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Asiatic acid protects differentiated PC12 cells from Aβ25-35-induced apoptosis and tau hyperphosphorylation via regulating PI3K/Akt/GSK-3β signaling.
Life Sci. 2018 Sep 01; 208:96-101.LS

Abstract

Amyloid β (Aβ) peptide can cause neurotoxicity in Alzheimer's disease (AD). The main purpose of the present study is to investigate the protective role of asiatic acid (AA) against Aβ25-35-induced neurotoxicity in neuronally differentiated PC12 cells. Differentiated PC12 cells were pretreated with 5, 10 or 20 μM AA before treatment with 20 μM Aβ25-35. The viability and apoptosis of differentiated PC12 cells were determined by MTT assay and Annexin V-FITC/PI double staining, respectively. The mitochondrial membrane potential (MMP) of differentiated PC12 cells was analyzed by JC-1 staining. The expression levels of proteins were detected by western blot analysis. We found that AA significantly increased the viability of differentiated PC12 cells but attenuated the mitochondria-mediated apoptosis dose-dependently when challenging with Aβ25-35. Besides, the results of western blot analysis showed that AA prevented IκBα degradation and p65 nuclear translocation, and promoted the phosphorylation of Akt and GSK-3β in Aβ25-35-treated differentiated PC12 cells. Moreover, LY294002, a specific PI3K inhibitor, was found to abolish the beneficial effects of AA on Aβ25-35-induced apoptosis and tau protein hyperphosphorylation. Our findings demonstrated that AA protects differentiated PC12 cells from Aβ25-35-induced apoptosis and tau protein hyperphosphorylation, which might be partially mediated by the activation of the PI3K/Akt/GSK-3β signaling pathway.

Authors+Show Affiliations

Department of Neurology, No.1 People's Hospital of Jining City, Jining, Shandong, China.Department of Neurology, No.1 People's Hospital of Jining City, Jining, Shandong, China.Department of Neurology, No.1 People's Hospital of Jining City, Jining, Shandong, China.Department of Neurology, No.1 People's Hospital of Jining City, Jining, Shandong, China. Electronic address: chujf700214@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30017668

Citation

Cheng, Wenjing, et al. "Asiatic Acid Protects Differentiated PC12 Cells From Aβ25-35-induced Apoptosis and Tau Hyperphosphorylation Via Regulating PI3K/Akt/GSK-3β Signaling." Life Sciences, vol. 208, 2018, pp. 96-101.
Cheng W, Chen W, Wang P, et al. Asiatic acid protects differentiated PC12 cells from Aβ25-35-induced apoptosis and tau hyperphosphorylation via regulating PI3K/Akt/GSK-3β signaling. Life Sci. 2018;208:96-101.
Cheng, W., Chen, W., Wang, P., & Chu, J. (2018). Asiatic acid protects differentiated PC12 cells from Aβ25-35-induced apoptosis and tau hyperphosphorylation via regulating PI3K/Akt/GSK-3β signaling. Life Sciences, 208, 96-101. https://doi.org/10.1016/j.lfs.2018.07.016
Cheng W, et al. Asiatic Acid Protects Differentiated PC12 Cells From Aβ25-35-induced Apoptosis and Tau Hyperphosphorylation Via Regulating PI3K/Akt/GSK-3β Signaling. Life Sci. 2018 Sep 1;208:96-101. PubMed PMID: 30017668.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Asiatic acid protects differentiated PC12 cells from Aβ25-35-induced apoptosis and tau hyperphosphorylation via regulating PI3K/Akt/GSK-3β signaling. AU - Cheng,Wenjing, AU - Chen,Weimei, AU - Wang,Peng, AU - Chu,Jianfeng, Y1 - 2018/07/11/ PY - 2018/05/10/received PY - 2018/07/05/revised PY - 2018/07/10/accepted PY - 2018/7/19/pubmed PY - 2018/9/5/medline PY - 2018/7/19/entrez KW - Alzheimer's disease (AD) KW - Asiatic acid KW - Aβ(25–35) KW - NF-κB KW - PI3K/Akt/GSK-3β signaling KW - Tau hyperphosphorylation SP - 96 EP - 101 JF - Life sciences JO - Life Sci VL - 208 N2 - Amyloid β (Aβ) peptide can cause neurotoxicity in Alzheimer's disease (AD). The main purpose of the present study is to investigate the protective role of asiatic acid (AA) against Aβ25-35-induced neurotoxicity in neuronally differentiated PC12 cells. Differentiated PC12 cells were pretreated with 5, 10 or 20 μM AA before treatment with 20 μM Aβ25-35. The viability and apoptosis of differentiated PC12 cells were determined by MTT assay and Annexin V-FITC/PI double staining, respectively. The mitochondrial membrane potential (MMP) of differentiated PC12 cells was analyzed by JC-1 staining. The expression levels of proteins were detected by western blot analysis. We found that AA significantly increased the viability of differentiated PC12 cells but attenuated the mitochondria-mediated apoptosis dose-dependently when challenging with Aβ25-35. Besides, the results of western blot analysis showed that AA prevented IκBα degradation and p65 nuclear translocation, and promoted the phosphorylation of Akt and GSK-3β in Aβ25-35-treated differentiated PC12 cells. Moreover, LY294002, a specific PI3K inhibitor, was found to abolish the beneficial effects of AA on Aβ25-35-induced apoptosis and tau protein hyperphosphorylation. Our findings demonstrated that AA protects differentiated PC12 cells from Aβ25-35-induced apoptosis and tau protein hyperphosphorylation, which might be partially mediated by the activation of the PI3K/Akt/GSK-3β signaling pathway. SN - 1879-0631 UR - https://www.unboundmedicine.com/medline/citation/30017668/Asiatic_acid_protects_differentiated_PC12_cells_from_Aβ25_35_induced_apoptosis_and_tau_hyperphosphorylation_via_regulating_PI3K/Akt/GSK_3β_signaling_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(18)30394-1 DB - PRIME DP - Unbound Medicine ER -