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The aminoglycoside-resistance operon of the plasmid pSa: nucleotide sequence of the streptomycin-spectinomycin resistance gene.
Gene 1985; 36(1-2):97-104GENE

Abstract

The nucleotide sequence of the probable C terminus of the kanamycin-resistance gene (KmR) and the probable complete sequence of the streptomycin-spectinomycin-resistance gene (SpR) of the IncW plasmid pSa have been determined. The two genes appear to be oriented in the same direction and separated by a spacer region of 53 bp, with transcription proceeding from the KmR gene into the SpR gene. An RNA transcript encompassing the C terminus of the KmR gene, the 53-base spacer, and the N terminus of the SpR gene has the potential to form a stem-loop structure with a free energy value of -68 kcal/mol. The SpR gene of pSa has extensive sequence homology with the aadA gene of the plasmid R538-1. Comparison of the proposed amino acid sequence of the KmR protein of pSa with those of two aminoglycoside phosphotransferases revealed a region of potential homology with those proteins.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2998941

Citation

Tait, R C., et al. "The Aminoglycoside-resistance Operon of the Plasmid pSa: Nucleotide Sequence of the Streptomycin-spectinomycin Resistance Gene." Gene, vol. 36, no. 1-2, 1985, pp. 97-104.
Tait RC, Rempel H, Rodriguez RL, et al. The aminoglycoside-resistance operon of the plasmid pSa: nucleotide sequence of the streptomycin-spectinomycin resistance gene. Gene. 1985;36(1-2):97-104.
Tait, R. C., Rempel, H., Rodriguez, R. L., & Kado, C. I. (1985). The aminoglycoside-resistance operon of the plasmid pSa: nucleotide sequence of the streptomycin-spectinomycin resistance gene. Gene, 36(1-2), pp. 97-104.
Tait RC, et al. The Aminoglycoside-resistance Operon of the Plasmid pSa: Nucleotide Sequence of the Streptomycin-spectinomycin Resistance Gene. Gene. 1985;36(1-2):97-104. PubMed PMID: 2998941.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The aminoglycoside-resistance operon of the plasmid pSa: nucleotide sequence of the streptomycin-spectinomycin resistance gene. AU - Tait,R C, AU - Rempel,H, AU - Rodriguez,R L, AU - Kado,C I, PY - 1985/1/1/pubmed PY - 1985/1/1/medline PY - 1985/1/1/entrez SP - 97 EP - 104 JF - Gene JO - Gene VL - 36 IS - 1-2 N2 - The nucleotide sequence of the probable C terminus of the kanamycin-resistance gene (KmR) and the probable complete sequence of the streptomycin-spectinomycin-resistance gene (SpR) of the IncW plasmid pSa have been determined. The two genes appear to be oriented in the same direction and separated by a spacer region of 53 bp, with transcription proceeding from the KmR gene into the SpR gene. An RNA transcript encompassing the C terminus of the KmR gene, the 53-base spacer, and the N terminus of the SpR gene has the potential to form a stem-loop structure with a free energy value of -68 kcal/mol. The SpR gene of pSa has extensive sequence homology with the aadA gene of the plasmid R538-1. Comparison of the proposed amino acid sequence of the KmR protein of pSa with those of two aminoglycoside phosphotransferases revealed a region of potential homology with those proteins. SN - 0378-1119 UR - https://www.unboundmedicine.com/medline/citation/2998941/The_aminoglycoside_resistance_operon_of_the_plasmid_pSa:_nucleotide_sequence_of_the_streptomycin_spectinomycin_resistance_gene_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0378-1119(85)90073-3 DB - PRIME DP - Unbound Medicine ER -