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Phytochemical analysis and antimicrobial activity of some medicinal plants against selected pathogenic microorganisms.
Microb Pathog. 2018 Oct; 123:219-226.MP

Abstract

The aim of the present study is to investigate the antimicrobial potency of leaves from various extracts of Capparis zeylanica, Streblus asper and Tribulus terrestris were evaluated. In addition, this is the first report on MIC, MBC/MFC antimicrobial activities of above mentioned plants and also identify the phytochemical, functional groups by GC-MS and FT-IR respectively. Soxhlet extraction method was used for preparation of different extracts viz., aqueous, petroleum ether, ethyl acetate and methanol. The extracts were examined against Staphylococcus epidermidis, Enterococcus faecallis, Salmonella paratyphi, Shigella dysenteriae, Candida albicans and Mycobacterium tuberculosis by agar well diffusion method, and Minimum Inhibitory Concentratioon (MIC), Minimum Bactericidal/Fungicidal Concentration (MBC/MFC) values were determined through micro dilution method. Phytochemical analysis of compounds was carried out by GC-MS analysis and functional groups were identified by FT-IR. Based on the outcome of our results, Ethyl acetate extract Showed significant antimicrobial activity against the tested pathogens especially, for C. albicans (40 mm) followed by ethyl acetate of S. asper against S. paratyphi (38 mm). While, the least inhibition was observed with aqueous extract of T. terrestris against S. paratyphi (10 mm). The MIC ranged from 3.21 mg/ml to 50 mg/ml and MBC/MFC 6.25 mg/ml to 50 mg/ml was recorded. Ethyl acetate extracts of almost all samples showed better activity than other extracts in inhibition growth of pathogens. Phytochemical analysis exhibited the presence of Steroids, tannins and cardiac glycosides were found only in ethyl acetate extract of C. zeylanica. Functional group of leaf extract was confirmed by FT-IR spectrum and GC-MS analysis of the ethyl acetate extract revealed the presence of 20 compounds. The results revealed that ethyl acetate extract of C. zeylanica leaves has potential activity than the other extracts as well as standard drugs (Gentamycin and Ketocozole). Hence, this plant may be recommended for further studies in isolation of active compounds and related pharmacological activities.

Authors+Show Affiliations

Computational Phytochemistry Lab, PG and Research Department of Botany and Microbiology, AVVM Sri Pushpam College (Autonomous) Poondi, Thanjavur (Dist), Tamil Nadu, India.Computational Phytochemistry Lab, PG and Research Department of Botany and Microbiology, AVVM Sri Pushpam College (Autonomous) Poondi, Thanjavur (Dist), Tamil Nadu, India. Electronic address: svijaya_kumar2579@rediff.com.Computational Phytochemistry Lab, PG and Research Department of Botany and Microbiology, AVVM Sri Pushpam College (Autonomous) Poondi, Thanjavur (Dist), Tamil Nadu, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30009969

Citation

Arulmozhi, P, et al. "Phytochemical Analysis and Antimicrobial Activity of some Medicinal Plants Against Selected Pathogenic Microorganisms." Microbial Pathogenesis, vol. 123, 2018, pp. 219-226.
Arulmozhi P, Vijayakumar S, Kumar T. Phytochemical analysis and antimicrobial activity of some medicinal plants against selected pathogenic microorganisms. Microb Pathog. 2018;123:219-226.
Arulmozhi, P., Vijayakumar, S., & Kumar, T. (2018). Phytochemical analysis and antimicrobial activity of some medicinal plants against selected pathogenic microorganisms. Microbial Pathogenesis, 123, 219-226. https://doi.org/10.1016/j.micpath.2018.07.009
Arulmozhi P, Vijayakumar S, Kumar T. Phytochemical Analysis and Antimicrobial Activity of some Medicinal Plants Against Selected Pathogenic Microorganisms. Microb Pathog. 2018;123:219-226. PubMed PMID: 30009969.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phytochemical analysis and antimicrobial activity of some medicinal plants against selected pathogenic microorganisms. AU - Arulmozhi,P, AU - Vijayakumar,S, AU - Kumar,T, Y1 - 2018/07/19/ PY - 2018/01/09/received PY - 2018/07/10/revised PY - 2018/07/11/accepted PY - 2018/7/17/pubmed PY - 2018/12/12/medline PY - 2018/7/17/entrez KW - Antimicrobial activity KW - Capparis zeylanica KW - FTIR KW - GC-MS KW - MBC/MFC KW - MIC KW - Phytochemicals SP - 219 EP - 226 JF - Microbial pathogenesis JO - Microb Pathog VL - 123 N2 - The aim of the present study is to investigate the antimicrobial potency of leaves from various extracts of Capparis zeylanica, Streblus asper and Tribulus terrestris were evaluated. In addition, this is the first report on MIC, MBC/MFC antimicrobial activities of above mentioned plants and also identify the phytochemical, functional groups by GC-MS and FT-IR respectively. Soxhlet extraction method was used for preparation of different extracts viz., aqueous, petroleum ether, ethyl acetate and methanol. The extracts were examined against Staphylococcus epidermidis, Enterococcus faecallis, Salmonella paratyphi, Shigella dysenteriae, Candida albicans and Mycobacterium tuberculosis by agar well diffusion method, and Minimum Inhibitory Concentratioon (MIC), Minimum Bactericidal/Fungicidal Concentration (MBC/MFC) values were determined through micro dilution method. Phytochemical analysis of compounds was carried out by GC-MS analysis and functional groups were identified by FT-IR. Based on the outcome of our results, Ethyl acetate extract Showed significant antimicrobial activity against the tested pathogens especially, for C. albicans (40 mm) followed by ethyl acetate of S. asper against S. paratyphi (38 mm). While, the least inhibition was observed with aqueous extract of T. terrestris against S. paratyphi (10 mm). The MIC ranged from 3.21 mg/ml to 50 mg/ml and MBC/MFC 6.25 mg/ml to 50 mg/ml was recorded. Ethyl acetate extracts of almost all samples showed better activity than other extracts in inhibition growth of pathogens. Phytochemical analysis exhibited the presence of Steroids, tannins and cardiac glycosides were found only in ethyl acetate extract of C. zeylanica. Functional group of leaf extract was confirmed by FT-IR spectrum and GC-MS analysis of the ethyl acetate extract revealed the presence of 20 compounds. The results revealed that ethyl acetate extract of C. zeylanica leaves has potential activity than the other extracts as well as standard drugs (Gentamycin and Ketocozole). Hence, this plant may be recommended for further studies in isolation of active compounds and related pharmacological activities. SN - 1096-1208 UR - https://www.unboundmedicine.com/medline/citation/30009969/Phytochemical_analysis_and_antimicrobial_activity_of_some_medicinal_plants_against_selected_pathogenic_microorganisms_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0882-4010(18)30050-0 DB - PRIME DP - Unbound Medicine ER -