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Age-induced diminution of free radicals by Boeravinone B in Caenorhabditis elegans.
Exp Gerontol 2018; 111:94-106EG

Abstract

Oxidative damage is accrual of molecular deterioration from reactive oxygen species (ROS) while decrease in generation of ROS is related with free radical scavenging enzymes. Boerhaavia diffusa L. (Nyctaginaceae) derived novel molecule Boeravinone B (BOB) possesses a variety of pharmacological activities, yet their anti-aging potential has not been explored. The aim of the present study was to elucidate the mechanism of BOB mediated oxidative stress resistance and lifespan extension in Caenorhabditis elegans. The results showed that the BOB significantly extends the lifespan of C. elegans with its anti-oxidative potential via reducing accumulation of reactive oxygen species (ROS). BOB was found to recover the shortened lifespan of oxidative stress prone mutants mev-1 and gas-1 (14.75 and 16.11%, respectively). Additionally, this finding supported by the reduced ROS levels seen in BOB treated worms. Further, the effective concentration of BOB (25 μM) significantly enhanced the expressions of target genes such as superoxide dismutase (SOD-3), glutathione-S-transferase (GST-4) and heat shock protein (HSP-16.2) fused to green fluorescent protein (GFP), and it does so by modulating the stress-related signaling pathways (SEK-1) and transcription factors (SKN-1/Nrf and DAF-16/Foxo). Moreover, BOB exposure (25 μM) caused significant changes of age-dependent biomarkers such as pharyngeal pumping, body bend, locomotor activity and lipofuscin accumulation were also showed that BOB retards the aging. Overall, the findings highlight the antioxidant supplement triggering pharmaceutical potential of BOB which may serve as a new future perspective for healthy aging or delayed onset of oxidative related diseases.

Authors+Show Affiliations

Aging Biology Lab, Microbial Technology and Nematology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow 226 015, India; Academy of Scientific & Innovative Research (AcSIR), New Delhi, India.Aging Biology Lab, Microbial Technology and Nematology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow 226 015, India; Academy of Scientific & Innovative Research (AcSIR), New Delhi, India. Electronic address: r.pandey@cimap.res.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30004006

Citation

Rathor, Laxmi, and Rakesh Pandey. "Age-induced Diminution of Free Radicals By Boeravinone B in Caenorhabditis Elegans." Experimental Gerontology, vol. 111, 2018, pp. 94-106.
Rathor L, Pandey R. Age-induced diminution of free radicals by Boeravinone B in Caenorhabditis elegans. Exp Gerontol. 2018;111:94-106.
Rathor, L., & Pandey, R. (2018). Age-induced diminution of free radicals by Boeravinone B in Caenorhabditis elegans. Experimental Gerontology, 111, pp. 94-106. doi:10.1016/j.exger.2018.07.005.
Rathor L, Pandey R. Age-induced Diminution of Free Radicals By Boeravinone B in Caenorhabditis Elegans. Exp Gerontol. 2018 10 1;111:94-106. PubMed PMID: 30004006.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Age-induced diminution of free radicals by Boeravinone B in Caenorhabditis elegans. AU - Rathor,Laxmi, AU - Pandey,Rakesh, Y1 - 2018/07/10/ PY - 2017/07/12/received PY - 2018/06/27/revised PY - 2018/07/03/accepted PY - 2018/7/14/pubmed PY - 2018/7/14/medline PY - 2018/7/14/entrez KW - Age-related disorders KW - Boeravinone B KW - Caenorhabditis elegans KW - Longevity KW - Stress resistance SP - 94 EP - 106 JF - Experimental gerontology JO - Exp. Gerontol. VL - 111 N2 - Oxidative damage is accrual of molecular deterioration from reactive oxygen species (ROS) while decrease in generation of ROS is related with free radical scavenging enzymes. Boerhaavia diffusa L. (Nyctaginaceae) derived novel molecule Boeravinone B (BOB) possesses a variety of pharmacological activities, yet their anti-aging potential has not been explored. The aim of the present study was to elucidate the mechanism of BOB mediated oxidative stress resistance and lifespan extension in Caenorhabditis elegans. The results showed that the BOB significantly extends the lifespan of C. elegans with its anti-oxidative potential via reducing accumulation of reactive oxygen species (ROS). BOB was found to recover the shortened lifespan of oxidative stress prone mutants mev-1 and gas-1 (14.75 and 16.11%, respectively). Additionally, this finding supported by the reduced ROS levels seen in BOB treated worms. Further, the effective concentration of BOB (25 μM) significantly enhanced the expressions of target genes such as superoxide dismutase (SOD-3), glutathione-S-transferase (GST-4) and heat shock protein (HSP-16.2) fused to green fluorescent protein (GFP), and it does so by modulating the stress-related signaling pathways (SEK-1) and transcription factors (SKN-1/Nrf and DAF-16/Foxo). Moreover, BOB exposure (25 μM) caused significant changes of age-dependent biomarkers such as pharyngeal pumping, body bend, locomotor activity and lipofuscin accumulation were also showed that BOB retards the aging. Overall, the findings highlight the antioxidant supplement triggering pharmaceutical potential of BOB which may serve as a new future perspective for healthy aging or delayed onset of oxidative related diseases. SN - 1873-6815 UR - https://www.unboundmedicine.com/medline/citation/30004006/Age_induced_diminution_of_free_radicals_by_Boeravinone_B_in_Caenorhabditis_elegans_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0531-5565(17)30530-2 DB - PRIME DP - Unbound Medicine ER -