fatty acid oxidation and decreasing genes for fatty acid uptake.

Authors+Show Affiliations

Fisheries College, Jimei University, Xiamen, 361021, China.Fisheries College, Jimei University, Xiamen, 361021, China.Fisheries College, Jimei University, Xiamen, 361021, China. lukangle@jmu.edu.cn.College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.

Pub Type(s)

Clinical Trial, Veterinary
Journal Article

Language

eng

PubMed ID

29984398

Citation

Zhou, Wenhao, et al. "Effects of Berberine On Growth, Liver Histology, and Expression of Lipid-related Genes in Blunt Snout Bream (Megalobrama Amblycephala) Fed High-fat Diets." Fish Physiology and Biochemistry, vol. 45, no. 1, 2019, pp. 83-91.
Zhou W, Rahimnejad S, Lu K, et al. Effects of berberine on growth, liver histology, and expression of lipid-related genes in blunt snout bream (Megalobrama amblycephala) fed high-fat diets. Fish Physiol Biochem. 2019;45(1):83-91.
Zhou, W., Rahimnejad, S., Lu, K., Wang, L., & Liu, W. (2019). Effects of berberine on growth, liver histology, and expression of lipid-related genes in blunt snout bream (Megalobrama amblycephala) fed high-fat diets. Fish Physiology and Biochemistry, 45(1), pp. 83-91. doi:10.1007/s10695-018-0536-7.
Zhou W, et al. Effects of Berberine On Growth, Liver Histology, and Expression of Lipid-related Genes in Blunt Snout Bream (Megalobrama Amblycephala) Fed High-fat Diets. Fish Physiol Biochem. 2019;45(1):83-91. PubMed PMID: 29984398.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of berberine on growth, liver histology, and expression of lipid-related genes in blunt snout bream (Megalobrama amblycephala) fed high-fat diets. AU - Zhou,Wenhao, AU - Rahimnejad,Samad, AU - Lu,Kangle, AU - Wang,Lina, AU - Liu,Wenbin, Y1 - 2018/07/08/ PY - 2017/06/02/received PY - 2018/07/02/accepted PY - 2018/7/10/pubmed PY - 2019/6/5/medline PY - 2018/7/10/entrez KW - Berberine KW - Fat deposition KW - Fatty liver KW - Fish KW - High-fat diet SP - 83 EP - 91 JF - Fish physiology and biochemistry JO - Fish Physiol. Biochem. VL - 45 IS - 1 N2 - Fatty liver of cultured fish often correlates closely with poor growth and low harvest yield. Some Chinese herbs can reduce hepatic fat storage. This study aimed to examine lipid-lowering effect of berberine (BBR) in blunt snout bream (Megalobrama amblycephala). Triplicate groups of fish were fed four experimental diets: low-fat diet (LFD, 5% fat), high-fat diet (HFD, 15% fat), and HFD supplemented with 50 or 100 mg BBR/kg diet (BBR50, BBR100). After 8-week feeding, growth performance, liver histology and fat deposition, and hepatic genes expression were examined. The results showed significant reduction of growth performance and feed intake in fish fed HFD compared to those fed the LFD and BBR50 diets. Supplementing 50 mg BBR/kg to the HFD significantly improved weight gain and feed intake. Higher hepatic fat content and histological abnormalities were found in the liver of fish receiving HFD, and BBR50 and BBR100 could attenuate these abnormalities of liver. Expression of CPT I, AOX, ApoB100, ApoE, and PGC-1α genes was significantly decreased in fish fed HFD, and 50 and 100 mg/kg BBR supplementation could revert the downregulation of these genes. Also, the expression of FATP, LPL, and LDLR genes was upregulated in HFD-fed fish, and their expression was significantly decreased by 50 and 100 mg/kg BBR supplementation. In conclusion, supplementing BBR to HFD could attenuate liver fat deposition and disorders. The fat-lowering effects of BBR appear to be mediated by activating genes related with fatty acid oxidation and decreasing genes for fatty acid uptake. SN - 1573-5168 UR - https://www.unboundmedicine.com/medline/citation/29984398/Effects_of_berberine_on_growth_liver_histology_and_expression_of_lipid_related_genes_in_blunt_snout_bream__Megalobrama_amblycephala__fed_high_fat_diets_ L2 - https://dx.doi.org/10.1007/s10695-018-0536-7 DB - PRIME DP - Unbound Medicine ER -