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Comparative in vivo and in vitro study of the influence of experimental diabetes on rat liver linoleic acid delta 6- and delta 5-desaturation.
Enzyme. 1985; 34(1):1-14.E

Abstract

Rats with experimental diabetes were administered in vivo a tracer dose of either [1-14C]-linoleic, [1-14C]-gamma-linolenic or [2-14C]-dihomo-gamma-linolenic acid and sacrificed 48 h later. With all three radioactive precursors, the radioactivity incorporated into arachidonic acid was lower in experimental diabetes, compared to nondiabetic rats similarly treated, while the weights of hepatic arachidonic acid were not significantly affected by the diabetic state. Streptozotocin-treated rats were administered moderate or excessive quantities of protamine-zinc-insulin. Streptozotocin diabetes inhibits rat liver homogenate [2-14C]-dihomo-gamma-linolenic acid delta 5-desaturation; only moderate injections of protamine-zinc-insulin restore the in vitro delta 5-desaturation. These results suggest that experimental diabetes, a reported inhibitor of delta 6-desaturation, also causes partial inhibition of delta 5-desaturation in rat liver; this suggests that dihomo-gamma-linolenic acid desaturation, a secondary regulatory step in linoleic acid metabolism, may be restored through an optimum insulin therapy.

Authors

No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3002783

Citation

Poisson, J P.. "Comparative in Vivo and in Vitro Study of the Influence of Experimental Diabetes On Rat Liver Linoleic Acid Delta 6- and Delta 5-desaturation." Enzyme, vol. 34, no. 1, 1985, pp. 1-14.
Poisson JP. Comparative in vivo and in vitro study of the influence of experimental diabetes on rat liver linoleic acid delta 6- and delta 5-desaturation. Enzyme. 1985;34(1):1-14.
Poisson, J. P. (1985). Comparative in vivo and in vitro study of the influence of experimental diabetes on rat liver linoleic acid delta 6- and delta 5-desaturation. Enzyme, 34(1), 1-14.
Poisson JP. Comparative in Vivo and in Vitro Study of the Influence of Experimental Diabetes On Rat Liver Linoleic Acid Delta 6- and Delta 5-desaturation. Enzyme. 1985;34(1):1-14. PubMed PMID: 3002783.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative in vivo and in vitro study of the influence of experimental diabetes on rat liver linoleic acid delta 6- and delta 5-desaturation. A1 - Poisson,J P, PY - 1985/1/1/pubmed PY - 1985/1/1/medline PY - 1985/1/1/entrez SP - 1 EP - 14 JF - Enzyme JO - Enzyme VL - 34 IS - 1 N2 - Rats with experimental diabetes were administered in vivo a tracer dose of either [1-14C]-linoleic, [1-14C]-gamma-linolenic or [2-14C]-dihomo-gamma-linolenic acid and sacrificed 48 h later. With all three radioactive precursors, the radioactivity incorporated into arachidonic acid was lower in experimental diabetes, compared to nondiabetic rats similarly treated, while the weights of hepatic arachidonic acid were not significantly affected by the diabetic state. Streptozotocin-treated rats were administered moderate or excessive quantities of protamine-zinc-insulin. Streptozotocin diabetes inhibits rat liver homogenate [2-14C]-dihomo-gamma-linolenic acid delta 5-desaturation; only moderate injections of protamine-zinc-insulin restore the in vitro delta 5-desaturation. These results suggest that experimental diabetes, a reported inhibitor of delta 6-desaturation, also causes partial inhibition of delta 5-desaturation in rat liver; this suggests that dihomo-gamma-linolenic acid desaturation, a secondary regulatory step in linoleic acid metabolism, may be restored through an optimum insulin therapy. SN - 0013-9432 UR - https://www.unboundmedicine.com/medline/citation/3002783/Comparative_in_vivo_and_in_vitro_study_of_the_influence_of_experimental_diabetes_on_rat_liver_linoleic_acid_delta_6__and_delta_5_desaturation_ DB - PRIME DP - Unbound Medicine ER -