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Commercial cannabis consumer products part 2: HPLC-DAD quantitative analysis of cannabis cannabinoids.
Forensic Sci Int. 2018 Aug; 289:438-447.FS

Abstract

Quantitative analysis for the cannabis cannabinoids such as cannabidiol and Δ9-tetrahydrocannabinol in commercial products is necessary for evaluating label information, and assessing dosages and exposures when the products are consumed. Herein is presented a broadly applicable HPLC-DAD method for the determination of cannabis cannabinoids in commercial consumer products and traditional plant-related substances. The current method provides chromatographic resolution of 11 cannabinoids using a commercial, mixed C18-aromatic functionality stationary phase. The method uses 95% or pure ethanol for extraction, and certain modifications which address specific matrix types are detailed herein. Extensive method validation including precision and accuracy was conducted for five cannabinoids of primary interest (CBD, Δ9-THC, CBDA, THCA, and CBN). UV detection provided excellent sensitivity with limits of quantitation (LOQs) of 10μg/g across cannabinoids. The method was applied to about 60 commercial products representing diverse product types and a broad range of cannabinoids amounts (0.01-350mg/g).

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Publisher Full Text (DOI)

Authors+Show Affiliations

Ciolino LA
Forensic Chemistry Center, Food and Drug Administration, 6751 Steger Drive, Cincinnati, OH, USA. Electronic address: laura.ciolino@fda.hhs.gov.
Ranieri TL
Forensic Chemistry Center, Food and Drug Administration, 6751 Steger Drive, Cincinnati, OH, USA. Electronic address: tracy.ranieri@fda.hhs.gov.
Taylor AM
Forensic Chemistry Center, Food and Drug Administration, 6751 Steger Drive, Cincinnati, OH, USA. Electronic address: allison.taylor@fda.hhs.gov.

MeSH

BeveragesCandyCannabinoidsCannabisChromatography, High Pressure LiquidDronabinolFoodHumansPlant OilsPlant PreparationsSkin Cream

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30025568

Citation

Ciolino, Laura A., et al. "Commercial Cannabis Consumer Products Part 2: HPLC-DAD Quantitative Analysis of Cannabis Cannabinoids." Forensic Science International, vol. 289, 2018, pp. 438-447.
Ciolino LA, Ranieri TL, Taylor AM. Commercial cannabis consumer products part 2: HPLC-DAD quantitative analysis of cannabis cannabinoids. Forensic Sci Int. 2018;289:438-447.
Ciolino, L. A., Ranieri, T. L., & Taylor, A. M. (2018). Commercial cannabis consumer products part 2: HPLC-DAD quantitative analysis of cannabis cannabinoids. Forensic Science International, 289, 438-447. https://doi.org/10.1016/j.forsciint.2018.05.033
Ciolino LA, Ranieri TL, Taylor AM. Commercial Cannabis Consumer Products Part 2: HPLC-DAD Quantitative Analysis of Cannabis Cannabinoids. Forensic Sci Int. 2018;289:438-447. PubMed PMID: 30025568.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Commercial cannabis consumer products part 2: HPLC-DAD quantitative analysis of cannabis cannabinoids. AU - Ciolino,Laura A, AU - Ranieri,Tracy L, AU - Taylor,Allison M, Y1 - 2018/06/30/ PY - 2017/11/16/received PY - 2018/03/06/revised PY - 2018/05/20/accepted PY - 2018/7/21/entrez PY - 2018/7/22/pubmed PY - 2018/8/8/medline KW - Cannabidiol KW - Cannabis cannabinoids KW - Commercial cannabis products KW - High performance liquid chromatography-diode array detection (HPLC-DAD) KW - Δ9-tetrahydrocannabinol SP - 438 EP - 447 JF - Forensic science international JO - Forensic Sci Int VL - 289 N2 - Quantitative analysis for the cannabis cannabinoids such as cannabidiol and Δ9-tetrahydrocannabinol in commercial products is necessary for evaluating label information, and assessing dosages and exposures when the products are consumed. Herein is presented a broadly applicable HPLC-DAD method for the determination of cannabis cannabinoids in commercial consumer products and traditional plant-related substances. The current method provides chromatographic resolution of 11 cannabinoids using a commercial, mixed C18-aromatic functionality stationary phase. The method uses 95% or pure ethanol for extraction, and certain modifications which address specific matrix types are detailed herein. Extensive method validation including precision and accuracy was conducted for five cannabinoids of primary interest (CBD, Δ9-THC, CBDA, THCA, and CBN). UV detection provided excellent sensitivity with limits of quantitation (LOQs) of 10μg/g across cannabinoids. The method was applied to about 60 commercial products representing diverse product types and a broad range of cannabinoids amounts (0.01-350mg/g). SN - 1872-6283 UR - https://www.unboundmedicine.com/medline/citation/30025568/Commercial_cannabis_consumer_products_part_2:_HPLC_DAD_quantitative_analysis_of_cannabis_cannabinoids_ DB - PRIME DP - Unbound Medicine ER -