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Comparison of Phosphatidylserine-Exposing Red Blood Cells, Fragmented Red Blood Cells and Red Blood Cell-Derived Microparticles in β-Thalassemia/HbE Patients.
Lab Med 2019; 50(1):47-53LM

Abstract

Objective

To determine the number and intensity of phosphatidylserine (PS) expression of the red blood cells (RBCs), fragmented RBCs, and RBC-derived microparticles (RMPs) in patients with β-thalassemia/hemoglobin (Hb)E.

Methods

We used flow cytometry to determine the number and levels of PS expression.

Results

The number of PS-exposing RBCs was statistically significantly higher (P <.001) than that of PS-exposing fragmented RBCs or RMPs. In contrast, the intensity of PS expression was significantly higher (P <.001) in RMPs than in RBCs or fragmented RBCs. Our study showed a trend of association between RBC distribution width (RDW) and both the number of fragmented RBCs and RMPs and their intensity of PS expression.

Conclusion

In β-thalassemia/HbE, PS-exposing RBCs, fragmented RBCs, and RMPs all differed in their numbers and their intensity of PS expression. The effects of these differences among PS-exposing populations on the pathophysiology of the disease require further investigation.

Authors+Show Affiliations

Research Division, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.Department of Pediatrics, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30016461

Citation

Noulsri, Egarit, et al. "Comparison of Phosphatidylserine-Exposing Red Blood Cells, Fragmented Red Blood Cells and Red Blood Cell-Derived Microparticles in β-Thalassemia/HbE Patients." Laboratory Medicine, vol. 50, no. 1, 2019, pp. 47-53.
Noulsri E, Ardsiri S, Lerdwana S, et al. Comparison of Phosphatidylserine-Exposing Red Blood Cells, Fragmented Red Blood Cells and Red Blood Cell-Derived Microparticles in β-Thalassemia/HbE Patients. Lab Med. 2019;50(1):47-53.
Noulsri, E., Ardsiri, S., Lerdwana, S., & Pattanapanyasat, K. (2019). Comparison of Phosphatidylserine-Exposing Red Blood Cells, Fragmented Red Blood Cells and Red Blood Cell-Derived Microparticles in β-Thalassemia/HbE Patients. Laboratory Medicine, 50(1), pp. 47-53. doi:10.1093/labmed/lmy039.
Noulsri E, et al. Comparison of Phosphatidylserine-Exposing Red Blood Cells, Fragmented Red Blood Cells and Red Blood Cell-Derived Microparticles in β-Thalassemia/HbE Patients. Lab Med. 2019 Jan 1;50(1):47-53. PubMed PMID: 30016461.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of Phosphatidylserine-Exposing Red Blood Cells, Fragmented Red Blood Cells and Red Blood Cell-Derived Microparticles in β-Thalassemia/HbE Patients. AU - Noulsri,Egarit, AU - Ardsiri,Sakkarin, AU - Lerdwana,Surada, AU - Pattanapanyasat,Kovit, PY - 2018/7/18/pubmed PY - 2019/1/15/medline PY - 2018/7/18/entrez SP - 47 EP - 53 JF - Laboratory medicine JO - Lab Med VL - 50 IS - 1 N2 - Objective: To determine the number and intensity of phosphatidylserine (PS) expression of the red blood cells (RBCs), fragmented RBCs, and RBC-derived microparticles (RMPs) in patients with β-thalassemia/hemoglobin (Hb)E. Methods: We used flow cytometry to determine the number and levels of PS expression. Results: The number of PS-exposing RBCs was statistically significantly higher (P <.001) than that of PS-exposing fragmented RBCs or RMPs. In contrast, the intensity of PS expression was significantly higher (P <.001) in RMPs than in RBCs or fragmented RBCs. Our study showed a trend of association between RBC distribution width (RDW) and both the number of fragmented RBCs and RMPs and their intensity of PS expression. Conclusion: In β-thalassemia/HbE, PS-exposing RBCs, fragmented RBCs, and RMPs all differed in their numbers and their intensity of PS expression. The effects of these differences among PS-exposing populations on the pathophysiology of the disease require further investigation. SN - 1943-7730 UR - https://www.unboundmedicine.com/medline/citation/30016461/Comparison_of_Phosphatidylserine-Exposing_Red_Blood_Cells,_Fragmented_Red_Blood_Cells_and_Red_Blood_Cell-Derived_Microparticles_in_β-Thalassemia/HbE_Patients L2 - https://academic.oup.com/labmed/article-lookup/doi/10.1093/labmed/lmy039 DB - PRIME DP - Unbound Medicine ER -