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Sargassum serratifolium attenuates RANKL-induced osteoclast differentiation and oxidative stress through inhibition of NF-κB and activation of the Nrf2/HO-1 signaling pathway.
Biosci Trends. 2018; 12(3):257-265.BT

Abstract

Sargassum serratifolium C. Agardh is a marine brown alga that has long been used as an ingredient for food and medicine by many people living along Asian coastlines. Recently, various beneficial effects of extracts or compounds isolated from S. serratifolium have been reported, but their efficacies against bone destruction are unclear. Therefore, in this study, we investigated the inhibitory property of an ethanol extract of S. serratifolium (EESS) on osteoclast differentiation by focusing on the receptor activator of nuclear factor-κB ligand (RANKL)-stimulated osteoclastogenesis model using RAW 264.7 macrophages. Our results demonstrated that EESS reduced RANKL-induced osteoclast differentiation in RAW 264.7 cells, by inhibiting tartrate-resistant acid phosphatase (TRAP) activity and destroying the F-actin ring formation. EESS also attenuated RANKL-induced expressions of key osteoclast-specific genes, such as nuclear factor of activated T cells cytoplasmic 1 (NFATC1), TRAP, cathepsin K and matrix metalloproteinase-9. These effects were mediated by impaired nuclear translocation of nuclear factor (NF)-κB and suppression of IκB-α degradation. In addition, EESS effectively inhibited the production of reactive oxygen species (ROS) by RANKL, which was associated with enhanced expression of nuclear translocation of nuclear factor-erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1). Overall, our findings provide evidence that EESS suppresses RANKL-induced osteoclastogenesis and oxidative stress through suppression of NF-κB and activation of Nrf2/HO-1 signaling pathway, indicating that S. serratifolium has a potential application the prevention and treatment of osteoclastogenic bone disease.

Authors+Show Affiliations

Anti-Aging Research Center, Dongeui University.Department of Molecular Biology, College of Natural Sciences, Dongeui University.Department of Marine Life Sciences, Jeju National University.Department of Oral Pathology and Regenerative Medicine, School of Dentistry, Institute for Hard Tissue and Biotooth Regeneration, Kyungpook National University.Department of Marine Life Sciences, Jeju National University.Department of Chemistry, College of Natural Sciences, Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University.Department of Food and Nutrition, College of Natural Sciences and Human Ecology, Dongeui University.Anti-Aging Research Center, Dongeui University. Department of Biochemistry, Dongeui University College of Korean Medicine.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30012915

Citation

Kim, Hong Jae, et al. "Sargassum Serratifolium Attenuates RANKL-induced Osteoclast Differentiation and Oxidative Stress Through Inhibition of NF-κB and Activation of the Nrf2/HO-1 Signaling Pathway." Bioscience Trends, vol. 12, no. 3, 2018, pp. 257-265.
Kim HJ, Park C, Kim GY, et al. Sargassum serratifolium attenuates RANKL-induced osteoclast differentiation and oxidative stress through inhibition of NF-κB and activation of the Nrf2/HO-1 signaling pathway. Biosci Trends. 2018;12(3):257-265.
Kim, H. J., Park, C., Kim, G. Y., Park, E. K., Jeon, Y. J., Kim, S., Hwang, H. J., & Choi, Y. H. (2018). Sargassum serratifolium attenuates RANKL-induced osteoclast differentiation and oxidative stress through inhibition of NF-κB and activation of the Nrf2/HO-1 signaling pathway. Bioscience Trends, 12(3), 257-265. https://doi.org/10.5582/bst.2018.01107
Kim HJ, et al. Sargassum Serratifolium Attenuates RANKL-induced Osteoclast Differentiation and Oxidative Stress Through Inhibition of NF-κB and Activation of the Nrf2/HO-1 Signaling Pathway. Biosci Trends. 2018;12(3):257-265. PubMed PMID: 30012915.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sargassum serratifolium attenuates RANKL-induced osteoclast differentiation and oxidative stress through inhibition of NF-κB and activation of the Nrf2/HO-1 signaling pathway. AU - Kim,Hong Jae, AU - Park,Cheol, AU - Kim,Gi-Young, AU - Park,Eui Kyun, AU - Jeon,You-Jin, AU - Kim,Suhkmann, AU - Hwang,Hye Jin, AU - Choi,Yung Hyun, PY - 2018/7/18/entrez PY - 2018/7/18/pubmed PY - 2018/10/30/medline KW - NF-κB KW - Nrf2/HO-1 KW - RANKL KW - Sargassum serratifolium KW - osteoclasts SP - 257 EP - 265 JF - Bioscience trends JO - Biosci Trends VL - 12 IS - 3 N2 - Sargassum serratifolium C. Agardh is a marine brown alga that has long been used as an ingredient for food and medicine by many people living along Asian coastlines. Recently, various beneficial effects of extracts or compounds isolated from S. serratifolium have been reported, but their efficacies against bone destruction are unclear. Therefore, in this study, we investigated the inhibitory property of an ethanol extract of S. serratifolium (EESS) on osteoclast differentiation by focusing on the receptor activator of nuclear factor-κB ligand (RANKL)-stimulated osteoclastogenesis model using RAW 264.7 macrophages. Our results demonstrated that EESS reduced RANKL-induced osteoclast differentiation in RAW 264.7 cells, by inhibiting tartrate-resistant acid phosphatase (TRAP) activity and destroying the F-actin ring formation. EESS also attenuated RANKL-induced expressions of key osteoclast-specific genes, such as nuclear factor of activated T cells cytoplasmic 1 (NFATC1), TRAP, cathepsin K and matrix metalloproteinase-9. These effects were mediated by impaired nuclear translocation of nuclear factor (NF)-κB and suppression of IκB-α degradation. In addition, EESS effectively inhibited the production of reactive oxygen species (ROS) by RANKL, which was associated with enhanced expression of nuclear translocation of nuclear factor-erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1). Overall, our findings provide evidence that EESS suppresses RANKL-induced osteoclastogenesis and oxidative stress through suppression of NF-κB and activation of Nrf2/HO-1 signaling pathway, indicating that S. serratifolium has a potential application the prevention and treatment of osteoclastogenic bone disease. SN - 1881-7823 UR - https://www.unboundmedicine.com/medline/citation/30012915/Sargassum_serratifolium_attenuates_RANKL_induced_osteoclast_differentiation_and_oxidative_stress_through_inhibition_of_NF_κB_and_activation_of_the_Nrf2/HO_1_signaling_pathway_ L2 - https://dx.doi.org/10.5582/bst.2018.01107 DB - PRIME DP - Unbound Medicine ER -