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Sensitive Detection of Exosomal Proteins via a Compact Surface Plasmon Resonance Biosensor for Cancer Diagnosis.
ACS Sens. 2018 08 24; 3(8):1471-1479.AS

Abstract

Exosomes are small extracellular vesicles released by cells for cell-cell communication. They play important roles in cancer development, metastasis, and drug resistance. Exosomal proteins have been demonstrated by many studies as promising biomarkers for cancer screening, diagnosis, and monitoring. Among many detection techniques, surface plasmon resonance (SPR) is a highly sensitive, label-free, and real-time optical detection method. Commercial prism-based wavelength/angular-modulated SPR sensors afford high sensitivity and resolution, but their large footprint and high cost limit their adaptability for clinical settings. Recently, a nanoplasmonic exosome (nPLEX) assay was developed to detect exosomal proteins for ovarian cancer diagnosis. However, comparing with conventional SPR biosensors, the broad applications of nanoplasmonic biosensors are limited by the difficult and expensive fabrication of nanostructures. We have developed an intensity-modulated, compact SPR biosensor (25 cm × 10 cm × 25 cm) which uses a conventional SPR sensing mechanism and does not require nanostructure fabrication. Calibration from glycerol showed that the compact SPR biosensor offered sensitivity of 9.258 × 103%/RIU and resolution of 8.311 × 10-6 RIU. We have demonstrated the feasibility of the compact SPR biosensor in lung cancer diagnosis using exosomal epidermal growth factor receptor (EGFR) and programmed death-ligand 1 (PD-L1) as biomarkers. It detected a higher level of exosomal EGFR from A549 nonsmall cell lung cancer (NSCLC) cells than BEAS-2B normal cells. With human serum samples, the compact SPR biosensor detected similar levels of exosomal EGFR in NSCLC patients and normal controls, and higher expression of exosomal PD-L1 in NSCLC patients than normal controls. The compact SPR biosensor showed higher detection sensitivity than ELISA and similar sensing accuracy as ELISA. It is a simple and user-friendly sensing platform, which may serve as an in vitro diagnostic test for cancer.

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Authors+Show Affiliations

Liu C
No affiliation info available
Zeng X
No affiliation info available
An Z
No affiliation info available
Yang Y
No affiliation info available
Eisenbaum M
No affiliation info available
Gu X
Department of General Surgery , Huashan Hospital, Fudan University , Shanghai , 200040 China.
Jornet JM
No affiliation info available
Dy GK
Department of Medicine , Roswell Park Comprehensive Cancer Center , Buffalo , New York 14263 , United States.
Reid ME
Department of Medicine , Roswell Park Comprehensive Cancer Center , Buffalo , New York 14263 , United States.
Gan Q
No affiliation info available
Wu Y
No affiliation info available

MeSH

AgedB7-H1 AntigenBiomarkers, TumorBiosensing TechniquesCarcinoma, Non-Small-Cell LungCell Line, TumorErbB ReceptorsExosomesFemaleHumansLung NeoplasmsMaleMiddle AgedProtein Array AnalysisSurface Plasmon ResonanceSurface Properties

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

30019892

Citation

Liu, Chang, et al. "Sensitive Detection of Exosomal Proteins Via a Compact Surface Plasmon Resonance Biosensor for Cancer Diagnosis." ACS Sensors, vol. 3, no. 8, 2018, pp. 1471-1479.
Liu C, Zeng X, An Z, et al. Sensitive Detection of Exosomal Proteins via a Compact Surface Plasmon Resonance Biosensor for Cancer Diagnosis. ACS Sens. 2018;3(8):1471-1479.
Liu, C., Zeng, X., An, Z., Yang, Y., Eisenbaum, M., Gu, X., Jornet, J. M., Dy, G. K., Reid, M. E., Gan, Q., & Wu, Y. (2018). Sensitive Detection of Exosomal Proteins via a Compact Surface Plasmon Resonance Biosensor for Cancer Diagnosis. ACS Sensors, 3(8), 1471-1479. https://doi.org/10.1021/acssensors.8b00230
Liu C, et al. Sensitive Detection of Exosomal Proteins Via a Compact Surface Plasmon Resonance Biosensor for Cancer Diagnosis. ACS Sens. 2018 08 24;3(8):1471-1479. PubMed PMID: 30019892.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitive Detection of Exosomal Proteins via a Compact Surface Plasmon Resonance Biosensor for Cancer Diagnosis. AU - Liu,Chang, AU - Zeng,Xie, AU - An,Zijian, AU - Yang,Yunchen, AU - Eisenbaum,Maxwell, AU - Gu,Xiaodong, AU - Jornet,Josep M, AU - Dy,Grace K, AU - Reid,Mary E, AU - Gan,Qiaoqiang, AU - Wu,Yun, Y1 - 2018/07/31/ PY - 2018/7/19/pubmed PY - 2019/9/19/medline PY - 2018/7/19/entrez KW - biosensor KW - cancer KW - exosome KW - in vitro diagnostics KW - surface plasmon resonance SP - 1471 EP - 1479 JF - ACS sensors JO - ACS Sens VL - 3 IS - 8 N2 - Exosomes are small extracellular vesicles released by cells for cell-cell communication. They play important roles in cancer development, metastasis, and drug resistance. Exosomal proteins have been demonstrated by many studies as promising biomarkers for cancer screening, diagnosis, and monitoring. Among many detection techniques, surface plasmon resonance (SPR) is a highly sensitive, label-free, and real-time optical detection method. Commercial prism-based wavelength/angular-modulated SPR sensors afford high sensitivity and resolution, but their large footprint and high cost limit their adaptability for clinical settings. Recently, a nanoplasmonic exosome (nPLEX) assay was developed to detect exosomal proteins for ovarian cancer diagnosis. However, comparing with conventional SPR biosensors, the broad applications of nanoplasmonic biosensors are limited by the difficult and expensive fabrication of nanostructures. We have developed an intensity-modulated, compact SPR biosensor (25 cm × 10 cm × 25 cm) which uses a conventional SPR sensing mechanism and does not require nanostructure fabrication. Calibration from glycerol showed that the compact SPR biosensor offered sensitivity of 9.258 × 103%/RIU and resolution of 8.311 × 10-6 RIU. We have demonstrated the feasibility of the compact SPR biosensor in lung cancer diagnosis using exosomal epidermal growth factor receptor (EGFR) and programmed death-ligand 1 (PD-L1) as biomarkers. It detected a higher level of exosomal EGFR from A549 nonsmall cell lung cancer (NSCLC) cells than BEAS-2B normal cells. With human serum samples, the compact SPR biosensor detected similar levels of exosomal EGFR in NSCLC patients and normal controls, and higher expression of exosomal PD-L1 in NSCLC patients than normal controls. The compact SPR biosensor showed higher detection sensitivity than ELISA and similar sensing accuracy as ELISA. It is a simple and user-friendly sensing platform, which may serve as an in vitro diagnostic test for cancer. SN - 2379-3694 UR - https://www.unboundmedicine.com/medline/citation/30019892/Sensitive_Detection_of_Exosomal_Proteins_via_a_Compact_Surface_Plasmon_Resonance_Biosensor_for_Cancer_Diagnosis_ DB - PRIME DP - Unbound Medicine ER -