Tags

Type your tag names separated by a space and hit enter

Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy.
Nat Methods 2018; 15(8):601-604NM

Abstract

Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.

Authors+Show Affiliations

Department of Applied Physics and Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, USA.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.Department of Applied Physics and Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden.Department of Applied Physics and Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, USA.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation. mishin@ibch.ru.Department of Applied Physics and Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden. ilaria.testa@scilifelab.se.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29988095

Citation

Pennacchietti, Francesca, et al. "Fast Reversibly Photoswitching Red Fluorescent Proteins for Live-cell RESOLFT Nanoscopy." Nature Methods, vol. 15, no. 8, 2018, pp. 601-604.
Pennacchietti F, Serebrovskaya EO, Faro AR, et al. Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy. Nat Methods. 2018;15(8):601-604.
Pennacchietti, F., Serebrovskaya, E. O., Faro, A. R., Shemyakina, I. I., Bozhanova, N. G., Kotlobay, A. A., ... Testa, I. (2018). Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy. Nature Methods, 15(8), pp. 601-604. doi:10.1038/s41592-018-0052-9.
Pennacchietti F, et al. Fast Reversibly Photoswitching Red Fluorescent Proteins for Live-cell RESOLFT Nanoscopy. Nat Methods. 2018;15(8):601-604. PubMed PMID: 29988095.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy. AU - Pennacchietti,Francesca, AU - Serebrovskaya,Ekaterina O, AU - Faro,Aline R, AU - Shemyakina,Irina I, AU - Bozhanova,Nina G, AU - Kotlobay,Alexey A, AU - Gurskaya,Nadya G, AU - Bodén,Andreas, AU - Dreier,Jes, AU - Chudakov,Dmitry M, AU - Lukyanov,Konstantin A, AU - Verkhusha,Vladislav V, AU - Mishin,Alexander S, AU - Testa,Ilaria, Y1 - 2018/07/09/ PY - 2018/02/08/received PY - 2018/05/18/accepted PY - 2018/7/11/pubmed PY - 2019/5/29/medline PY - 2018/7/11/entrez SP - 601 EP - 604 JF - Nature methods JO - Nat. Methods VL - 15 IS - 8 N2 - Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells. SN - 1548-7105 UR - https://www.unboundmedicine.com/medline/citation/29988095/Fast_reversibly_photoswitching_red_fluorescent_proteins_for_live_cell_RESOLFT_nanoscopy_ L2 - http://dx.doi.org/10.1038/s41592-018-0052-9 DB - PRIME DP - Unbound Medicine ER -