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The Interaction between DELLA and ARF/IAA Mediates Crosstalk between Gibberellin and Auxin Signaling to Control Fruit Initiation in Tomato.
Plant Cell. 2018 08; 30(8):1710-1728.PC

Abstract

Fruit initiation following fertilization in angiosperms is strictly regulated by phytohormones. In tomato (Solanum lycopersicum), auxin and gibberellin (GA) play central roles in promoting fruit initiation. Without fertilization, elevated GA or auxin signaling can induce parthenocarpy (seedless fruit production). The GA-signaling repressor SlDELLA and auxin-signaling components SlIAA9 and SlARF7 repress parthenocarpy, but the underlying mechanism is unknown. Here, we show that SlDELLA and the SlARF7/SlIAA9 complex mediate crosstalk between GA and auxin pathways to regulate fruit initiation. Yeast-two-hybrid and coimmunoprecipitation assays showed that SlARF7 and additional activator SlARFs interact with SlDELLA and SlIAA9 through distinct domains. SlARF7/SlIAA9 and SlDELLA antagonistically modulate the expression of feedback-regulated genes involved in GA and auxin metabolism, whereas SlARF7/SlIAA9 and SlDELLA coregulate the expression of fruit growth-related genes. Analysis of procera (della), SlARF7 RNAi (with downregulated expression of multiple activator SlARFs), and entire (iaa9) single and double mutants indicated that these genes additively affect parthenocarpy, supporting the notion that the SlARFs/SlIAA9 and SlDELLA interaction plays an important role in regulating fruit initiation. Analysis of the GA-deficient mutant gib1 showed that active GA biosynthesis and signaling are required for auxin-induced fruit initiation. Our study reveals how direct crosstalk between auxin- and GA-signaling components is critical for tomato fruit initiation.

Authors+Show Affiliations

Department of Biology, Duke University, Durham, North Carolina 27708.The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Hebrew University, Rehovot 76100, Israel.The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Hebrew University, Rehovot 76100, Israel.Department of Biology, Duke University, Durham, North Carolina 27708 tps@duke.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

30008445

Citation

Hu, Jianhong, et al. "The Interaction Between DELLA and ARF/IAA Mediates Crosstalk Between Gibberellin and Auxin Signaling to Control Fruit Initiation in Tomato." The Plant Cell, vol. 30, no. 8, 2018, pp. 1710-1728.
Hu J, Israeli A, Ori N, et al. The Interaction between DELLA and ARF/IAA Mediates Crosstalk between Gibberellin and Auxin Signaling to Control Fruit Initiation in Tomato. Plant Cell. 2018;30(8):1710-1728.
Hu, J., Israeli, A., Ori, N., & Sun, T. P. (2018). The Interaction between DELLA and ARF/IAA Mediates Crosstalk between Gibberellin and Auxin Signaling to Control Fruit Initiation in Tomato. The Plant Cell, 30(8), 1710-1728. https://doi.org/10.1105/tpc.18.00363
Hu J, et al. The Interaction Between DELLA and ARF/IAA Mediates Crosstalk Between Gibberellin and Auxin Signaling to Control Fruit Initiation in Tomato. Plant Cell. 2018;30(8):1710-1728. PubMed PMID: 30008445.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Interaction between DELLA and ARF/IAA Mediates Crosstalk between Gibberellin and Auxin Signaling to Control Fruit Initiation in Tomato. AU - Hu,Jianhong, AU - Israeli,Alon, AU - Ori,Naomi, AU - Sun,Tai-Ping, Y1 - 2018/07/15/ PY - 2018/05/07/received PY - 2018/06/21/revised PY - 2018/07/12/accepted PY - 2018/7/17/pubmed PY - 2019/6/22/medline PY - 2018/7/17/entrez SP - 1710 EP - 1728 JF - The Plant cell JO - Plant Cell VL - 30 IS - 8 N2 - Fruit initiation following fertilization in angiosperms is strictly regulated by phytohormones. In tomato (Solanum lycopersicum), auxin and gibberellin (GA) play central roles in promoting fruit initiation. Without fertilization, elevated GA or auxin signaling can induce parthenocarpy (seedless fruit production). The GA-signaling repressor SlDELLA and auxin-signaling components SlIAA9 and SlARF7 repress parthenocarpy, but the underlying mechanism is unknown. Here, we show that SlDELLA and the SlARF7/SlIAA9 complex mediate crosstalk between GA and auxin pathways to regulate fruit initiation. Yeast-two-hybrid and coimmunoprecipitation assays showed that SlARF7 and additional activator SlARFs interact with SlDELLA and SlIAA9 through distinct domains. SlARF7/SlIAA9 and SlDELLA antagonistically modulate the expression of feedback-regulated genes involved in GA and auxin metabolism, whereas SlARF7/SlIAA9 and SlDELLA coregulate the expression of fruit growth-related genes. Analysis of procera (della), SlARF7 RNAi (with downregulated expression of multiple activator SlARFs), and entire (iaa9) single and double mutants indicated that these genes additively affect parthenocarpy, supporting the notion that the SlARFs/SlIAA9 and SlDELLA interaction plays an important role in regulating fruit initiation. Analysis of the GA-deficient mutant gib1 showed that active GA biosynthesis and signaling are required for auxin-induced fruit initiation. Our study reveals how direct crosstalk between auxin- and GA-signaling components is critical for tomato fruit initiation. SN - 1532-298X UR - https://www.unboundmedicine.com/medline/citation/30008445/The_Interaction_between_DELLA_and_ARF/IAA_Mediates_Crosstalk_between_Gibberellin_and_Auxin_Signaling_to_Control_Fruit_Initiation_in_Tomato_ L2 - http://www.plantcell.org/cgi/pmidlookup?view=long&pmid=30008445 DB - PRIME DP - Unbound Medicine ER -