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Influence of Extraction Conditions on Ultrasound-Assisted Recovery of Bioactive Phenolics from Blueberry Pomace and Their Antioxidant Activity.
Molecules. 2018 Jul 11; 23(7)M

Abstract

The increase in diet-related chronic diseases has prompted the search for health-promoting compounds and methods to ensure their quality. Blueberry pomace is a rich yet underutilized source of bioactive polyphenols. For these high-value bioactive molecules, ultrasound-assisted extraction (USAE) is an attractive and green alternative to conventional extraction techniques for improving purity and yields. This study aimed to assess the impact of USAE parameters (sonication time, solvent composition, solid/liquid ratio, pH and temperature) on the recovery of phenolic compounds from blueberry pomace and antioxidant activity of the extracts. Total phenolic, flavonoid and anthocyanin contents (TPC, TFC and TAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity were analysed. USAE in 50% ethanol/water was the most efficient, yielding the highest TPC (22.33 mg/g dry matter (DM)), TFC (19.41 mg/g DM), TAC (31.32 mg/g DM) and DPPH radical scavenging activity (41.79 mg Trolox/g DM). USAE in water showed the lowest values even at low (1/40) solid/liquid ratio (7.85 mg/g DM, 3.49 mg/g DM, and 18.96 mg/g DM for TPC, TFC and TAC, respectively). Decreasing the solid/liquid ratio in water or 50% ethanol significantly increased TPC, TFC, TAC and DPPH radical scavenging. With ethanol, increasing the temperature in the range 20⁻40 °C decreased TPC but increased TFC and DPPH radical scavenging activity. Anthocyanin profiles of water and ethanolic extracts were qualitatively similar, consisting of malvidin, delphinidin, petunidin and cyanidin. These findings indicate that USAE is a method of choice for extracting high-value bioactive phenolics from blueberry pomace. Selective enrichment of different phenolic fractions is possible under select extraction conditions.

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Authors+Show Affiliations

Bamba BSB
Department of Biochemistry and Genetics, Biological Sciences Training and Research Unit, Université Peleforo Gon Coulibaly, Korhogo BP 1328, Côte d'Ivoire. bamba_bio@upgc.edu.ci. Agriculture and Agri-Food Canada, Guelph Research and Development Centre, Guelph, ON N1G 5C9, Canada. bamba_bio@upgc.edu.ci. School of Food Science, Nutrition and Family Studies, Université de Moncton, Moncton, NB E1A 3E9, Canada. bamba_bio@upgc.edu.ci.
Shi J
Agriculture and Agri-Food Canada, Guelph Research and Development Centre, Guelph, ON N1G 5C9, Canada. john.shi@agr.gc.ca.
Tranchant CC
School of Food Science, Nutrition and Family Studies, Université de Moncton, Moncton, NB E1A 3E9, Canada. carole.tranchant@umoncton.ca.
Xue SJ
Agriculture and Agri-Food Canada, Guelph Research and Development Centre, Guelph, ON N1G 5C9, Canada. jun.xue@agr.gc.ca.
Forney CF
Agriculture and Agri-Food Canada, Kentville Research and Development Centre, Kentville, NS B4N 1J5, Canada. charles.forney@agr.gc.ca.
Lim LT
Food Science Department, University of Guelph, Guelph, ON N1G 2W1, Canada. llim@uoguelph.ca.

MeSH

AntioxidantsBlueberry PlantsChromatography, High Pressure LiquidEthanolHydrogen-Ion ConcentrationPhenolsPlant ExtractsReference StandardsSolventsSonicationTemperatureTime FactorsUltrasonicsWater

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29997308

Citation

Bamba, Bio Sigui Bruno, et al. "Influence of Extraction Conditions On Ultrasound-Assisted Recovery of Bioactive Phenolics From Blueberry Pomace and Their Antioxidant Activity." Molecules (Basel, Switzerland), vol. 23, no. 7, 2018.
Bamba BSB, Shi J, Tranchant CC, et al. Influence of Extraction Conditions on Ultrasound-Assisted Recovery of Bioactive Phenolics from Blueberry Pomace and Their Antioxidant Activity. Molecules. 2018;23(7).
Bamba, B. S. B., Shi, J., Tranchant, C. C., Xue, S. J., Forney, C. F., & Lim, L. T. (2018). Influence of Extraction Conditions on Ultrasound-Assisted Recovery of Bioactive Phenolics from Blueberry Pomace and Their Antioxidant Activity. Molecules (Basel, Switzerland), 23(7). https://doi.org/10.3390/molecules23071685
Bamba BSB, et al. Influence of Extraction Conditions On Ultrasound-Assisted Recovery of Bioactive Phenolics From Blueberry Pomace and Their Antioxidant Activity. Molecules. 2018 Jul 11;23(7) PubMed PMID: 29997308.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of Extraction Conditions on Ultrasound-Assisted Recovery of Bioactive Phenolics from Blueberry Pomace and Their Antioxidant Activity. AU - Bamba,Bio Sigui Bruno, AU - Shi,John, AU - Tranchant,Carole C, AU - Xue,Sophia Jun, AU - Forney,Charles F, AU - Lim,Loong-Tak, Y1 - 2018/07/11/ PY - 2018/06/28/received PY - 2018/07/08/revised PY - 2018/07/09/accepted PY - 2018/7/13/entrez PY - 2018/7/13/pubmed PY - 2018/10/23/medline KW - anthocyanins KW - antioxidant activity KW - blueberry pomace KW - extraction parameters KW - flavonoids KW - green technology KW - polyphenols KW - ultrasound-assisted extraction JF - Molecules (Basel, Switzerland) JO - Molecules VL - 23 IS - 7 N2 - The increase in diet-related chronic diseases has prompted the search for health-promoting compounds and methods to ensure their quality. Blueberry pomace is a rich yet underutilized source of bioactive polyphenols. For these high-value bioactive molecules, ultrasound-assisted extraction (USAE) is an attractive and green alternative to conventional extraction techniques for improving purity and yields. This study aimed to assess the impact of USAE parameters (sonication time, solvent composition, solid/liquid ratio, pH and temperature) on the recovery of phenolic compounds from blueberry pomace and antioxidant activity of the extracts. Total phenolic, flavonoid and anthocyanin contents (TPC, TFC and TAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity were analysed. USAE in 50% ethanol/water was the most efficient, yielding the highest TPC (22.33 mg/g dry matter (DM)), TFC (19.41 mg/g DM), TAC (31.32 mg/g DM) and DPPH radical scavenging activity (41.79 mg Trolox/g DM). USAE in water showed the lowest values even at low (1/40) solid/liquid ratio (7.85 mg/g DM, 3.49 mg/g DM, and 18.96 mg/g DM for TPC, TFC and TAC, respectively). Decreasing the solid/liquid ratio in water or 50% ethanol significantly increased TPC, TFC, TAC and DPPH radical scavenging. With ethanol, increasing the temperature in the range 20⁻40 °C decreased TPC but increased TFC and DPPH radical scavenging activity. Anthocyanin profiles of water and ethanolic extracts were qualitatively similar, consisting of malvidin, delphinidin, petunidin and cyanidin. These findings indicate that USAE is a method of choice for extracting high-value bioactive phenolics from blueberry pomace. Selective enrichment of different phenolic fractions is possible under select extraction conditions. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/29997308/Influence_of_Extraction_Conditions_on_Ultrasound_Assisted_Recovery_of_Bioactive_Phenolics_from_Blueberry_Pomace_and_Their_Antioxidant_Activity_ DB - PRIME DP - Unbound Medicine ER -