Tags

Type your tag names separated by a space and hit enter

Brown propolis-metabolomic innovative approach to determine compounds capable of killing Staphylococcus aureus biofilm and Trichomonas vaginalis.
Food Res Int 2018; 111:661-673FR

Abstract

Propolis, a resin produced by bees, is widely used in industrial products, including food, cosmetics, supplements, and pharmaceuticals. Extracts (ethanolic and hydroethanolic) and fractions, yielded by accelerated solvent extraction methodology, were obtained from different samples of Brazilian brown propolis (BBP). They were evaluated for antioxidant capacity, antibacterial, antibiofilm, and anti-Trichomonas vaginalis activities. The metabolomics profiling was determined by LC-DAD-MS and an innovative application of statistical analyses (univariate and chemometrics) was applied to correlate chemical compounds with biological activities. Eighty-six compounds were identified, including phenylpropanoic acids, flavonoids, chlorogenic acids, and prenylated phenylpropanoic acids. Propolis-fractions killed about 93% of Staphylococcus aureus in biofilm (at concentration of 125 μg/mL), showed activity against T. vaginalis with MIC at 400 μg/mL and significative antioxidant capacity (IC50 2.32-3.80 μg/mL). Propolis extracts and fractions did not show antibacterial and antibiofilm activities against Pseudomonas aeruginosa. The prenylated phenylpropanoic acids positively correlated with both the antibiofilm (S. aureus) and anti-T. vaginalis activities, such as the metabolites artepillin C, drupanin, and baccharin.

Authors+Show Affiliations

Laboratório de Produtos Naturais e Espectrometria de Massas (LaPNEM), Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição (FACFAN), Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS 79070-900, Brazil.Laboratório de Biofilmes e Diversidade Microbiana (LaBDiM), Faculdade de Farmácia e Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS 90040-060, Brazil; Departamento de Ciências Básicas da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, RS 90050-170, Brazil.Laboratório de Produtos Naturais e Espectrometria de Massas (LaPNEM), Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição (FACFAN), Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS 79070-900, Brazil.Laboratório de Pesquisa em Parasitologia, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.Laboratório de Biofilmes e Diversidade Microbiana (LaBDiM), Faculdade de Farmácia e Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS 90040-060, Brazil.Laboratório de Pesquisa em Parasitologia, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.Laboratório de Biofilmes e Diversidade Microbiana (LaBDiM), Faculdade de Farmácia e Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS 90040-060, Brazil.Laboratório de Produtos Naturais e Espectrometria de Massas (LaPNEM), Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição (FACFAN), Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS 79070-900, Brazil.Laboratório de Produtos Naturais e Espectrometria de Massas (LaPNEM), Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição (FACFAN), Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS 79070-900, Brazil. Electronic address: denise.brentan@ufms.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30007730

Citation

de Oliveira Dembogurski, Djaceli Sampaio, et al. "Brown Propolis-metabolomic Innovative Approach to Determine Compounds Capable of Killing Staphylococcus Aureus Biofilm and Trichomonas Vaginalis." Food Research International (Ottawa, Ont.), vol. 111, 2018, pp. 661-673.
de Oliveira Dembogurski DS, Silva Trentin D, Boaretto AG, et al. Brown propolis-metabolomic innovative approach to determine compounds capable of killing Staphylococcus aureus biofilm and Trichomonas vaginalis. Food Res Int. 2018;111:661-673.
de Oliveira Dembogurski, D. S., Silva Trentin, D., Boaretto, A. G., Rigo, G. V., da Silva, R. C., Tasca, T., ... Silva, D. B. (2018). Brown propolis-metabolomic innovative approach to determine compounds capable of killing Staphylococcus aureus biofilm and Trichomonas vaginalis. Food Research International (Ottawa, Ont.), 111, pp. 661-673. doi:10.1016/j.foodres.2018.05.033.
de Oliveira Dembogurski DS, et al. Brown Propolis-metabolomic Innovative Approach to Determine Compounds Capable of Killing Staphylococcus Aureus Biofilm and Trichomonas Vaginalis. Food Res Int. 2018;111:661-673. PubMed PMID: 30007730.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Brown propolis-metabolomic innovative approach to determine compounds capable of killing Staphylococcus aureus biofilm and Trichomonas vaginalis. AU - de Oliveira Dembogurski,Djaceli Sampaio, AU - Silva Trentin,Danielle, AU - Boaretto,Amanda Galdi, AU - Rigo,Graziela Vargas, AU - da Silva,Rodrigo Campos, AU - Tasca,Tiana, AU - Macedo,Alexandre José, AU - Carollo,Carlos Alexandre, AU - Silva,Denise Brentan, Y1 - 2018/05/23/ PY - 2018/02/25/received PY - 2018/05/06/revised PY - 2018/05/15/accepted PY - 2018/7/16/entrez PY - 2018/7/17/pubmed PY - 2019/10/16/medline KW - Antioxidant KW - Artepillin C KW - Drupanin KW - LC-DAD-MS KW - Prenylated phenylpropanoic acids KW - Univariate analyses SP - 661 EP - 673 JF - Food research international (Ottawa, Ont.) JO - Food Res. Int. VL - 111 N2 - Propolis, a resin produced by bees, is widely used in industrial products, including food, cosmetics, supplements, and pharmaceuticals. Extracts (ethanolic and hydroethanolic) and fractions, yielded by accelerated solvent extraction methodology, were obtained from different samples of Brazilian brown propolis (BBP). They were evaluated for antioxidant capacity, antibacterial, antibiofilm, and anti-Trichomonas vaginalis activities. The metabolomics profiling was determined by LC-DAD-MS and an innovative application of statistical analyses (univariate and chemometrics) was applied to correlate chemical compounds with biological activities. Eighty-six compounds were identified, including phenylpropanoic acids, flavonoids, chlorogenic acids, and prenylated phenylpropanoic acids. Propolis-fractions killed about 93% of Staphylococcus aureus in biofilm (at concentration of 125 μg/mL), showed activity against T. vaginalis with MIC at 400 μg/mL and significative antioxidant capacity (IC50 2.32-3.80 μg/mL). Propolis extracts and fractions did not show antibacterial and antibiofilm activities against Pseudomonas aeruginosa. The prenylated phenylpropanoic acids positively correlated with both the antibiofilm (S. aureus) and anti-T. vaginalis activities, such as the metabolites artepillin C, drupanin, and baccharin. SN - 1873-7145 UR - https://www.unboundmedicine.com/medline/citation/30007730/Brown_propolis_metabolomic_innovative_approach_to_determine_compounds_capable_of_killing_Staphylococcus_aureus_biofilm_and_Trichomonas_vaginalis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0963-9969(18)30386-7 DB - PRIME DP - Unbound Medicine ER -