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Bacterial vectors which confer resistance to kanamycin.
Biochem Biophys Res Commun 1985; 133(3):1112-8BB

Abstract

On the base of plasmid pLD720 (a deletion derivative of the cosmid vector pHC79) a number of hybrid plasmids which confer in Escherichia coli cells the kanamycin resistance was constructed. All hybrid plasmids contain the promoterless part of kanamycin resistance gene (which codes for aminoglycoside 3'-phosphotransferase II) from transposon Tn5. The Km gene expression is driven by a promoters situated on pLD720. The hybrid plasmids pLD723, pLD724 and pLD728 contain a complete DNA sequences of plasmids pC194 or pE194 from Staphylococcus aureus that permits them to replicate into Bacillus subtilis as well. However, no expression of the Km gene in Bacillus subtilis was observed. There is a unical Bgl II site on pLD728 is front of the beginning of a Km gene structural part. This property of pLD728 may be useful when cloning in this plasmid a promoter sequences of different species.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

3002365

Citation

Polumienko, A L., et al. "Bacterial Vectors Which Confer Resistance to Kanamycin." Biochemical and Biophysical Research Communications, vol. 133, no. 3, 1985, pp. 1112-8.
Polumienko AL, Grigor'eva SP, Lukashova LI, et al. Bacterial vectors which confer resistance to kanamycin. Biochem Biophys Res Commun. 1985;133(3):1112-8.
Polumienko, A. L., Grigor'eva, S. P., Lukashova, L. I., Lobach, J. B., Riybaltovskaja, N. B., Lushnikov, A. A., & Domaradskij, I. V. (1985). Bacterial vectors which confer resistance to kanamycin. Biochemical and Biophysical Research Communications, 133(3), pp. 1112-8.
Polumienko AL, et al. Bacterial Vectors Which Confer Resistance to Kanamycin. Biochem Biophys Res Commun. 1985 Dec 31;133(3):1112-8. PubMed PMID: 3002365.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bacterial vectors which confer resistance to kanamycin. AU - Polumienko,A L, AU - Grigor'eva,S P, AU - Lukashova,L I, AU - Lobach,J B, AU - Riybaltovskaja,N B, AU - Lushnikov,A A, AU - Domaradskij,I V, PY - 1985/12/31/pubmed PY - 1985/12/31/medline PY - 1985/12/31/entrez SP - 1112 EP - 8 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 133 IS - 3 N2 - On the base of plasmid pLD720 (a deletion derivative of the cosmid vector pHC79) a number of hybrid plasmids which confer in Escherichia coli cells the kanamycin resistance was constructed. All hybrid plasmids contain the promoterless part of kanamycin resistance gene (which codes for aminoglycoside 3'-phosphotransferase II) from transposon Tn5. The Km gene expression is driven by a promoters situated on pLD720. The hybrid plasmids pLD723, pLD724 and pLD728 contain a complete DNA sequences of plasmids pC194 or pE194 from Staphylococcus aureus that permits them to replicate into Bacillus subtilis as well. However, no expression of the Km gene in Bacillus subtilis was observed. There is a unical Bgl II site on pLD728 is front of the beginning of a Km gene structural part. This property of pLD728 may be useful when cloning in this plasmid a promoter sequences of different species. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/3002365/Bacterial_vectors_which_confer_resistance_to_kanamycin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0006-291X(85)91251-3 DB - PRIME DP - Unbound Medicine ER -