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Enhancement by human bile of the binding of free and intrinsic factor-bound cobalamin (vitamin B12) to small bowel epithelial cell receptors.
Am J Gastroenterol. 1985 Dec; 80(12):964-9.AJ

Abstract

The effect of human bile on the binding of free cobalamin (Cbl) and of intrinsic factor-Cbl (IF-Cbl) complexes to intestine receptors was examined in in vitro receptor extracts made from guinea pig small bowel epithelial cells. Cbl and IF-Cbl complex binding to the receptors was measured in the effluent from a Sephadex G-200 column. Intrinsic factor-Cbl binding to the receptors in the control samples was 4.32 +/- 0.07 ng/animal (mean +/- SEM); addition of bile, saturated with excess 57Co-Cbl enhanced binding to 5.26 +/- 0.16 ng/animal (p less than 0.001). These data suggest that bile enhances binding of IF-Cbl complex onto ileal receptors. Whether bile also serves as "releasing factor" by releasing Cbl from intrinsic factor in vivo remains to be determined.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3000169

Citation

Kanazawa, S, et al. "Enhancement By Human Bile of the Binding of Free and Intrinsic Factor-bound Cobalamin (vitamin B12) to Small Bowel Epithelial Cell Receptors." The American Journal of Gastroenterology, vol. 80, no. 12, 1985, pp. 964-9.
Kanazawa S, Herzlich B, Herbert V. Enhancement by human bile of the binding of free and intrinsic factor-bound cobalamin (vitamin B12) to small bowel epithelial cell receptors. Am J Gastroenterol. 1985;80(12):964-9.
Kanazawa, S., Herzlich, B., & Herbert, V. (1985). Enhancement by human bile of the binding of free and intrinsic factor-bound cobalamin (vitamin B12) to small bowel epithelial cell receptors. The American Journal of Gastroenterology, 80(12), 964-9.
Kanazawa S, Herzlich B, Herbert V. Enhancement By Human Bile of the Binding of Free and Intrinsic Factor-bound Cobalamin (vitamin B12) to Small Bowel Epithelial Cell Receptors. Am J Gastroenterol. 1985;80(12):964-9. PubMed PMID: 3000169.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhancement by human bile of the binding of free and intrinsic factor-bound cobalamin (vitamin B12) to small bowel epithelial cell receptors. AU - Kanazawa,S, AU - Herzlich,B, AU - Herbert,V, PY - 1985/12/1/pubmed PY - 1985/12/1/medline PY - 1985/12/1/entrez SP - 964 EP - 9 JF - The American journal of gastroenterology JO - Am J Gastroenterol VL - 80 IS - 12 N2 - The effect of human bile on the binding of free cobalamin (Cbl) and of intrinsic factor-Cbl (IF-Cbl) complexes to intestine receptors was examined in in vitro receptor extracts made from guinea pig small bowel epithelial cells. Cbl and IF-Cbl complex binding to the receptors was measured in the effluent from a Sephadex G-200 column. Intrinsic factor-Cbl binding to the receptors in the control samples was 4.32 +/- 0.07 ng/animal (mean +/- SEM); addition of bile, saturated with excess 57Co-Cbl enhanced binding to 5.26 +/- 0.16 ng/animal (p less than 0.001). These data suggest that bile enhances binding of IF-Cbl complex onto ileal receptors. Whether bile also serves as "releasing factor" by releasing Cbl from intrinsic factor in vivo remains to be determined. SN - 0002-9270 UR - https://www.unboundmedicine.com/medline/citation/3000169/Enhancement_by_human_bile_of_the_binding_of_free_and_intrinsic_factor_bound_cobalamin__vitamin_B12__to_small_bowel_epithelial_cell_receptors_ DB - PRIME DP - Unbound Medicine ER -