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Requirement of Ia-positive accessory cells in the MLR response against class II antigen on human B cell tumor line.
J Immunol. 1985 Dec; 135(6):3887-96.JI

Abstract

In this report we have made a comparative study of the capacity of normal human stimulator cells and Epstein-Barr virus-transformed human B cell line Wa (EBV-Wa) cells to stimulate alloreactive T cells. Class II antigen (presumably HLA-DR4 determinant) on EBV-Wa cells was shown to act as a stimulating molecule in the mixed lymphocyte reaction (MLR) through a blocking study by using anti-Ia antibodies. Furthermore, it was found that HLA-DR-positive accessory cells in the responder population were required to elicit MLR responses against HLA-DR antigen on EBV-Wa cells. In contrast, HLA-DR-positive accessory cells in the responding cell population were not essential for elicitation of MLR responses against HLA-DR antigen on normal allogeneic peripheral blood mononuclear cells, as reported. The cell-cell interaction between responder HLA-DR-positive accessory cells and responding T cells in a major histocompatibility complex (MHC)-restricted manner was required for eliciting MLR responses against class II antigen on EBV-Wa cells such as antigen-presenting cell-T cell interaction in soluble antigen-specific T cell proliferative responses. The function of HLA-DR-positive accessory cells in the responder population could not be substituted for by the presence of interleukin 1. Furthermore, there was no obvious correlation between the degree of surface HLA-DR antigen expression on EBV-Wa cells and its stimulating ability. Thus, two distinct types of allo-class II, antigen-specific T cell activation between normal human stimulator cells and EBV-Wa cells were shown to exist.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2999233

Citation

Yamamoto, M, and A Yano. "Requirement of Ia-positive Accessory Cells in the MLR Response Against Class II Antigen On Human B Cell Tumor Line." Journal of Immunology (Baltimore, Md. : 1950), vol. 135, no. 6, 1985, pp. 3887-96.
Yamamoto M, Yano A. Requirement of Ia-positive accessory cells in the MLR response against class II antigen on human B cell tumor line. J Immunol. 1985;135(6):3887-96.
Yamamoto, M., & Yano, A. (1985). Requirement of Ia-positive accessory cells in the MLR response against class II antigen on human B cell tumor line. Journal of Immunology (Baltimore, Md. : 1950), 135(6), 3887-96.
Yamamoto M, Yano A. Requirement of Ia-positive Accessory Cells in the MLR Response Against Class II Antigen On Human B Cell Tumor Line. J Immunol. 1985;135(6):3887-96. PubMed PMID: 2999233.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Requirement of Ia-positive accessory cells in the MLR response against class II antigen on human B cell tumor line. AU - Yamamoto,M, AU - Yano,A, PY - 1985/12/1/pubmed PY - 1985/12/1/medline PY - 1985/12/1/entrez SP - 3887 EP - 96 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 135 IS - 6 N2 - In this report we have made a comparative study of the capacity of normal human stimulator cells and Epstein-Barr virus-transformed human B cell line Wa (EBV-Wa) cells to stimulate alloreactive T cells. Class II antigen (presumably HLA-DR4 determinant) on EBV-Wa cells was shown to act as a stimulating molecule in the mixed lymphocyte reaction (MLR) through a blocking study by using anti-Ia antibodies. Furthermore, it was found that HLA-DR-positive accessory cells in the responder population were required to elicit MLR responses against HLA-DR antigen on EBV-Wa cells. In contrast, HLA-DR-positive accessory cells in the responding cell population were not essential for elicitation of MLR responses against HLA-DR antigen on normal allogeneic peripheral blood mononuclear cells, as reported. The cell-cell interaction between responder HLA-DR-positive accessory cells and responding T cells in a major histocompatibility complex (MHC)-restricted manner was required for eliciting MLR responses against class II antigen on EBV-Wa cells such as antigen-presenting cell-T cell interaction in soluble antigen-specific T cell proliferative responses. The function of HLA-DR-positive accessory cells in the responder population could not be substituted for by the presence of interleukin 1. Furthermore, there was no obvious correlation between the degree of surface HLA-DR antigen expression on EBV-Wa cells and its stimulating ability. Thus, two distinct types of allo-class II, antigen-specific T cell activation between normal human stimulator cells and EBV-Wa cells were shown to exist. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2999233/Requirement_of_Ia_positive_accessory_cells_in_the_MLR_response_against_class_II_antigen_on_human_B_cell_tumor_line_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=2999233 DB - PRIME DP - Unbound Medicine ER -