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Terminalia bellirica (Gaertn.) Roxb fruit exerts anti-inflammatory effect via regulating arachidonic acid pathway and pro-inflammatory cytokines in lipopolysaccharide-induced RAW 264.7 macrophages.

Abstract

Terminalia bellirica (Gaertn.) Roxb. (Family: Combretaceae), known as Bhibhitaki in Sanskrit and locally known as Behera in India, has been used for centuries in Ayurveda, a universal system of medicine in India. The dried fruit of T. bellirica is used for the treatment of several disorders. The present study aims to explore the anti-inflammatory effects of aqueous acetone extracts isolated from T. bellirica (AATB) in RAW 264.7 cell lines. The AATB was prepared from the fruits of T. bellirica. Different concentrations of AATB (6.25-100 μg/ml) were used for MTT assay. The anti-inflammatory effect of AATB was evaluated by using different assays such as total cyclooxygenase (COX), 5-lipoxygenase (5-LOX) activity, nitrate and reactive oxygen species (ROS) production. The mRNA level expression of COX-2, tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) were studied in LPS stimulated RAW 264.7 cells. AATB treatment significantly diminished the elevated levels of inflammatory markers. Moreover, AATB downregulated the mRNA level expression of TNF-α, IL-6 and COX-2 genes. The result of our study suggest the use of AATB and is able to reduce inflammatory conditions associated with various diseases.

Authors+Show Affiliations

School of Biosciences, Mahatma Gandhi University, Priyadarshini Hills, Kottayam, Kerala, India.School of Biosciences, Mahatma Gandhi University, Priyadarshini Hills, Kottayam, Kerala, India.School of Biosciences, Mahatma Gandhi University, Priyadarshini Hills, Kottayam, Kerala, India.School of Biosciences, Mahatma Gandhi University, Priyadarshini Hills, Kottayam, Kerala, India.School of Biosciences, Mahatma Gandhi University, Priyadarshini Hills, Kottayam, Kerala, India. mslathasbs@yahoo.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30003465

Citation

Jayesh, Kuriakose, et al. "Terminalia Bellirica (Gaertn.) Roxb Fruit Exerts Anti-inflammatory Effect Via Regulating Arachidonic Acid Pathway and Pro-inflammatory Cytokines in Lipopolysaccharide-induced RAW 264.7 Macrophages." Inflammopharmacology, 2018.
Jayesh K, Karishma R, Vysakh A, et al. Terminalia bellirica (Gaertn.) Roxb fruit exerts anti-inflammatory effect via regulating arachidonic acid pathway and pro-inflammatory cytokines in lipopolysaccharide-induced RAW 264.7 macrophages. Inflammopharmacology. 2018.
Jayesh, K., Karishma, R., Vysakh, A., Gopika, P., & Latha, M. S. (2018). Terminalia bellirica (Gaertn.) Roxb fruit exerts anti-inflammatory effect via regulating arachidonic acid pathway and pro-inflammatory cytokines in lipopolysaccharide-induced RAW 264.7 macrophages. Inflammopharmacology, doi:10.1007/s10787-018-0513-x.
Jayesh K, et al. Terminalia Bellirica (Gaertn.) Roxb Fruit Exerts Anti-inflammatory Effect Via Regulating Arachidonic Acid Pathway and Pro-inflammatory Cytokines in Lipopolysaccharide-induced RAW 264.7 Macrophages. Inflammopharmacology. 2018 Jul 12; PubMed PMID: 30003465.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Terminalia bellirica (Gaertn.) Roxb fruit exerts anti-inflammatory effect via regulating arachidonic acid pathway and pro-inflammatory cytokines in lipopolysaccharide-induced RAW 264.7 macrophages. AU - Jayesh,Kuriakose, AU - Karishma,Raj, AU - Vysakh,A, AU - Gopika,Prasad, AU - Latha,M S, Y1 - 2018/07/12/ PY - 2018/04/25/received PY - 2018/07/03/accepted PY - 2018/7/14/entrez PY - 2018/7/14/pubmed PY - 2018/7/14/medline KW - Cytokines KW - Inflammation KW - Interleukin-6 KW - Terminalia bellirica JF - Inflammopharmacology JO - Inflammopharmacology N2 - Terminalia bellirica (Gaertn.) Roxb. (Family: Combretaceae), known as Bhibhitaki in Sanskrit and locally known as Behera in India, has been used for centuries in Ayurveda, a universal system of medicine in India. The dried fruit of T. bellirica is used for the treatment of several disorders. The present study aims to explore the anti-inflammatory effects of aqueous acetone extracts isolated from T. bellirica (AATB) in RAW 264.7 cell lines. The AATB was prepared from the fruits of T. bellirica. Different concentrations of AATB (6.25-100 μg/ml) were used for MTT assay. The anti-inflammatory effect of AATB was evaluated by using different assays such as total cyclooxygenase (COX), 5-lipoxygenase (5-LOX) activity, nitrate and reactive oxygen species (ROS) production. The mRNA level expression of COX-2, tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) were studied in LPS stimulated RAW 264.7 cells. AATB treatment significantly diminished the elevated levels of inflammatory markers. Moreover, AATB downregulated the mRNA level expression of TNF-α, IL-6 and COX-2 genes. The result of our study suggest the use of AATB and is able to reduce inflammatory conditions associated with various diseases. SN - 1568-5608 UR - https://www.unboundmedicine.com/medline/citation/30003465/Terminalia_bellirica_(Gaertn.)_Roxb_fruit_exerts_anti-inflammatory_effect_via_regulating_arachidonic_acid_pathway_and_pro-inflammatory_cytokines_in_lipopolysaccharide-induced_RAW_264.7_macrophages DB - PRIME DP - Unbound Medicine ER -