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[Time-effect relationship and molecular mechanism of electroacupuncture for repair of gastric mucosal lesion].
Zhongguo Zhen Jiu. 2018 Jul 12; 38(7):747-52.ZZ

Abstract

OBJECTIVE

To dynamically observe the effects of electroacupuncture (EA) on repair of gastric mucosal lesion in rats with gastric ulcer, and to explore the time-effect relationship and molecular mechanism of EA for gastric ulcer.

METHODS

A total of 72 SD rats were randomly assigned to a normal group, a model group, a acupoint group and a sham acupoint group, and each group were further divided into a 1-day subgroup, a 4-day subgroup and a 7-day subgroup, 6 rats in each subgroup. The rat model of gastric ulcer was established by using intragastric administration of ethyl alcohol. The rats in the acupoint group were treated with EA at"Zusanli"(ST 36) and"Liangmen"(ST 21); the rats in the sham acupoint group were treated with EA at points 5 mm next to"Zusanli"(ST 36) and"Liangmen"(ST 21); the EA was given 30 min per treatment, once a day. The rats in the normal group and model group were treated with immobilization for 30 min per day, and no EA was given. PR-PCR method was applied to test the expression of proliferating cell nuclear antigen (PCNA) and substance P (SP); Western blot method was applied to test the expression of neurotensin (NT).

RESULTS

After 1-day treatment, the ulcer index in the model group was higher than that in the normal group (P<0.01), and the expression of PCNA, SP and NT was decreased (P<0.01, P<0.05); compared with the model group and sham acupoint group, the ulcer index was decreased in the acupoint group (both P<0.05), and the expression of PCNA and SP was up-regulated (all P<0.05) while that of NT was up-regulated (both P<0.01). After 4-day treatment, the ulcer index in the model group was reduced but still higher than that in the normal group (P<0.05), and the expression of PCNA, SP and NT was up-regulated and higher than that in the normal group (all P<0.01); the ulcer index in the acupoint group was similar to that in the normal group (P>0.05), and the expression of PCNA and SP was lower than that in the model group (P<0.01, P<0.05), and the expression of NT was not significantly different from that in the model group (P>0.05). After 7-day treatment, the differences of indexes above were not significant among the four groups (all P>0.05).

CONCLUSION

EA at acupoints of stomach meridian has two-way regulation on PCNA and SP and improve the expression of NT in different pathological state of gastric ulcer, which could further improve the repair of gastric ulcer.

Authors+Show Affiliations

Research Institute of Shenzhen, Medical College of Xiamen University, Shenzhen 518000, Guangdong Province, China; College of Acupuncture-Moxibustion and Tuina, Hunan University of CM, Changsha 410208; 3Fujian University of TCM.Research Institute of Shenzhen, Medical College of Xiamen University, Shenzhen 518000, Guangdong Province, China.Fujian University of TCM.Research Institute of Shenzhen, Medical College of Xiamen University, Shenzhen 518000, Guangdong Province, China.Fujian University of TCM.Fujian University of TCM.Research Institute of Shenzhen, Medical College of Xiamen University, Shenzhen 518000, Guangdong Province, China.Research Institute of Shenzhen, Medical College of Xiamen University, Shenzhen 518000, Guangdong Province, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

30014670

Citation

Zhang, Yuan, et al. "[Time-effect Relationship and Molecular Mechanism of Electroacupuncture for Repair of Gastric Mucosal Lesion]." Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, vol. 38, no. 7, 2018, pp. 747-52.
Zhang Y, Liu C, Lian L, et al. [Time-effect relationship and molecular mechanism of electroacupuncture for repair of gastric mucosal lesion]. Zhongguo Zhen Jiu. 2018;38(7):747-52.
Zhang, Y., Liu, C., Lian, L., Shen, J., He, Q., Chen, J., Ma, F., & Yang, Z. (2018). [Time-effect relationship and molecular mechanism of electroacupuncture for repair of gastric mucosal lesion]. Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, 38(7), 747-52. https://doi.org/10.13703/j.0255-2930.2018.07.018
Zhang Y, et al. [Time-effect Relationship and Molecular Mechanism of Electroacupuncture for Repair of Gastric Mucosal Lesion]. Zhongguo Zhen Jiu. 2018 Jul 12;38(7):747-52. PubMed PMID: 30014670.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Time-effect relationship and molecular mechanism of electroacupuncture for repair of gastric mucosal lesion]. AU - Zhang,Yuan, AU - Liu,Caichun, AU - Lian,Linyu, AU - Shen,Jiacheng, AU - He,Qida, AU - Chen,Jiaolong, AU - Ma,Fuqiang, AU - Yang,Zongbao, PY - 2018/7/18/entrez PY - 2018/7/18/pubmed PY - 2019/6/1/medline KW - Point ST 21 (Liangmen) KW - Point ST 36 (Zusanli) KW - electroacupuncture (EA) KW - gastric ulcer KW - neurotensin(NT) KW - proliferating cell nuclear antigen (PCNA) KW - substance P (SP) KW - time-effect relationship SP - 747 EP - 52 JF - Zhongguo zhen jiu = Chinese acupuncture & moxibustion JO - Zhongguo Zhen Jiu VL - 38 IS - 7 N2 - OBJECTIVE: To dynamically observe the effects of electroacupuncture (EA) on repair of gastric mucosal lesion in rats with gastric ulcer, and to explore the time-effect relationship and molecular mechanism of EA for gastric ulcer. METHODS: A total of 72 SD rats were randomly assigned to a normal group, a model group, a acupoint group and a sham acupoint group, and each group were further divided into a 1-day subgroup, a 4-day subgroup and a 7-day subgroup, 6 rats in each subgroup. The rat model of gastric ulcer was established by using intragastric administration of ethyl alcohol. The rats in the acupoint group were treated with EA at"Zusanli"(ST 36) and"Liangmen"(ST 21); the rats in the sham acupoint group were treated with EA at points 5 mm next to"Zusanli"(ST 36) and"Liangmen"(ST 21); the EA was given 30 min per treatment, once a day. The rats in the normal group and model group were treated with immobilization for 30 min per day, and no EA was given. PR-PCR method was applied to test the expression of proliferating cell nuclear antigen (PCNA) and substance P (SP); Western blot method was applied to test the expression of neurotensin (NT). RESULTS: After 1-day treatment, the ulcer index in the model group was higher than that in the normal group (P<0.01), and the expression of PCNA, SP and NT was decreased (P<0.01, P<0.05); compared with the model group and sham acupoint group, the ulcer index was decreased in the acupoint group (both P<0.05), and the expression of PCNA and SP was up-regulated (all P<0.05) while that of NT was up-regulated (both P<0.01). After 4-day treatment, the ulcer index in the model group was reduced but still higher than that in the normal group (P<0.05), and the expression of PCNA, SP and NT was up-regulated and higher than that in the normal group (all P<0.01); the ulcer index in the acupoint group was similar to that in the normal group (P>0.05), and the expression of PCNA and SP was lower than that in the model group (P<0.01, P<0.05), and the expression of NT was not significantly different from that in the model group (P>0.05). After 7-day treatment, the differences of indexes above were not significant among the four groups (all P>0.05). CONCLUSION: EA at acupoints of stomach meridian has two-way regulation on PCNA and SP and improve the expression of NT in different pathological state of gastric ulcer, which could further improve the repair of gastric ulcer. SN - 0255-2930 UR - https://www.unboundmedicine.com/medline/citation/30014670/[Time_effect_relationship_and_molecular_mechanism_of_electroacupuncture_for_repair_of_gastric_mucosal_lesion]_ L2 - https://antibodies.cancer.gov/detail/CPTC-PGR-2 DB - PRIME DP - Unbound Medicine ER -